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Oligographic bacterium composition and application of oligographic bacterium composition in inhibition on generation of jarosite in biological leaching

An oligotrophic and bacterial technology, applied in the field of microorganisms, can solve problems such as difficult removal, achieve the effect of improving bioleaching efficiency and solving difficult bioleaching

Inactive Publication Date: 2017-05-10
GRIMAT ENG INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that once jarosite is generated in the leaching system, it is difficult to remove

Method used

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  • Oligographic bacterium composition and application of oligographic bacterium composition in inhibition on generation of jarosite in biological leaching
  • Oligographic bacterium composition and application of oligographic bacterium composition in inhibition on generation of jarosite in biological leaching
  • Oligographic bacterium composition and application of oligographic bacterium composition in inhibition on generation of jarosite in biological leaching

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the acquisition of oligotrophic bacteria

[0038] Step 1. Enrichment

[0039] The water samples containing oligotrophic bacteria obtained in the present invention are collected from mine water collected in Dexing Copper Mine, Jiangxi. According to the proportion of 0.3g yeast powder added to 100ml water sample, three groups were cultured in parallel at different temperatures (30°C, 45°C, 55°C), and cultured with gentle shaking at 100rpm for 1 week. Microscopic examination was used to judge the growth of bacteria, and it was found that the growth of bacteria was the best at 45°C, and the culture solution of oligotrophic bacteria was collected at 45°C.

[0040] Step 2, culture medium preparation

[0041] The basic preparation method of the separation medium is: weigh 0.25g yeast powder, 0.5g peptone, 0.25g NaCl and 1000mL distilled water, mix well and add 30g / L gellan gum. Prepare 100mL culture medium in proportion, adjust the pH to 3.0, sterilize at 121°...

Embodiment 2

[0050] Example 2: Determination of the phylogenetic position of the oligotrophic bacteria Biometek-A, Biometek-B and Biometek-AP-AC

[0051] Step 1. DNA extraction

[0052] Use the following methods to extract Biometek-A, Biometek-B and Biometek-AP-AC respectively to obtain the total DNA of the corresponding bacteria:

[0053] Under sterile conditions, take 1.5ml of bacterial liquid and add it to an Eppendorf centrifuge tube; centrifuge at 4°C and 5000rpm for 5 minutes to collect the bacteria; suspend the bacteria in 1×TES liquid, centrifuge at 5000rpm for 5 minutes, and wash the bacteria twice; Re-suspend the bacteria in 435μl of TES solution, and fully break up the bacteria; add 125ul of lysozyme (20mg / ml), 2.5μl of proteinase K (10mg / ml), shake gently in a 37°C water bath for 2 hours, add 50μl 20% SDS, gently shake and incubate in a 37°C water bath until the solution is completely clear; add 5M NaClO 4 125 μl, extract with equal volume of phenol: chloroform: isoamyl alco...

Embodiment 3

[0074] Example 3: The effect of oligotrophic bacteria combination on the production of jarosite in the chalcopyrite bioleaching system.

[0075]The oligotrophic bacteria combination is to inoculate the identified and preserved Biometek-A, Biometek-B and Biometek-AP-AC strains into the 200mL enrichment medium prepared in step 2, and cultivate them on a shaker at 45°C at 100rpm for one week to obtain three kinds of bacteria culture medium. Concentrate the culture solution into bacteria sludge according to the conventional method, and then mix the three kinds of bacteria according to the ratio of 1:1:1 according to the amount of bacteria.

[0076] The preparation method of autotrophic bacterium Acidithiobacillus caldus OY is: autotrophic bacterium Acidithiobacillus caldus OY is inoculated into 200mL 9K medium (9K medium formula: (NH 4 ) 2 SO 4 3.0g; KCl 0.1g; K 2 HPO 4 0.5g; MgSO 4 ·7H 2 O 0.5g; Ca(NO 3 ) 2 0.01g; FeSO 4 ·7H 2 O 44.22g; distilled water 1000.0ml; pH ...

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Abstract

The invention provides an oligographic bacterium composition. The strain category name of the oligographic bacterium composition is alicyclobacillus sp., and the strain codes are as follows: Biometek-A, Biometek-B and Biometek-AP-AC. The Depository Authority is China General Microbiological Culture Collection Center (CGMCC) and the address is Institute of Microbiology of Chinese Academy of Sciences, 3#, No.1 Yard, West Beichen Road, Chaoyang District, Beijing; the preservation date is December 26, 2012 and the preservation numbers are as follows: CGMCC No.7038, CGMCC No.7039 and CGMCC No.7040; and the bacterium composition is formed by mixing the strains in the ratio of 1 to 1 to 1. According to the composition provided by the invention, an additional carbon source can be utilized for reducing ferric ions in a system, so that the generation of jarosite is inhibited.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an oligotrophic bacterial combination capable of inhibiting jarosite formation in a bioleaching process. Background technique [0002] According to existing research, the reason why chalcopyrite is difficult to be used in bioleaching is not only its high lattice energy, but more importantly, a dense passivation film will be formed on the surface of the mineral during the leaching process, thereby hindering the leaching of nutrients. , the transfer of microorganisms and reaction products between the mineral surface and the solution, and inhibit the continuous leaching of copper. The composition and formation mechanism of this passivation film are closely related to the adsorbed microorganisms on the mineral surface, extracellular polymers, mineral decomposition products and products generated in the solution. It is generally believed that after ore leaching microorganisms ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C22B3/18C12R1/01
CPCY02P10/20
Inventor 刘文彦孟春瑜刘兴宇陈勃伟
Owner GRIMAT ENG INST CO LTD
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