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Latex immunoturbidimetric kit for detecting AGEs

A detection reagent and reagent technology, applied in the field of in vitro diagnostic reagents, can solve the problems of low degree of automation, cumbersome operation process, long consumption time, etc., and achieve the effect of improving linearity and sensitivity, convenient use and good stability

Active Publication Date: 2017-04-19
王贤俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, its detection methods include radioreceptor analysis, radioimmunoassay, enzyme-linked immunosorbent assay, high-performance liquid chromatography, etc., but these methods are cumbersome to operate, with large errors, long time consumption, low degree of automation, and high cost. Low sensitivity, very unfavorable for clinical, generally only used for qualitative detection

Method used

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  • Latex immunoturbidimetric kit for detecting AGEs
  • Latex immunoturbidimetric kit for detecting AGEs
  • Latex immunoturbidimetric kit for detecting AGEs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] AGEs detection reagents, including reagent R1 and reagent R2:

[0021] Reagent R1 contains

[0022]

[0023]

[0024] Reagent R2 contains

[0025]

[0026] The AGEs detection reagent described in this example uses a dual-reagent automatic biochemical analyzer, such as Beckman DXC800, Hitachi 7600 and other automatic analyzers, and uses the endpoint method for determination. Place R1 and R2 on the set positions, and place distilled water, standard products and samples to be tested in the corresponding positions in the sample tray, the operation is as follows:

[0027] Table 1 Operating procedure

[0028]

[0029] Calculation of results: The content of AGEs can be obtained according to the calibration curve with the measuring tube ΔA.

Embodiment 2

[0031] Correlation test: Using the reagent in Example 1, compared with the fluorescence spectrometry, 40 cases of clinical serum samples were detected at the same time, the test results are shown in Table 2, and the results prove that the two have a good correlation.

[0032] Table 2 Example 1 reagent and fluorescence spectrometry method for detecting AGEs content comparison detection results (ng / mL)

[0033]

Embodiment 3

[0035] This example only changes the particle size and the proportion of the latex microspheres, and selects the same sample to detect. The linear relationship between Example 3 and Example 1 is shown in Table 3. The standard concentration is 30ng / mL, and the results show that the implementation The linearity of example 1 reagent is better than embodiment 3.

[0036] Table 3 Linear relationship results

[0037] serial number Example 1 Reagent Example 3 Reagent 0 0 0 1 3.6 3.74 2 10.2 7.51 3 18.9 15.2 4 29.3 29.3 correlation coefficient 0.996 0.929

[0038] Analytical Sensitivity Verification Test:

[0039] Use Example 1, Example 3 and fluorescence spectroscopy (comparison method) to detect samples with known concentrations, and record the difference in absorbance. The test results are shown in Table 4.

[0040] Table 4 Analytical Sensitivity Test Results

[0041]

[0042] Based on the above results, it can be seen t...

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Abstract

The invention relates to a reagent for determining AGEs in human serum by latex immunoturbidimetry; the reagent specifically provided by the invention includes a reagent R1 and a reagent R2, wherein the reagent R1 contains a carbonate buffer solution, sodium chloride, sucrose, bovine serum albumin, and ProClin300; the reagent R2 contains a carbonate buffer solution, sodium chloride, sucrose, bovine serum albumin, anti human AGEs antibody latex microspheres, polyethylene glycol 6000, potassium laurate, gelatin, glycerin and ProClin300. The reagent has the advantages of good stability and linearity, high specificity, high analysis sensitivity, simple operation, easy storage and the like, and can be used for common fully automatic biochemical analyzers in clinic.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to an AGEs detection reagent and a detection method using the detection reagent. Background technique [0002] AGEs are stable, irreversible compounds produced by non-enzyme-catalyzed reactions between amino groups and reducing sugars in proteins, fats, and nucleic acids. At the same time, they can combine with human tissues and cells and cause serious damage. Its source in the organism, on the one hand, a large amount of sugar will react with protein, on the other hand, this substance exists in the food eaten and is taken into the body. Because its accumulation will cause various complications such as diabetes, such as diabetic nephropathy, Alzheimer's disease, atherosclerosis, cataract, etc., and affect various systems of the body, so it is a quick, simple and accurate test in clinical practice. important in diagnosis. At present, its detection methods include radiore...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/546
CPCG01N33/54313
Inventor 王贤俊何丹
Owner 王贤俊
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