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Optimal cystatin C detection kit and preparation method thereof

A technology for detecting kits and cystatin, which is applied in the field of biomedicine, can solve problems such as the inability to guarantee product stability and accuracy, the limitation of professional skills of operators, and complicated operations, so as to achieve stable test results, improve accuracy and resist Interference ability, simple operation effect

Inactive Publication Date: 2017-07-07
吉林省富生医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of the existing cystatin C detection kit also has the shortcomings of complicated operation, long time consumption, limitation of professional skills of the operator, and inability to guarantee the stability and accuracy of the product

Method used

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  • Optimal cystatin C detection kit and preparation method thereof
  • Optimal cystatin C detection kit and preparation method thereof
  • Optimal cystatin C detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The kit of the present invention is exemplified as a double reagent, wherein:

[0063] Reagent R1

[0064]

[0065] Reagent R2:

[0066]

Embodiment 2

[0068] As a preference, the present invention discloses a preferred cystatin C assay kit, which comprises independent reagents R1 and R2 to form the kit, and the components and corresponding contents included are:

[0069]

[0070]

[0071] Polystyrene microspheres coated with cystatin C antibody and β-cyclodextrin particles, particle size 100nm, concentration 1%.

[0072] Calibrator:

[0073]

[0074] According to the required concentration of the cystatin C reference calibrator, the corresponding cystatin C standard was added to the above buffer respectively to prepare a set of calibrator with different concentrations.

Embodiment 3

[0076] How to use the kit:

[0077] 1. Reagent preparation, the reagent is a liquid double reagent, ready to use after opening the bottle, of which:

[0078] (1), preparation of reagent R1:

[0079] Configuration buffer 1: phosphate buffer 10mmol / L;

[0080] Add bovine serum albumin 10g / L, Tween 20 0.2mmol / L, polyethylene glycol 3.5mmol / L, sodium chloride 0.12mol / L, sodium azide 10mmol / L into buffer 1, stir and mix evenly, The reagent R1 is obtained;

[0081] (2), the preparation of reagent R2:

[0082] Step 1: Dialyze the Cystatin C antibody at 4°C, and then dilute the Cystatin C antibody to 2 mg / ml with phosphate buffer to obtain the Cystatin C antibody dilution; Dilute the polystyrene microspheres with purified water Centrifuge and wash 3 times;

[0083] Step 2: Dilute the polystyrene microspheres washed in Step 1 with phosphate buffer solution to a mass concentration of 1.0%, then add 0.3% β-cyclodextrin, stir and react at room temperature for 30 minutes, and the reac...

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Abstract

The invention relates to a cystatin C detection kit and a preparation method thereof. The kit includes reagents R1 and R2 which are independent of each other. The reagent R1 mainly consists of a buffer solution 1, an inorganic salt ion 1, a stabilizer 1, Preservative 1, solubilizer 1 and accelerator 1; the reagent R2 is mainly composed of polystyrene microspheres of cross-linked cystatin C antibody, buffer 2, stabilizer 2, solubilizer 2, preservative 2 and protection Reagent 2, the polystyrene microspheres are connected to the cystatin C antibody through physical adsorption or chemical cross-linking, and are encapsulated by β-cyclodextrin. The cystatin C detection kit of the invention has the advantages of low preparation cost, good stability, easy storage, good data accuracy and high detection sensitivity, and can be widely used in clinical biochemical instruments.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preferred cystatin C detection kit and a preparation method thereof. Background technique [0002] Kidney disease is a common clinical disease. So far, early diagnosis, early treatment and reversal of damaged kidney function are the only ways to prevent kidney disease from worsening. The clinical evaluation of the progress and severity of renal disease generally takes renal function as a reference, and glomerular filtration rate (GFR) is the most important indicator reflecting renal function. [0003] Cystatin C is a cysteine ​​protease inhibitor, also known as γ-trace protein or γ-postglobulin, and is a low molecular weight, basic non-glycosylated protein with a molecular weight of 13.3KD. Composed of 122 amino acid residues, it can be produced by all nucleated cells in the body with a constant production rate. Cystatin C in the circulation is only cleared by...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/31
CPCG01N33/6893G01N21/31G01N2800/347
Inventor 张海悦孙景春闫小娟魏滢王姝韩永双韩峰刘玉娟
Owner 吉林省富生医疗器械有限公司
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