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A method for separation and extraction of chitotetraose monomer based on molecular imprinting technology

A molecular imprinting technology, molecular imprinting technology, applied in the direction of amino sugar, chemical instruments and methods, sugar derivatives, etc., can solve the problems of low purity, high price, and separation research has not yet been published, and achieve simple separation and extraction process and market competition The effect of strong force and high product purity

Active Publication Date: 2019-03-26
郑永军 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the separation and preparation of marine chitooligosaccharide monomers have the following problems: (1) low purity
Due to the complex separation and purification process of chitosan oligosaccharides, it is difficult to obtain high-purity chitosan oligosaccharide monomers by conventional separation methods such as step-by-step sedimentation and membrane separation.
Therefore, most of the currently commercially available marine oligochitosan products are low-polymerization oligochitosan mixtures, which restricts the high-value development and utilization of oligochitosan
(2) Small output
Currently used gel chromatography, ion exchange chromatography, polyacrylamide gel electrophoresis, cellulose acetate membrane electrophoresis, high performance liquid chromatography and high performance capillary electrophoresis, rapid preparative liquid chromatography and perfusion chromatography and other instruments The separation method can only prepare a small amount of chitosan oligosaccharide monomer (usually at the mg level), and it is difficult to realize the large-scale separation and preparation of chitosan oligosaccharide monomer
(3) Expensive
Looking at the research status at home and abroad, most of the template molecules are monosaccharides and disaccharides below trisaccharides (such as raffinose), and there are few reports on the separation and identification of oligosaccharides above trisaccharides. Separation studies have not yet been published

Method used

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  • A method for separation and extraction of chitotetraose monomer based on molecular imprinting technology
  • A method for separation and extraction of chitotetraose monomer based on molecular imprinting technology
  • A method for separation and extraction of chitotetraose monomer based on molecular imprinting technology

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Preparation: Take 65kg ethylene glycol dimethacrylate (EGDMA); 5kg N-[2-(dimethylamino)ethyl]-N'-[(2-hydroxy-4-vinylphenyl)]oxamide Binuclear zinc-chitotetraose; 5kg petroleum ether 90-120; 0.5kg polyvinyl alcohol (PVA); 0.05kg azobisisobutyronitrile (AIBN);

[0028] Preparation of chitotetraose molecularly imprinted resin: N-[2-(dimethylamino)ethyl]-N'-[(2-hydroxyl-4-vinylphenyl)] oxamide dinuclear zinc-chitotetraose and even Azodiisobutyronitrile is dissolved in absolute ethanol according to the proportion by weight, so that N-[2-(dimethylamino)ethyl]-N'-[(2-hydroxyl-4-vinylphenyl)] grass The mass percent concentration of amide dinuclear zinc-chitotetraose and azobisisobutyronitrile in dehydrated alcohol is 10%, then the mixed solution is mixed with ethylene glycol dimethacrylate, petroleum ether 90-120; Under strong stirring, slowly add this mixed system into a three-necked flask containing polyvinyl alcohol, pass through nitrogen to remove oxygen for 30 minutes; he...

Embodiment 2

[0037] Preparation: Take 80kg ethylene glycol dimethacrylate (EGDMA); 10kg N-[2-(dimethylamino)ethyl]-N'-[(2-hydroxy-4-vinylphenyl)]oxamide Binuclear zinc-chitotetraose; 7kg petroleum ether 90-120; 1kg polyvinyl alcohol (PVA); 0.15kg azobisisobutyronitrile (AIBN);

[0038] Preparation of chitotetraose molecularly imprinted resin: N-[2-(dimethylamino)ethyl]-N'-[(2-hydroxyl-4-vinylphenyl)] oxamide dinuclear zinc-chitotetraose and even Azodiisobutyronitrile is dissolved in absolute ethanol according to the proportion by weight, so that N-[2-(dimethylamino)ethyl]-N'-[(2-hydroxyl-4-vinylphenyl)] grass The mass percent concentration of amide dinuclear zinc-chitotetraose and azobisisobutyronitrile in dehydrated alcohol is 15%, then the mixed solution is mixed with ethylene glycol dimethacrylate, petroleum ether 90-120; Under strong stirring, slowly add this mixed system into a three-necked flask containing polyvinyl alcohol, pass through nitrogen to remove oxygen for 30 minutes; hea...

Embodiment 3

[0047] Preparation: Take 95kg ethylene glycol dimethacrylate (EGDMA); 15kg N-[2-(dimethylamino)ethyl]-N'-[(2-hydroxy-4-vinylphenyl)]oxamide Binuclear zinc-chitotetraose; 9kg petroleum ether 90-120; 1.5kg polyvinyl alcohol (PVA); 0.3kg azobisisobutyronitrile (AIBN);

[0048] Preparation of chitotetraose molecularly imprinted resin: N-[2-(dimethylamino)ethyl]-N'-[(2-hydroxyl-4-vinylphenyl)] oxamide dinuclear zinc-chitotetraose and even Azodiisobutyronitrile is dissolved in absolute ethanol according to the proportion by weight, so that N-[2-(dimethylamino)ethyl]-N'-[(2-hydroxyl-4-vinylphenyl)] grass The mass percent concentration of amide dinuclear zinc-chitotetraose and azobisisobutyronitrile in dehydrated alcohol is 20%, then the mixed solution is mixed with ethylene glycol dimethacrylate, petroleum ether 90-120; Under strong stirring, slowly add this mixed system into a three-necked flask containing polyvinyl alcohol, pass through nitrogen to remove oxygen for 30 minutes; he...

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Abstract

The invention discloses a method for separating and extracting a chitotetraose hydrochloride monomer based on molecular imprinting technology. The method comprises the following steps: cloth bag filtration, nanofiltration membrane filtration, adsorption through a chitotetraose hydrochloride molecular imprinting resin, elution of the chitotetraose hydrochloride molecular imprinting resin, removal of zinc ions and freeze drying. Compared with a method in the prior art, the method is simple in separation technical process, low in production cost, and high in purity of chitotetraose hydrochloride monomer and allows massive preparation to be achieved.

Description

technical field [0001] The invention relates to the research field of separation and purification of marine chitosan oligosaccharide monomers, in particular to a method for separation and extraction of chitosan monomers based on molecular imprinting technology. Background technique [0002] Oligosaccharides and glycoconjugates are important information substances in organisms, and play an extremely important role in cell recognition, signal transduction and receptor regulation in life processes. Chitooligosaccharides (COS) are products obtained after deacetylation and degradation of marine chitin, generally composed of 3-10 glucosamine linked by β-1,4-glycosidic bonds. Oligochitosan has low molecular weight, can be dissolved in water, and has better biological activity than chitosan. It is widely used in the fields of health care products, biochemical materials, and food. At present, the separation and preparation of marine chitooligosaccharide monomers have the following p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H5/06C07H1/06
CPCC07H1/06C07H5/06
Inventor 郑永军郑康
Owner 郑永军
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