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Preparation method and application of spirulina whole active substances

A technology for active substances and spirulina, which is applied in the field of preparation of all active substances of spirulina, can solve the problems of low utilization rate and incomplete preparation of active substances, and achieves the effects of high repeatability, strong practicability, and stable and reliable process.

Inactive Publication Date: 2017-07-07
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are problems such as incomplete preparation of active substances of spirulina and low utilization rate in the technology of this field
At present, there is no report on the method of extracting spirulina with sequential solvents (from small to large polarity) to prepare active substances, and this method has good development prospects for large-scale industrial production

Method used

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  • Preparation method and application of spirulina whole active substances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for preparing the active substance of spirulina, using spirulina as raw material, using solvent extraction with different polarities (from small to large) and enzymatic hydrolysis with compound protease to prepare the active substance of spirulina:

[0027] First extract with 85wt% ethanol, then extract the residue of 85wt% ethanol extraction with 65wt% ethanol, then extract the residue of 65wt% ethanol with water, and finally enzymolyze the water extraction residue, and combine the above alcohol extraction, water extraction and enzymolysis The obtained extract was centrifuged, filtered, decompressed, concentrated and dried to obtain 85wt% ethanol extract of spirulina, 65wt% ethanol extract of spirulina, water extract of spirulina and enzymatic hydrolyzate of spirulina, namely: spirulina Full active substance; Concretely comprises the following steps:

[0028] A. Spirulina fine powder was first extracted with 85wt% ethanol at 45°C for 3 hours by ultrasonic-micr...

Embodiment 2

[0035] A method for preparing the active substance of spirulina, using spirulina as raw material, using solvent extraction with different polarities (from small to large) and enzymatic hydrolysis with compound protease to prepare the active substance of spirulina:

[0036] First extract with 95wt% ethanol, then extract the residue of 95wt% ethanol extraction with 55wt% ethanol, then extract the residue of 55wt% ethanol with water, and finally enzymolyze the residue of water extraction, the above alcohol extraction, water extraction and enzymolysis The obtained extracts were centrifuged, filtered, decompressed, concentrated and dried to obtain Spirulina 95wt% ethanol extract, Spirulina 55wt% ethanol extract, Spirulina water extract and Spirulina enzymatic hydrolyzate, namely: Spirulina Full active substance; Concretely comprises the following steps:

[0037] A. Spirulina fine powder was extracted with 95wt% ethanol at 70°C for 0.5h by ultrasonic-microwave co-extraction, and fil...

Embodiment 3

[0044] A method for preparing the whole active substance of spirulina, using spirulina as raw material, using solvents of different polarities (from small to large) to extract and prepare the whole active substance of spirulina:

[0045] First extract with 90wt% ethanol, then extract the residue of 90wt% ethanol extraction with 60wt% ethanol, then extract the residue of 60wt% ethanol with water, and finally enzymolyze the water extraction residue, and combine the above alcohol extraction, water extraction and enzymolysis The extract obtained is centrifuged, filtered, decompressed, concentrated and dried to obtain 90wt% ethanol extract of spirulina, 60wt% ethanol extract of spirulina, water extract of spirulina and enzymatic hydrolyzate of spirulina, namely: spirulina Full active substance; Concretely comprises the following steps:

[0046] A. Use 90wt% ethanol to extract spirulina fine powder at 55°C for 1.5h with ultrasonic assistance, and filter to obtain 90wt% ethanol extra...

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Abstract

The invention discloses a preparation method and application of spirulina whole active substances. Spirulina is used as a raw material. The spirulina whole active substances are extracted and prepared by the method of performing extraction with solvents of different polarities (from small to large), performing enzymolysis with compound proteinases and the like, and are prepared through the following steps of firstly performing extraction with 85-95wt% ethanol, then performing extraction with 55-65wt% ethanol, then performing water extraction, finally performing enzymolysis on water extraction residues, then performing centrifuging, performing filtration, performing decompression, performing concentration, and performing drying so as to obtain a spirulina 85-95wt% ethanol extract, a spirulina 55-65wt% ethanol extract, a spirulina water extract and a spirulina zymolyte, namely the spirulina whole active substances. Pharmacodynamics experiments verify that the whole active substances can significantly improve lipid metabolism disorder induced by high fat diet, and specifically, can significantly reduce the level of total cholesterol (TC), the level of triglyceride (TG) and the level of low density lipoprotein cholesterol (LDL-C) in serum.

Description

technical field [0001] The invention belongs to the field of extracting active substances from spirulina, and in particular relates to a preparation method and application of active substances from spirulina. Background technique [0002] Spirulina is the best pure natural protein food source discovered by human beings, and its protein content is as high as 60%-70%. , vitamins, minerals, chloroplasts, carotene and polysaccharides, so it is called "21st century food". Studies have found that spirulina has significant immune regulation, anti-fatigue, blood-nourishing, hypoxia resistance, lowering blood pressure, lowering blood sugar and blood lipids, inhibiting tumors, protecting liver and liver, promoting the absorption of trace minerals, losing weight and overcoming malnutrition, etc. Various health functions. [0003] Diabetes and hypertension are common and frequently-occurring diseases in my country, and are the third most harmful diseases to human health in the world a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A23L29/00
CPCA23V2002/00A23V2250/21A23V2200/30
Inventor 刘斌李田田潘雨阳贾瑞博郭伟灵赵超陈珍金江小琴黄梓芮
Owner FUJIAN AGRI & FORESTRY UNIV
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