A Fluorescence Detection Method of Amphiphilic Monolayer Molecular Film
A fluorescence detection and molecular film technology, applied in the field of fluorescence detection, can solve the problems of inaccurate observation structure, damaged sample surface, non-reusable, etc., and achieve the effect of clear results, low cost and saving detection cost
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Embodiment 1
[0027] S1: Preparation of amphiphilic monolayer molecular membranes by conventional membrane production methods;
[0028] In this example, LB technology was used to prepare a mixed monolayer membrane of sphingomyelin / 1 palmitic acid-2 oleic acid-phosphatidylcholine / cholesterol (hereinafter referred to as BSM / POPC / Chol).
[0029] Dissolve BSM / POPC / Chol (2:2:1, mol:mol:mol) in chloroform and methanol (10:1, V:V) to form a mixed lipid solution with a concentration of 0.01mmol / L. In KSV-Minitrough Prepare in the instrument (Finland KSV company, tank: 323mm×75mm). Use a micro-injector to drop 50uL of the mixed lipid solution onto the surface of pure water and let it spread automatically. After 30 minutes, wait for the organic solvent on the liquid surface to completely volatilize , The film was pressed at a speed of 10mm / min to obtain a BSM / POPC / Chol mixed monolayer molecular film with a thickness of 3nm.
[0030] S2: Under a fixed film pressure (1mN / m~55mN / m), the monolayer is transferr...
Embodiment 2
[0035] S1: Preparation of amphiphilic monolayer molecular membranes by conventional membrane production methods;
[0036] The sphingomyelin and 1 palmitic acid-2 oleic acid-phosphatidylcholine (hereinafter referred to as BSM / POPC) were mixed in a molar ratio of 1:1 and dissolved in chloroform and methanol (3:1, V:V). ) Was formed into a mixed lipid solution with a concentration of 0.5mmol / L, prepared in the KSV-Minitrough instrument (KSV, Finland, tank: 323mm×75mm), and 20uL of the mixed lipid solution was added dropwise to pure water with a micro-injector The surface is allowed to spread automatically. After 15 minutes, after the organic solvent on the liquid surface is completely volatilized, the film is pressed at a speed of 10 mm / min to obtain a BSM / POPC hybrid monolayer molecular film with a thickness of 3 nm.
[0037] S2: Under a fixed film pressure (1mN / m~55mN / m), the monolayer is transferred to the newly dissociated ultra-thin mica substrate using the vertical film method a...
Embodiment 3
[0042] S1: Preparation of amphiphilic monolayer molecular membranes by conventional membrane production methods;
[0043] Dissolve sterol molecules in chloroform and methanol (10:1, V:V) to form a lipid solution with a concentration of 0.01 mmol / L, and prepare it in a KSV-Minitrough instrument (KSV, Finland, tank: 323mm×75mm) , Use a micro-injector to drop 50uL of the lipid solution onto the surface of the pure water and let it spread automatically. After 30 minutes, after the organic solvent on the surface of the liquid has completely evaporated, press the film at a speed of 20mm / min to obtain a sterol monolayer Molecular membrane.
[0044] S2: Under a fixed film pressure (1mN / m~55mN / m), the monolayer is transferred to the newly dissociated ultra-thin mica substrate with a thickness of 0.01mm using the vertical film method at a speed of 15-25mm / min , The film forming temperature is controlled at 21±0.5℃;
[0045] S3: Drop a drop of water on the cleaned cover glass with a thickness...
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