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Application of alpha-lactose in preparation of drugs for treatment of sepsis

A sepsis and lactose technology, applied in the field of biomedicine, can solve the problems of exacerbation of sepsis immunosuppression, plight of sepsis immunotherapy, correction of unfavorable immune disorders, etc., to reduce immune disorders and reduce multiple organ dysfunction risk, and the effect of improving immune disorders

Inactive Publication Date: 2017-04-26
唐朝晖
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] 2. The Dilemma of Sepsis Immunotherapy
In addition, we found that the loss of Tim-3 function can significantly improve the early survival rate (28 days) still did not improve
These previous studies suggest that Tim-3 signaling is closely related to immune disorders after sepsis. High expression of Tim-3 induces excessive inflammatory responses that are harmful to the body, but the lack of Tim-3 is also not conducive to the correction of immune disorders
[0013] 4. α-lactose interferes with the effect of Tim-3 / Galectin-9 signaling pathway
At present, there is no report on the use of α-lactose alone as a clinical agent to treat diseases, let alone the application of α-lactose to treat sepsis

Method used

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  • Application of alpha-lactose in preparation of drugs for treatment of sepsis
  • Application of alpha-lactose in preparation of drugs for treatment of sepsis
  • Application of alpha-lactose in preparation of drugs for treatment of sepsis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Observation of the effect of α-lactose treatment on the survival rate of septic mice.

[0046] Preparation of sepsis mouse model:

[0047] The mouse model of sepsis was prepared by the classic cecal ligation and puncture (CLP) method. For detailed methods, see references 15 , see the attached figure 1 . This model simulates sepsis caused by multiple bacterial infections in humans. The sham operation group was used as the control. The 72-hour mortality of sepsis mice was about 70%, and the 72-hour mortality of mice in the sham operation group was less than 5%.

[0048] 1.2 Effect of treatment with α-lactose on the survival rate of septic mice:

[0049] C57BL / 6 mice, male, aged 8 weeks, weighing 20-24 g. The sepsis mouse model was prepared by mouse cecal ligation and puncture (CLP). After the model was successfully established, all mice were randomly divided into lactose group, sucrose group (similar to lactose, which is also a disaccharide) and contro...

Embodiment 2

[0052] Example 2: The effect of α-lactose treatment on the immune disorder of septic mice was observed.

[0053] The cascade release of inflammatory factors after sepsis and the massive apoptosis of immune cells lead to a sharp decrease in the ratio and number of immune cell subsets are two prominent features of immune dysfunction after sepsis. This part of the experiment mainly observes the effect of treatment with α-lactose on the immune disorder of septic mice.

[0054] Preparation and treatment of sepsis mouse model

[0055] C57BL / 6 mice, male, aged 8 weeks, weighing 20-24g. The mouse model of sepsis was prepared by cecal ligation and puncture (CLP), and the method was the same as that in 1.1 above. After successful modeling, all mice were randomly divided into treatment group and control group. Lactose group: 0, 2, 6, 18, and 36 hours after the establishment of the sepsis mice, 200ul of α-lactose solution with a concentration of 10% prepared by 0.9% normal saline was s...

Embodiment 3

[0066] Example 3: Observation of the effect of α-lactose treatment on liver function damage in septic mice.

[0067] Sepsis can easily induce the dysfunction of important organs, especially the dysfunction of the liver, and the liver plays a central role in the body's metabolic regulation, self-defense, and bacterial clearance. Liver dysfunction after sepsis is a significant independent predictor of poor patient outcome. This part of the experiment mainly observed the effect of α-lactose treatment on liver damage in septic mice.

[0068] Preparation and treatment of sepsis mouse model

[0069] C57BL / 6 mice, male, aged 8 weeks, weighing 20-24g. The mouse model of sepsis was prepared by cecal ligation and puncture (CLP), and the method was the same as that in 1.1 above. After successful modeling, all mice were randomly divided into treatment group and control group. Lactose group: 0, 2, 6, 18, and 36 hours after the establishment of the sepsis mice, 200ul of α-lactose soluti...

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Abstract

The invention discloses a method for treatment of sepsis. Specifically, multiple aspects of the invention relate to application of alpha-lactose in preparation of drugs for treatment of sepsis. Alpha-lactose is a disaccharide, and is an anomer. Research shows that subcutaneous injection of an alpha-lactose solution can significantly improve the 28d survival rate of a sepsis model mouse, can significantly improve release of inflammatory factors in a waterfall mode after sepsis and immune dysfunction caused by heavy immune cell apoptosis, and can alleviate liver injury induced by sepsis. Alpha-lactose is a pharmaceutic adjuvant included in the "Pharmacopoeia of the People's Republic of China", is widely used as a flavoring agent or filler, and has the advantages of low price, clear physicochemical properties and toxicity, easy dissolution in water, intravenous injection, and slight side effect. Under the current situation of high incidence and high mortality of sepsis patients, and lack of effective therapeutic drugs, alpha-lactose has enormous application value and prospects in preparation of drugs for treatment of sepsis.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to the application of alpha-lactose in the preparation of medicines for treating sepsis. Background technique [0002] Sepsis is a systemic inflammatory response syndrome induced by severe trauma, burns, shock, infection and other critical illnesses, and is one of the most common causes of death in clinical critically ill patients. In recent years, the incidence of sepsis has been increasing and it has become a major public health problem 1 . According to statistics in the United States, 3 out of every 1,000 hospitalized patients suffer from sepsis, and this number is increasing at a rate of 2-8% per year. 2 . According to incomplete statistics, there are more than 3 million new cases of sepsis every year in my country. 3 . Although a variety of comprehensive treatment measures such as anti-infection, fluid resuscitation and related organ function support have been taken, th...

Claims

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Application Information

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IPC IPC(8): A61K31/7016A61P31/00
CPCA61K31/7016
Inventor 唐朝晖余彦唐庭轩
Owner 唐朝晖
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