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Extraction method of sea cucumber collagen

A sea cucumber collagen and extraction method technology, which is applied in the field of sea cucumber collagen extraction, can solve the problems of small collagen acquisition, long preparation time, and unsuitability for large-scale production, so as to increase economic added value and improve extraction rate , increase the effect of elasticity

Inactive Publication Date: 2017-05-17
嵊州市派特普科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The amount of collagen obtained by this method is not large, the preparation time is long, and it is not suitable for large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for extracting sea cucumber collagen, the specific steps are:

[0020] 1) Deodorization: Cut sea cucumbers into small pieces, add deodorizer, soak for 10-15 hours, and drain;

[0021] 2) Degreasing: Homogenize the sea cucumber after deodorization, add degreasing agent, reflux extraction, degreasing agent is acetone-ethyl acetate mixed solution, acetone:ethyl acetate volume ratio is 1:2~4, homogenate liquid: mixed liquid The volume ratio is 1:20~25, the reflux temperature is 70~80°C, the reflux time is 50~60min, and the degreasing is repeated 1~3 times. Dry to obtain defatted sea cucumber powder. Fat is oxidized during the enzymatic hydrolysis process, which will lead to changes in the color and smell of the enzymatic hydrolysis solution; secondly, the emulsification of fat and protein will make it difficult to separate the hydrolyzed solution; therefore, fat treatment is an important condition to ensure the smooth preparation of protein . The above-mentioned...

Embodiment 2

[0028] A method for extracting sea cucumber collagen, the most preferred steps are:

[0029] 1) Deodorization: Weigh 10kg sea cucumbers, wash them with clean water, cut the sea cucumbers into small pieces of 1cm×1cm×1cm, add the deodorizing agent, immerse the small pieces of sea cucumbers, the components of the deodorization liquid and the most preferred parts by weight are : 18 parts perilla extract, 12 parts ginger extract, 7 parts garlic extract, 3 parts ethanol, 6 parts cinnamon stick extract, 4 parts soft-shelled turtle bile extract. The fishy smell removal liquid can not only react with trimethylamine to produce quaternary ammonium salt, but also can dissolve trimethylamine, has high solubility, short soaking time, and remarkable fishy smell removal effect. Soaking, soaking time is 15h, drain;

[0030] 2) Degreasing: Homogenize the sea cucumber after deodorization, add degreasing agent, reflux extraction, degreasing agent is acetone-ethyl acetate mixture, acetone: ethyl...

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Abstract

The invention provides an extraction method of sea cucumber collagen. The extraction method comprises the operation steps of (1) deodorization, (2) degreasing; (3) stepwise enzymolysis, (4) alcohol precipitation and (5) ultra-filtration and drying. The method has the beneficial effects that enzymolysis is carried out on endonuclease papain and chymotrypsin, the exposure rate of amino acid restriction enzyme sites of the sea cucumber collagen is increased, enzymolysis is carried out on exonuclease flavourzyme, full enzymolysis is carried out on the sea cucumber collagen, and the amount of the sea cucumber collagen is increased, so that the yield is improved; the finally obtained sea cucumber collagen is high in purity and small in molecular weight, can directly reach a skin corium layer, the moisture is locked, the elasticity of the skin is increased, wrinkles are smoothed, the sea cucumber collagen is high in activity and high in antioxidant ability, in vivo free radicals are removed and the sea cucumber collagen plays an anti-aging role. The method is simple in operation step, the sea cucumber collagen can be obtained, a lot of by-products with high economic value can be obtained, the added economic value of sea cucumber is increased and a new way is added for treatment of the sea cucumber.

Description

technical field [0001] The invention relates to the technical field of protein extraction, in particular to a method for extracting sea cucumber collagen. Background technique [0002] Sea cucumber is an echinoderm, an ancient marine mollusk. With a survival history of more than 600 million years, it is famous for its "warm and nourishing nature, which is enough to rival wild ginseng". It ranks first among the "Eight Treasures of Seafood". It is expensive and has a long-standing reputation as a nourishing treasure. The nutritional value of sea cucumber is extremely high, rich in polyglucosamine, mucopolysaccharide, marine bioactive calcium, high protein, mucin, polypeptide, collagen, nucleic acid, sea cucumber saponin, chondroitin sulfate, multivitamins, and various amino acids and More than 50 kinds of nutrients such as carbohydrates are rare high-grade tonics without cholesterol. [0003] Sea cucumbers are rich in polyglucosamine, bioactive calcium, sea cucumber mucopoly...

Claims

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Application Information

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IPC IPC(8): A23J1/04
CPCA23J1/04
Inventor 施春华张美芬
Owner 嵊州市派特普科技开发有限公司
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