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Composition and application thereof

A composition and bone marrow mesenchymal technology, applied in the field of cells, can solve problems such as limiting the recovery of liver function

Inactive Publication Date: 2017-05-24
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Hwang, S. et al. used bone marrow mesenchymal stem cells and bone marrow mesenchymal stem cells induced by HGF in vitro for 1 week or 2 weeks to treat rats with liver failure. Stem cells and early stages of hepatocyte differentiation may be beneficial for hepatocyte regeneration and restoration of liver function, while transplantation of mature hepatocytes may limit recovery of liver function

Method used

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  • Composition and application thereof
  • Composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Separation and Purification of Bone Marrow Mesenchymal Stem Cells

[0033] Separation:

[0034]Rats were sacrificed by short neck after intraperitoneal injection of heparin. Soak in 75% ethanol solution for 10min. Under the aseptic conditions in the ultra-clean bench, the bilateral cavity bones of the rats were taken out and rinsed twice with PBS. The end of the rat femur was cut off, and the bone marrow cavity was repeatedly washed by aspirating the culture medium with a syringe to clean the bone marrow cavity. Pass the cell suspension through a 70 μm cell strainer to remove bone debris and pooled blood. The cell suspension was collected in a centrifuge tube, washed twice with medium, and centrifuged. Resuspend the cells in DMEM-F12 medium containing 10% FBS, adjust the cell density to 2×105 / ml and inoculate them on a 15cm culture dish, and transfer them to a CO2 incubator with 5% CO2, 37°C, and a saturated humidity of 95% nourish. When the cells were c...

Embodiment 2

[0040] Embodiment 2 builds rat chronic liver failure model

[0041] The model of chronic liver failure in rats was induced by intraperitoneal injection of carbon tetrachloride, and carbon tetrachloride was dissolved in vegetable oil at a ratio of 4:6. Select 60 healthy week-old adult male SD rats, body weight (200 ± 20) g, and randomly divide them into 4 groups: normal group (25 rats), model group (25 rats), cell composition treatment group 1 (20 rats) , cell composition treatment group 2 (20 rats), cell composition treatment group 3 (20 rats) and normal saline group (20 rats). The model group, the cell composition treatment group and the normal saline group were treated with 40% CCl 4 The vegetable oil solution 1ml / kg body weight was injected intraperitoneally every three days for 7 consecutive weeks to induce chronic liver failure in rats in the model group. The normal group was injected intraperitoneally with vegetable oil 1ml / kg body weight every three days for 7 consecu...

Embodiment 3

[0046] Example 3 Preparation of bone marrow mesenchymal stem cell composition

[0047] After identification by flow cytometry and trypan blue staining, the purity was more than 90%, and the total number reached 10 7 Bone marrow mesenchymal stem cells, centrifuged to remove the supernatant, washed 1-2 times with normal saline for injection, centrifuged at 400g for 5min, and collected the cells;

[0048] According to 30μg / ml hepatocyte growth factor, 4μg / ml prostaglandin, 20U / ml insulin, 3mg / ml glucagon, 20% (w / w) albumin, 1.5×10 7 Bone marrow mesenchymal stem cells / kg of rat body weight were resuspended in physiological saline for injection to prepare 1 ml of bone marrow mesenchymal stem cell composition.

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Abstract

The invention relates to the field of cells, and in particular to a composition and an application thereof. The composition consists of a growth factor, albumin and mesenchymal stem cells. The composition is effective in preventing and / or treating chronic liver injuries caused by ischemia, drugs (such as paracetamol), viruses, obesity, non-alcoholic steatohepatitis, biliary obstruction and tumors. The composition can also prevent hepatic failure, retard the progress of chronic hepatic failure and remove harmful toxin.

Description

technical field [0001] The present invention relates to the field of cells, in particular to compositions and applications thereof. Background technique [0002] Liver failure is severe liver damage caused by a variety of factors, leading to serious impairment or decompensation of its synthesis, detoxification, excretion, and biotransformation functions, with coagulation dysfunction, jaundice, hepatic encephalopathy, and ascites as the main manifestations A group of clinical syndromes. In recent years, the number of deaths due to liver failure has gradually increased. According to statistics, about 10,000 people die of liver failure in the United States every year, while about 10,000 patients die of this disease in my country every year. Acute liver failure is an acute and severe liver dysfunction, which is characterized by acute onset, critical condition, various symptoms and extensive necrosis of liver cells. At present, the main means of treatment of liver failure are m...

Claims

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Application Information

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IPC IPC(8): A61K38/38A61P1/16A61K31/5575A61K35/28
CPCA61K38/1833A61K31/5575A61K35/28A61K38/26A61K38/28A61K38/38A61K2300/00
Inventor 葛啸虎陈海佳王一飞黄新珠王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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