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Preparation method and application of sleeve-fish collagen polypeptide and iron complex thereof

A technology of collagen peptides and iron complexes, applied in the field of medicine, to achieve the effects of easy control, no pollutant discharge, and content reduction

Active Publication Date: 2017-05-10
海之贻生物科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using modern biotechnology to extract collagen peptides, improve biological activity, and further develop raw materials for medicine, health care and beauty cosmetics, there have been no reports at home and abroad.

Method used

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  • Preparation method and application of sleeve-fish collagen polypeptide and iron complex thereof
  • Preparation method and application of sleeve-fish collagen polypeptide and iron complex thereof
  • Preparation method and application of sleeve-fish collagen polypeptide and iron complex thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1. Preparation of squid skin collagen polypeptide

[0025] 200Kg of fresh squid skin, minced with a meat grinder. The shredded squid skin is placed in a high-pressure reaction pot. Add 1200L of 3% acetic acid water (i.e. water 1160L, glacial acetic acid 36Kg or 72 bottles of 500ml glacial acetic acid mixed), and stir while adding until the addition is complete. After the acetic acid water is added and stirred evenly, start heating and control the temperature at 121°C. After hydrolysis for 1 hour, stop heating immediately, let it cool down to ~105°C naturally, then slowly open the release valve, open the feeding port, and add 2.5Kg Activated carbon, keep warm at 90-100°C for 20 minutes. Then centrifuge, and the centrifugate is concentrated to about 300L. After the concentrate was cooled to room temperature, 3 times the amount (V / V) of 95% ethanol was added, stirred while adding, and allowed to settle overnight. The precipitate was centrifuged, and the filt...

Embodiment 2

[0027] Embodiment 2. Preparation of squid skin collagen polypeptide

[0028] Fresh squid skin 100Kg, minced with a meat grinder. The shredded squid skin is placed in a high-pressure reaction pot. Add 600L of 8% acetic acid water (that is, 580L of water and 18Kg of glacial acetic acid mixed), and stir while adding until the addition is complete. After the acetic acid water is added and stirred evenly, start heating, the temperature is controlled at 110°C, after hydrolysis for 1 hour, stop heating immediately, let it cool down to ~105°C naturally, then slowly open the release valve, open the feeding port, and add 1Kg of activated carbon , 90 ~ 100 ℃ for 20 minutes. Then centrifuge, and the centrifugate is concentrated to about 150L. After the concentrate was cooled to room temperature, 3 times the amount (V / V) of 95% ethanol was added, stirred while adding, and allowed to settle overnight. The precipitate was centrifuged, and the filtrate was filtered again. The ethanol was...

Embodiment 3

[0029] Embodiment 3. Preparation of squid collagen polypeptide iron complex

[0030] Dissolve 30.00 g of squid skin hydrolyzed peptide in 120 mL of distilled water, add 2.8% ferrous sulfate and dissolve in 30 mL. Under magnetic stirring, the ferrous sulfate solution was slowly added to the squid skin hydrolyzed peptide solution, and reacted at room temperature for 3 hours. After the reaction solution was filtered with absorbent cotton, 3 times the amount of 95% ethanol was added to the filtrate for precipitation. Suction filtration, washing with absolute ethanol twice, and the filter residue was vacuum-dried at room temperature (removing ethanol). Reuse 420mL of distilled water to dissolve and freeze-dry to obtain squid skin hydrolyzed peptide ferrous salt (loose brown-yellow scales). The yield was 47.8%.

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Abstract

The invention discloses a preparation method and application of sleeve-fish collagen polypeptide and an iron complex thereof. A high-temperature acetic acid hydrolysis method is utilized to prepare the sleeve-fish collagen polypeptide and the iron complex thereof from sleeve-fish skin which is a byproduct of sleeve-fish processing, and the preparation method is stable in reaction condition, easy to control and free of pollutant emission and meets requirements on clean production. Animal experiments show that the sleeve-fish collagen polypeptide and the iron complex thereof can remarkably increase hypoxia tolerance survival time, improve activity of in-vivo lactic dehydrogenase and reduce content of in-vivo blood lactic acid and blood urea nitrogen. Results show that hypoxia tolerance activity can be remarkably improved by adopting either the sleeve-fish collagen polypeptide or the iron complex, and the sleeve-fish collagen polypeptide and the iron complex can be used for preparing hypoxia-tolerant drug and functional food and are of important significance in developing and utilizing marine medicinal biological resources in China.

Description

technical field [0001] The invention belongs to the technical field of medicine, and more specifically relates to the preparation method and application of squid collagen polypeptide and iron complex thereof. Background technique [0002] Squid (Loligo chinensis) is a marine organism belonging to the mollusk phylum Squid. It is widely distributed in the South my country Sea and Southeast Asia waters of China. It is rich in nutrition and high in protein content, and can be processed into a variety of delicious foods. During squid processing, the outer skin is removed, and the squid skin is often used as waste. Researches at home and abroad have found that fish processing wastes account for about 30% of the total, which is rich in protein resources, mainly collagen, and its hydrolyzed products contain a variety of active peptides. Active peptides have physiological and pharmacological activities such as anti-tumor, anti-ulcer, lowering blood pressure, promoting bone formati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/12C07K1/107A61K38/01A61P39/00A23L33/18A23L33/165A23J1/04
CPCA23J1/04A23V2002/00A61K38/00C07K14/78A23V2250/1592A23V2250/55
Inventor 易杨华
Owner 海之贻生物科技(上海)有限公司
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