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Photoelectric-chemical-sensor-based method for detecting organophosphorus residues

An organophosphorus and carboxyl group technology is applied to the preparation of water-soluble amino cadmium telluride/carboxy cadmium telluride quantum dot composite materials, and the preparation of organophosphorus hydrolase photoelectrochemical sensors, which can solve the problems of low photoelectric efficiency and the like, and achieve the The method is simple, the photocurrent is stable, and the time required for detection is saved.

Inactive Publication Date: 2019-05-24
武汉中科志康生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Quantum dots are an important semiconductor optoelectronic material, widely used in photoelectrochemical sensing research, but the photoelectric efficiency of a single quantum dot material is low due to the recombination of internal electron-hole pairs

Method used

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  • Photoelectric-chemical-sensor-based method for detecting organophosphorus residues
  • Photoelectric-chemical-sensor-based method for detecting organophosphorus residues
  • Photoelectric-chemical-sensor-based method for detecting organophosphorus residues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A water-soluble carboxyl cadmium telluride quantum dot (CdTe-COOH QDs) solution, the synthesis steps are as follows:

[0035] Under nitrogen protection, add 0.6 mmol CdCl to a 250 mL three-neck round bottom flask successively 2 , 1 mmol 3-mercaptopropionic acid and 100 mL double-distilled water, and magnetically stirred to mix evenly to obtain a mixed solution; use 1.0 mol·L -1 NaOH solution to adjust the pH value of the mixed solution to 11.5; add 120 mg NaBH 4 , reacted for 1h; then added 0.06 mmol Na 2 TeO 3 , N 2 React under protection for 6 h. Cool the solution obtained from the reaction to room temperature, centrifuge with a 5kD ultrafiltration tube (to concentrate and purify the quantum dot solution) to obtain a water-soluble CdTe-COOH quantum dot solution with an average diameter of 4-5nm, and store it at 4°C for later use.

Embodiment 2

[0037] A water-soluble amino cadmium telluride quantum dot (CdTe-NH 2 QDs) solution, the synthesis steps are as follows:

[0038] Under nitrogen protection, add 100 mL of 12 mmol·L to a 250 mL three-neck round bottom flask successively -1 CdCl2 solution, 3.6 mmol mercaptoethylamine, magnetically stirred and mixed uniformly to obtain a mixed solution; add 100 mg NaBH 4 , reacted for 1h; then added 0.06 mmol Na 2 TeO 3 , N 2 React under protection for 1 h. Cool the solution obtained from the reaction to room temperature, and centrifuge with a 5kD ultrafiltration tube (to concentrate and purify the quantum dot solution) to obtain water-soluble CdTe-NH 2 Quantum dot solution, the average diameter of quantum dots is 2-3nm, stored at 4°C for future use.

Embodiment 3

[0040] A CdTe-NH 2 / CdTe-COOH QDs composite material, the synthesis steps are as follows:

[0041] In the CdTe-COOH quantum dot solution that 0.1mL embodiment 1 obtains, add 0.1mL containing 10mg·mL -1 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 20mg•mL -1 Mix the N-hydroxysuccinimide evenly, and let it stand at 37° C. for 1 h to obtain solution I; mix the solution I with 0.6 mL of the CdTe-NH obtained in Example 2 2 The QDs solution was mixed evenly, and stood at 37°C for 20 hours to obtain solution II; the solution II was centrifuged and washed 3 times, and diluted to 2 mL with twice distilled water to obtain CdTe-NH 2 / CdTe-COOH QDs composites.

[0042] Regarding this step, the CdTe-NH 2 The optimal volume of QDs solution can be determined by examining the photocurrent response of different sensors and the CdTe-NH 2 The relationship curve of the volume of QDs solution is determined: in the range of 0.1-0.6mL, with the CdTe-NH 2 As the volume o...

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Abstract

The invention relates to an photoelectric-chemical-sensor-based method for detecting organophosphorus residues and belongs to the technical field of photoelectrochemistry and pesticide residue detection. The method has the advantages that a water-soluble amino cadmium telluride / carboxyl cadmium telluride quantum dot composite material is prepared, and an L-shaped glassy carbon electrode modified by the composite material and loaded with organophosphorus hydrolase is used to build a photoelectric sensor; the organophosphorus hydrolase can catalyze organophosphorus pesticides to generate groupswith electrochemical activity, the groups have redox reaction on the surface of the electrode to generate electrons, and the electrons and the photoelectric composite material have hole recombinationunder an illumination condition so as to detect organophosphorus pesticides. The organophosphorus hydrolase photoelectric sensor prepared by the method is simple to prepare, economical and practical,simple to operate and high in in selectivity and sensitivity, and the organophosphorus pesticide detection limit of the sensor reaches 0.7pg*mL<-1>.

Description

technical field [0001] The invention belongs to the technical field of photoelectrochemistry and pesticide residue detection, and relates to the preparation of a water-soluble amino cadmium telluride / carboxycadmium telluride quantum dot composite material, the preparation of an organophosphorus hydrolase photoelectrochemical sensor based on the photoelectric composite material and the The photoelectric sensor is a method for detecting organophosphorus pesticides. Background technique [0002] At present, the use of pesticides, especially organophosphorus pesticides, is the main measure to prevent and control crop pests and diseases. They have the advantages of high toxicity and long persistence. However, many pesticides and their degradation products will remain in various foods, ecological environments and drinking water. , which has also brought serious food safety and environmental pollution problems. Conventional pesticide residue detection techniques such as gas chroma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 吴莹莹
Owner 武汉中科志康生物科技有限公司
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