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Antrodia camphorate growth promoter and method for fast producing antrodia camphorate through fermentation

A technology of growth promoter and fermentation method, which is applied in the field of rapid production of Antrodia camphorata mycelium and fruiting bodies, can solve the problems of high production cost, easy pollution of miscellaneous bacteria, and long cultivation period, so as to increase production cost and reduce pollution of miscellaneous substances. effect on the probability of bacteria

Inactive Publication Date: 2017-06-23
JIANGNAN UNIV
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AI Technical Summary

Problems solved by technology

The solid-state cultivation of Antrodia camphorata is similar to the growth environment of wild Antrodia camphorata, and the morphology and active ingredients of the cultured products are similar to those of wild Antrodia camphorata. The main solid-state cultivation methods are solid-state fermentation in space bags and triangular flasks, but the cultivation period is long and it is easy to pollute impurities. Bacteria, high production cost
[0004] Patent CN101803534B provides a large-scale solid culture method of Antrodia camphorata, which can be used for commercial planting on a large scale through inoculation and cultivation of space packs. The fruiting body of Antrodia camphorata obtained is in the shape of a cone and weighs about 0.2-1.0kg, but the cultivation period is longer , from May to August

Method used

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  • Antrodia camphorate growth promoter and method for fast producing antrodia camphorate through fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Antrodia camphorata mycelium is transferred to PDA slant medium, cultured at 24-30°C for 10-20 days;

[0021] 2. Antrodia camphorata slant strains are inserted into the seed medium, and cultivated at 24-30°C for 10-20 days to obtain asexual spore-containing seed liquid;

[0022] Seed medium (g / L): glucose 15-25, yeast powder 0.5-2, magnesium sulfate 0.5-2, potassium dihydrogen phosphate 1-4, pH 4.5;

[0023] 3. The composition of Antrodia camphorata solid-state fermentation medium in terms of weight percentage is 30% to 60% of camphor wood chips, 5% to 20% of bran, 5% to 20% of bean cake powder, 0.15% to 3% of magnesium sulfate, and diphosphate Potassium hydrogen 0.15% to 3%, spray an aqueous solution containing 30 μM fermentation accelerator vanillin, adjust the water content to 35-65%, mix all materials to prepare space bags, each bag is 1.0 to 5.0 kg. Sterilize the space pack at 100-180°C for 2-5 hours, and cool to room temperature for later use.

[0024] 4. Ino...

Embodiment 2

[0027] 1. Antrodia camphorata mycelium is transferred to PDA slant medium, cultured at 24-30°C for 10-20 days;

[0028] 2. Antrodia camphorata slant strains are inserted into the seed medium, and cultivated at 24-30°C for 10-20 days to obtain asexual spore-containing seed liquid;

[0029] Seed medium (g / L): glucose 15-25, yeast powder 0.5-2, magnesium sulfate 0.5-2, potassium dihydrogen phosphate 1-4, pH 4.5;

[0030] 3. Antrodia camphorata liquid fermentation culture, the Antrodia camphorata seed liquid is inoculated in the Antrodia camphorata liquid fermentation medium (glucose 20g / L, yeast powder 2g / L, magnesium sulfate 1.5g / L, potassium dihydrogen phosphate 3.0% by volume ratio) g / L, pH 4.5), add 30 μM ferulic acid to the liquid fermentation medium of Antrodia camphorata, culture at 26°C and 150 rpm for 10 days, collect mycelia of Antrodia camphorata, dry and pulverize.

[0031] 4. Weigh 0.05g of Antrodia camphorata mycelium and sporocarp powder after drying and crushing,...

Embodiment 3

[0033] Adopt the method for cultivating the fruiting body of Antrodia camphorata without adding a fermentation accelerator, and the specific steps are as follows:

[0034] 1. Antrodia camphorata mycelium is transferred to PDA slant medium, cultured at 24-30°C for 10-20 days;

[0035] 2. Antrodia camphorata slant strains are inserted into the seed medium, and cultivated at 24-30°C for 10-20 days to obtain asexual spore-containing seed liquid;

[0036] Seed medium (g / L): glucose 15-25, yeast powder 0.5-2, magnesium sulfate 0.5-2, potassium dihydrogen phosphate 1-4, pH 4.5;

[0037] 3. The composition of Antrodia camphorata solid-state fermentation medium in terms of weight percentage is 30% to 60% of camphor wood chips, 5% to 20% of bran, 5% to 20% of bean cake powder, 0.15% to 3% of magnesium sulfate, diphosphate Potassium hydrogen 0.15% to 3%, adjust the water content to 35-65%, mix all materials to prepare space bags, each bag is 1.0 to 5.0kg. Sterilize the space pack at 10...

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Abstract

The invention relates to an antrodia camphorate growth promoter and a method for fast producing antrodia camphorate through fermentation. The method comprises the processes of inclined surface culture, liquid state seed preparation, liquid state fermentation and solid state fermentation. In each fermentation process, one kind or the combination of several kinds of aromatic compounds such as vanillina can be added, so that the culture period of producing antrodia camphorate mycelia and encarpium through fermentation can be greatly shortened; the content of bioactive substances of antrodia camphorate triterpene is improved. Compared with the existing study and report technology, the antrodia camphorate growth promoter and the method have the advantages that the process is simple; the efficiency is high; the trace growth promoter is used; the goal of fast producing the antrodia camphorate mycelia and encarpium can be achieved; the fermentation period is reduced by more than 50 percent through being compared with that of a conventional method; the content of the antrodia camphorate triterpene is improved by 60 percent or higher; the production cost is reduced; good industrial application prospects are realized.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a method of adding a growth promoter or a combination of several growth promoters in the fermentation process, and using methods such as liquid fermentation, medium solid fermentation and space bag solid fermentation to rapidly produce Antrodia camphorata Method for mycelia and fruiting bodies. Background technique [0002] Antrodia camphorata (Antrodia camphorata) is a rare medicinal fungus belonging to the genus Plepidobacteria with high host specificity. It only grows on the inner wall of the decayed trunk of the evergreen broad-leaved tree Cinnamomum camphorata unique to Taiwan. In history, Taiwan people used it as a traditional Chinese medicine for relieving alcohol, protecting the liver and treating diarrhea. Modern studies have shown that Antrodia camphorata contains a variety of active ingredients, such as triterpenes, polysaccharides, steroids and benzene ring der...

Claims

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Application Information

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IPC IPC(8): C05G3/00C12N1/14C12N1/38A01G1/04C12R1/645
CPCA01G18/00C05B7/00C05G3/00C05G5/20C12N1/14C12N1/38C05D5/00C05F5/002C05F11/00C05F11/08
Inventor 许正宏史劲松陆震鸣朱青耿燕许泓瑜龚劲松钱建瑛
Owner JIANGNAN UNIV
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