Recombinant protein coded by GCRV (grass carp reovirus) II type S9 genes and application thereof

Abstract

The invention discloses a recombinant protein coded by GCRV (grass carp reovirus) II type S9 genes and application thereof. The amino acid sequence of the recombinant protein is shown as SEQ ID NO:2. The nucleotide sequence for coding the protein is shown as SEQ ID NO:1. The obtained recombinant protein has better immunogenicity. Compared with CGRV proteins of other structures, the recombinant protein has the advantage that the valence of specific antibodies generated by immune animals induced by the protein is higher. Further experiments show that when VP6 proteins are used for immunizing grass carps, the grass carps can be induced to generate higher specific antibody; a certain immune protection effect is achieved on the GCRV strong virus plant attack. Therefore the development and the application of the GCRV II type VP6 protein have very important significance on developing novel grass carp hemorragic disease vaccine and immunological detection kits, and a novel effective solution path is provided for grass carp hemorragic diseases.

Description

Technical field [0001] The invention belongs to the field of biological genetic engineering, and specifically relates to a recombinant VP6 antigen protein encoded by a grass carp reovirus type II S9 gene and its application. Background technique [0002] Grass carp hemorrhagic disease caused by grass carp reovirus (GCRV) has caused huge economic losses to the grass carp breeding industry in our country and severely restricted the healthy development of grass carp breeding in our country. GCRV has a double-layered capsid, the average diameter of virus particles is 60nm~70nm, icosahedral symmetry, and no envelope. The genome consists of 11 segments of double-stranded RNA. According to the size of its gene segments, its genome The molecular weight is divided into 3 groups, named L1-L3, M4-M6 and S7-S11. These 11 RNA segments can encode 12 polypeptide proteins, but which genes encode structural proteins, which encode non-structural proteins, and the functions of each encoded protein...

AI Technical Summary

Problems solved by technology

Due to the relatively poor immunogenicity of grass carp reovirus, there is still no effective immunological diagnosis method, especially the lack of serological detection methods to evaluate the immune effect of vaccines

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