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Composition and application thereof

A composition, umbilical cord technology, applied in the field of cells, can solve problems such as patient harm

Inactive Publication Date: 2017-05-31
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The research on the treatment of acute liver failure with bone marrow mesenchymal stem cells has also been reported, but the process of obtaining bone marrow mesenchymal stem cells has caused great harm to patients, and the use of adipose-derived mesenchymal stem cells also has the disadvantage of inducing fatty liver

Method used

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  • Composition and application thereof
  • Composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Separation and Purification of Umbilical Cord Mesenchymal Stem Cells

[0030] separate

[0031] Aseptically take 10 cm of healthy human fetal umbilical cord, soak it in 75% ethanol solution for 5 minutes, and wash it twice with PBS. The umbilical cord adventitia and blood vessels were separated and removed, the umbilical cord Wharton's jelly tissue was cut into 1mm3 tissue pieces, moved to 0.1% collagenase IV, digested at 37°C for 18h, and the cell suspension was passed through a 70μm cell strainer. The cell suspension was collected in a centrifuge tube, washed twice with medium, and centrifuged. Resuspend the cells in DMEM-F12 medium containing 10% FBS, and adjust the cell density to 2×10 5 / ml was inoculated into a 15cm petri dish, and transferred to a CO2 incubator with 5% CO2, 37°C, and a saturated humidity of 95%. Change the medium after 6-7 days of cell culture, and change the medium once every 3 days thereafter until the cells grow to a confluence of...

Embodiment 2

[0037] Embodiment 2 builds rat chronic liver failure model

[0038] The model of chronic liver failure in rats was induced by intraperitoneal injection of carbon tetrachloride, and carbon tetrachloride was dissolved in vegetable oil at a ratio of 4:6. Select 60 healthy week-old adult male SD rats, body weight (200 ± 20) g, and randomly divide them into 4 groups: normal group (25 rats), model group (25 rats), cell composition treatment group (20 rats) and Normal saline group (20 rats). The model group, the cell composition treatment group and the normal saline group were treated with 40% CCl 4 The vegetable oil solution 1ml / kg body weight was injected intraperitoneally every three days for 7 consecutive weeks to induce chronic liver failure in rats in the model group. The normal group was injected intraperitoneally with vegetable oil 1ml / kg body weight every three days for 7 consecutive weeks. Seven weeks after the model was established, 5 rats in the normal group and 5 rats...

Embodiment 3

[0043] Example 3 Preparation of umbilical cord mesenchymal stem cell composition

[0044] After identification by flow cytometry and trypan blue staining, the purity was more than 90%, and the total number reached 10 7 The umbilical cord mesenchymal stem cells were centrifuged to remove the supernatant, washed 1-2 times with normal saline for injection, centrifuged at 400g for 5min, and the cells were collected;

[0045] According to 15% (w / w) albumin, 30 μg / ml hepatocyte growth factor, 4 μg / ml prostaglandin E2 (PGE2), 20 U / ml insulin, 3 mg / ml glucagon, 1.5×10 7 Umbilical cord mesenchymal stem cells / kg rat body weight were resuspended in saline for injection to prepare 1 ml of umbilical cord mesenchymal stem cell composition.

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Abstract

The invention relates to the field of cells, and particularly relates to a composition and an application thereof. The composition contains growth factors, albumin and umbilical cord mesenchymal stem cells. The composition can prevent hepatic failure, slow the progression of chronic hepatic failure and remove harmful toxicity. The cell composition is effective for preventing and / or treating chronic hepatic failure induced by ischemia, drugs such as acetyl aminophenol, viruses, obesity, nonalcoholic steatohepatitis, bile duct obstruction and tumors.

Description

technical field [0001] The present invention relates to the field of cells, in particular to compositions and applications thereof. Background technique [0002] Liver failure is severe liver damage caused by a variety of factors, leading to serious impairment or decompensation of its synthesis, detoxification, excretion, and biotransformation functions, with coagulation dysfunction, jaundice, hepatic encephalopathy, and ascites as the main manifestations A group of clinical syndromes. In recent years, the number of deaths due to liver failure has gradually increased. According to statistics, about 10,000 people die of liver failure in the United States every year, while about 10,000 patients die of this disease in my country every year. Acute liver failure is an acute and severe liver dysfunction, which is characterized by acute onset, critical condition, various symptoms and extensive necrosis of liver cells. At present, the main means of treatment of liver failure are m...

Claims

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Application Information

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IPC IPC(8): A61K38/38A61P1/16A61K38/28A61K31/557A61K35/28A61K38/18A61K38/26
CPCA61K35/28A61K31/557A61K38/1833A61K38/26A61K38/28A61K38/38A61K2300/00
Inventor 葛啸虎陈海佳王一飞黄新珠王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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