Device and method for detection and prevention of crop fungal diseases based on microfluidic chip

Abstract

The invention discloses a preventive detection apparatus and method for fungal diseases of crops based on a micro-fluidic chip, belonging to the field of microbiological detection. According to the preventive detection apparatus, the output shaft of a stepping motor is upwardly arranged and fixedly connected with the center of the micro-fluidic chip; multi-stages of identical micro-fluidic detection channels are arranged on the micro-fluidic chip; a pneumatic solenoid valve group is composed of a plurality of pneumatic solenoid valves with a same structure; the end, where an air inlet is located, of each pneumatic solenoid valve is connected with a chip exhausting opening via a flexible exhausting pipe ; and the other end of the airway of each pneumatic solenoid valve is connected with a minisized air pump via an air outlet of the pneumatic solenoid valve. The preventive detection apparatus employs a rotary micro-fluidic chip capturing structure, has the functions of automatic filtering, negative-pressure suction, enrichment detection, electric air exhausting, etc. and can carry out both on-site high-flux detection of the fungal diseases of a crop in a field and portable, rapid and high-efficiency capturing of fungi on different crops, thereby realizing early detection and early warning of diseases.

Description

technical field [0001] The invention belongs to the field of microorganism detection, in particular to a detection device for one or more crop fungal spores based on an array type rotating microfluidic chip. Background technique [0002] Microbiological detection plays a decisive role in clinical diagnosis, food testing, industrial sewage treatment, preparation of agricultural nutrient solutions, etc., and microfluidic chips have developed rapidly in the field of microbial preventive detection. [0003] Chinese patent application number CN201410623883.1 discloses a plant disease intelligent diagnosis system, including a plant disease intelligent diagnosis host and a computer. The intelligent diagnosis host is equipped with a spectrum scanning device, and the intelligent diagnosis host is equipped with an importable spectrum scanning device. The detection tank, the intelligent diagnosis host is connected to the computer through the data line, it is powerful, and can realize t...

AI Technical Summary

Problems solved by technology

[0003] Chinese patent application number CN201410623883.1 discloses a plant disease intelligent diagnosis system, including a plant disease intelligent diagnosis host and a computer. The intelligent diagnosis host is equipped with a spectrum scanning device, and the intelligent diagnosis host is equipped with an importable spectrum scanning device. The detection tank, the intelligent diagnosis host is connected to the computer through the data line, it is powerful, and can realize the fast, accurate, stable, real-time and non-destructive diagnosis of various plant diseases, but the diagnosis system is used in plants with less fungal spores. At this time, the enrichment of fungal spores cannot be realized, so that real-time early disease detection cannot be realized, and there is hysteresis, and the diagnostic system has many problems such as large detection volume, high cost, inconvenient portability, and requires specialized personnel for detection, which is not conducive to promotion.

Images