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Composition and reserve of bone filling material as well as preparation methods and applications of composition and reserve

A filling material and composition technology, used in tissue regeneration, medical science, prosthesis, etc., can solve the problems of inability to promote osteogenesis, high hardness, difficult placement, etc., to promote osteogenic differentiation and osteoblast function, Good shape and easy to shape effect

Inactive Publication Date: 2017-05-31
李倩 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there are following deficiencies in clinical use and osteogenic induction ability: (1) the elastic modulus of bone powder particles is about 1GPa, which is far higher than the recognized elastic substrate hardness (about 0.1MPa) that can induce stem cell osteogenic differentiation; 2) Pure inorganic bone materials cannot promote osteogenesis under chemical or biological factor-induced conditions; (3) In the case of those used as granular bone powder, the granular bone powder still has displacement, migration, difficulty in forming, and difficult placement The disadvantages of this method lead to inconvenient clinical operation and difficult to guarantee the effect of bone grafting.
The elastic modulus of this material is lower than that of Bio-Oss bone powder (about 1 MPa), but it is still higher than the ideal range for inducing osteogenic differentiation of stem cells, and it is expensive, which brings economic burden to patients
[0006] In summary, the existing bone filling materials are difficult to solve the following problems at the same time:
[0007] (1) The bone filling materials commonly used in clinical practice (xenogeneic bone or artificial synthetic bone material) are too hard, which is not suitable for the osteogenic differentiation of mesenchymal stem cells and the maintenance of bone cell function;
[0008] (2) The material only provides physical support for the defect site, and lacks the induction effect of growth factors;
[0009] (3) The plasticity of the material is poor, and it cannot meet the defect transplantation with large area or special shape requirements, and it is not suitable for the individual needs of clinical bone defect treatment.
[0010] In addition, some commercial finished products of bone filling materials are expensive and difficult to be widely used.

Method used

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  • Composition and reserve of bone filling material as well as preparation methods and applications of composition and reserve
  • Composition and reserve of bone filling material as well as preparation methods and applications of composition and reserve
  • Composition and reserve of bone filling material as well as preparation methods and applications of composition and reserve

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] (1) The finished Bio-Oss bone meal (purchased from Geistlich Pharma AG) was ground into powder under sterile conditions, and the ground powder particles were observed under an environmental scanning electron microscope, and the particle size was about 10um-200um. The particle size is just suitable for mixing to make a scaffold material with uniform elasticity and hardness, and to form gaps of appropriate size to facilitate cell growth and bone formation.

[0061] (2) Prepare 5% and 10% aqueous gelatin solutions, stir in a water bath at 50°C to promote dissolution, return to room temperature for use; prepare 5% glutaraldehyde aqueous solution.

[0062] (3) According to the dosage in Table 1, follow the steps below to prepare bone meal-gelatin films with different ratios.

[0063] Steps:

[0064] a. Quickly mix the ground bone meal powder with gelatin solution and glutaraldehyde solution on a glass slide, cover the surface of the mixed colloid with another glass slide, a...

Embodiment 2

[0074] (1) Grind the finished Bio-Oss bone powder (purchased from Geistlich Pharma AG) into a powder under sterile conditions, and the particle size of the ground powder particles is about 10um-200um observed under an environmental scanning electron microscope. The particle size is just suitable for mixing to make a scaffold material with uniform elasticity and hardness, so as to facilitate cell growth and bone formation.

[0075] (2) A gelatin aqueous solution with a concentration of 10% was prepared, stirred in a water bath at 50° C. to promote dissolution, and returned to room temperature for use; a 1% genipin aqueous solution stock solution was prepared (genipin was purchased from Shanghai Shifeng Biotechnology Co., Ltd.).

[0076](3) According to the dosage in Table 2, the gelatin / genipin-bone meal scaffold was prepared according to the following steps. The elastic modulus of the prepared gelatin / genipin-bone meal scaffold is shown in Table 2.

[0077] Steps:

[0078] a...

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Abstract

The invention belongs to the technical field of medical biomaterials and particularly relates to composition and a reserve of a bone filling material as well as preparation methods and applications of the composition and the reserve. The composition of the bone filling material comprises bone meal, gelatin and a crosslinking agent, and has the advantages of being inductive, elastic and good in shaping property. The reserve of the bone filling material is a product prepared from a solution of the composition of the bone filling material through freeze drying. A method for preparing the bone filling material comprises the step of mixing the composition or the reserve of the bone filling material with a medically acceptable solvent. The raw materials are widely sourced, the preparation is simple, and a fillable bone filling material can be provided timely during clinical application. The bone filling material can be widely applied to the field of scientific research, or serve as a medical material for use.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to compositions and preparations of bone filling materials as well as their preparation methods and applications. Background technique [0002] Human bone repair technology has gone through a long period of development, mainly in the biocompatibility of materials has made great improvements, from the original wicker to metal, and then to medical polymers, now bone repair materials have entered the bionic material stage. However, no matter what kind of material it is, there has not been much improvement in clinical use methods. Basically, materials that are not easy to shape, such as granular or strip-shaped, are still used. In clinical use, doctors have high technical requirements, especially for Fractures or non-union bone sutures are very inconvenient to operate. Due to trauma, tumor, pathological disease or other forms of bone trauma and bone implantati...

Claims

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Application Information

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IPC IPC(8): A61L27/12A61L27/22A61L27/50A61L27/56
CPCA61L27/12A61L27/222A61L27/50A61L27/56A61L2430/02
Inventor 李倩杨春赵继志祖岩穆月
Owner 李倩
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