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Photoelectric immune sensor based on water-soluble Cd-Ag-Te quantum dot/nano-gold composite materials

A composite material and nano-gold technology, which is applied in the preparation of new water-soluble Cd-Ag-Te quantum dots/nano-gold composite materials, and the application field of cardiac troponin I photoelectric immunosensor, which can solve the problem of low photoelectric conversion efficiency, etc. problems, to achieve the effect of maintaining antibody biological activity, strong fluorescence intensity and stability, and good conductivity

Inactive Publication Date: 2017-05-10
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Quantum dots are an important semiconductor optoelectronic material, widely used in photoelectrochemical sensing research, but the photoelectric conversion efficiency of a single quantum dot material is low due to the recombination of internal electron-hole pairs

Method used

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  • Photoelectric immune sensor based on water-soluble Cd-Ag-Te quantum dot/nano-gold composite materials
  • Photoelectric immune sensor based on water-soluble Cd-Ag-Te quantum dot/nano-gold composite materials
  • Photoelectric immune sensor based on water-soluble Cd-Ag-Te quantum dot/nano-gold composite materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A novel water-soluble Cd-Ag-Te quantum dot, its synthesis steps are as follows:

[0054] Preparation of NaHTe: add 0.45g NaBH to a glass bottle 4 , 10mL ultrapure water, ice bath, add 0.0127g tellurium powder, sealed reaction 2h (equation: 2Te+4NaBH 4 +7H 2 O→2NaHTe+Na 2 B 4 o 7 +14H 2 ↑), to obtain a colorless and transparent NaHTe solution (concentration is 0.01mol L -1 ),spare.

[0055] Scheme 1: Under nitrogen protection, add 80mL of 0.002mol·L to a 250mL three-neck round bottom flask -1 CdCl 2 Solution, 20mL 0.016mol·L -1 N-acetyl-L-cysteine ​​solution, 40ml twice distilled water, mix the solution evenly; use 1mol L -1 NaOH solution to adjust the pH of the mixture to 11.5; add 2mL of 0.01mol L -1 AgNO 3 solution, and react in the dark for 1h; then add 3mL of freshly prepared 0.01mol·L -1 NaHTe solution, under the protection of nitrogen, reacted at 100°C for 1h, and obtained the fluorescence emission wavelength of 550nm, such as figure 1 Cd-Ag-Te quant...

Embodiment 2

[0061] A preparation method of nano gold, the steps are as follows:

[0062] (1) Synthetic gold species: add 0.42mL 0.002mol L to 0.951mL twice distilled water -1 HAuCl 4 , mix well, add 1.25mL 0.2mol·L -1 1-(10-Bromodecyl)-3-methylimidazolium bromide, then add 0.3mL of freshly prepared 0.01mol·L -1 NaBH 4 Solution, standing at room temperature for 2h to obtain nano-gold species;

[0063] (2) Synthesis of nano-gold: 2.6mL twice distilled water, 1.67mL 2×10 -3 mol L - 1 HAuCl 4 , 3.96mL 0.4mol L -1 1-(10-Bromodecyl)-3-methylimidazolium bromide, 54 μL 0.1mol L -1 Ascorbic acid was stirred vigorously for 2 minutes, and finally 80 μL of the above-prepared gold species was added, stirred for 20 seconds, and left at room temperature for 24 hours to prepare nano-gold.

[0064] UV-Vis absorption spectra such as Figure 4 As shown, the maximum absorption peak of nano-gold is at 540nm, which is obviously related to figure 1 The Cd-Ag-Te quantum dots shown in a have the bes...

Embodiment 3

[0066] A kind of preparation method of water-soluble Cd-Ag-Te quantum dot / nano gold composite material, its steps are as follows:

[0067] Add 16 μL 0.01mol·L -1 Mercaptoethylamine solution, mixed evenly, and allowed to stand at room temperature for 12 hours; 100 μL of quantum dot solution containing 10 mg·mL -1 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 20mg·mL -1 A mixed solution of N-hydroxysuccinimide (NHS), mixed evenly, and left to stand at 37°C for 1h; the above two solutions were mixed evenly, let stand at 37°C for 24h, centrifuged and washed 3 times with twice distilled water, and washed with two Diluted to 1 mL with sub-distilled water to obtain a Cd-Ag-Te quantum dot / nano-gold composite material, referred to as Cd-Ag-Te / AuNPs composite material.

[0068] through transmission electron microscopy Figure 6 As shown, the gold nanoparticles agglomerated to a certain extent, and a large number of quantum dots were closely attached to the su...

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Abstract

The invention belongs to the field of disease marker detection, photoelectron chemistry and immune sensor technique, and particularly discloses a preparation method of novel water-soluble Cd-Ag-Te quantum dot / nano-gold composite materials. According to the method, L-shaped glassy carbon electrodes are decorated, anti-cTnI (cardiac troponin I antibodies) are loaded, so that photoelectric immune sensors are established based on the novel water-soluble Cd-Ag-Te quantum dot / nano-gold composite materials, an LED (light-emitting diode) serves as an excitation light source, and cTnI (cardiac troponin I) specificity is detected based on immune reaction. According to the preparation method, water-soluble Cd-Ag-Te quantum dots, nano-gold and 405nm LED excitation light sources are introduced into cTnI photoelectric immune sensor systems, and the prepared photoelectric immune sensor is simple, economical, practical, simple and convenient to operate, good in selectivity, high in sensitivity and low in detection limit.

Description

technical field [0001] The invention also relates to the technical fields of disease marker detection, photoelectrochemistry, and immune sensing, and specifically relates to the preparation and characterization of a new water-soluble Cd-Ag-Te quantum dot / nano-gold composite material and the energy between quantum dots and nano-gold Application of transfer in a photoimmunosensor for cardiac troponin I. Background technique [0002] Photoelectrochemical sensor is a sensing device that uses the photoelectric properties of substances to detect analytes, and is an analytical method developed in recent years. As a means of analysis, photoelectrochemistry is just the opposite of electrochemiluminescence in its detection process. Due to the use of different forms of excitation and detection signals, the background signal is low, and high sensitivity comparable to electrochemiluminescence may be achieved. In addition, photoelectrochemical instruments are relatively simple and easy ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N27/327
CPCG01N27/3275G01N33/6887
Inventor 李春涯谭媛王炎英利明师
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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