Chromosome culture medium for quickly harvesting metaphase cells and application

Abstract

The invention discloses a chromosome culture medium for quickly harvesting metaphase cells. The chromosome culture medium for quickly harvesting the metaphase cells comprises the following active ingredients: an RPMI 1640 powder culture medium, calf serum, penicillin sodium, streptomycin sulphate, sodium bicarbonate and phytohemagglutinin(PHA), wherein the contents of various active ingredients are respectively as follows: 10.4 g / L of the RPMI 1640 powder culture medium, 100 ml / L of the calf serum, 400 to 600 mu l / L of the penicillin sodium, 400 to 600 mu l / L of the streptomycin sulphate, 2 g / L of the sodium bicarbonate and 100 to 120 mg / L of the phytohemagglutinin(PHA). The effects of different components and the PHA are fully played; a large amount of cells in a metaphase can be obtained; chromosome nuclear type analysis and clinical diagnosis are conveniently performed; meanwhile, the chromosome culture medium can support quickly and effectively harvesting the metaphase cells and shorten the processing time of colchicines, so that the time required for preparing chromosomes is shortened; help is provided for performing the chromosome nuclear type analysis and genetic diagnosis largely.

Description

technical field [0001] The invention relates to a chromosome culture medium, in particular to a chromosome culture medium for rapidly harvesting metaphase cells and an application thereof, belonging to the technical fields of cell biology and human medical genetics. Background technique [0002] Any abnormal number of chromosomes, even small structural aberrations, such as duplications, deletions, translocations, etc., will lead to the increase or absence of genes, resulting in corresponding clinical phenotypes. Such as multiple dysmorphic syndrome, symptoms include multiple deformities, mental retardation and abnormal growth and development, in addition there may be some changes in skin texture. Chromosomal aberrations can also lead to fetal stillbirth or miscarriage. Carriers of balanced chromosomal translocations with normal appearance may cause miscarriage, stillbirth, neonatal death or congenital malformation in their offspring. It has been found that more than 50% of...

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Problems solved by technology

[0004] At present, there is no uniform standard for chromosome culture media on the market, and the quality is uneven. Some culture media are of unstable quality, which affects the effect of chromosome preparation and analysis and diagnosis.

Chromosome examination is applicable to a wide range of people and requires a lot of examinations, but the preparation of chromosome specimens takes a long time, which not only affects the generation time of diagnostic results, but also affects the progress of other examinations for patients, and it is not convenient for major hospitals to carry out a large number of chromosome examinations. an examination

In the process of chromosome preparation, it is necessary to add colchicine to terminate the metaphase cells, and the processing time of colchicine is generally longer, which is a time-consuming step in the chromosome preparation process

Although there have been some reports of improved medium, but its application in chromosome preparation, the colchicine treatment time is still relatively long, generally 2-4 hours

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