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Preparation method and application of sika deer embryo factors

A sika deer and embryonic technology, which is applied in medical formulas, medical preparations containing active ingredients, anti-toxins, etc., can solve the problems of immune rejection, bacterial contamination, systemic infection, etc., and achieve obvious sexual function, prolong life, and curative effect high price effect

Inactive Publication Date: 2017-04-19
沈会强 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

); In 1998, American scientists successfully cultivated human embryonic stem cells in vitro. Since then, scholars at home and abroad have used different stem cells to treat different diseases. Embryonic stem cells, because they are heterogeneous (sheep) and heterogeneous, there are serious complications such as immune rejection, allergies, and systemic infection caused by contaminated bacteria during in vitro culture.
In order to achieve the purpose of repairing tissue cells without serious complications, research departments at home and abroad have done a lot of work, but with little success

Method used

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  • Preparation method and application of sika deer embryo factors
  • Preparation method and application of sika deer embryo factors
  • Preparation method and application of sika deer embryo factors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The present embodiment provides a kind of preparation method of sika deer embryonic element

[0037] 1. Obtain sika deer embryonic cells or embryonic tissues

[0038] 1.1 Source of universal embryonic embryonic cells or embryonic tissues

[0039] 1.1.1 Collection of fertilized eggs:

[0040] The preferred age is 1.5-3 years old, healthy female sika deer with infectious diseases → injection of estrogen intake → ovulation → fertilization → extraction, and embryonic stem cells are taken from fertilized eggs that have developed for 6-18 days. The present invention selects the sika deer cultivated by Guangdong Institute of Agricultural Sciences.

[0041] 1.1.2 In vitro culture:

[0042] The pluripotent stem cells were obtained by in vitro culture of sika deer fertilized eggs.

[0043] 1.1.3 Artificial deer embryo abortion:

[0044] The gestation period of deer is about 230 days, giving birth in about 7 and a half months, and deer embryos are obtained within 40 days afte...

Embodiment 2

[0100] SDEF Therapeutic Animal Experiments

[0101] Choose large and small white mice, rabbits, dogs, and monkeys to make models of different wounds and 15 different diseases, and apply control and SDEF intervention to treat more than 1,000 different animal experiments successively. The models include 15 representative diseases of the following systems: (1) Canine craniocerebral injury model; (2) monkey cerebral ischemia model; (3) mouse tumor model; (4) canine chronic obstructive pulmonary disease model; (5) canine gastric and duodenal ulcer model; (6) Canine liver cirrhosis (cirrhosis) model; (7) Canine hypertension model; (8) Canine acute myocardial infarction model; (9) Canine diabetes model; (10) Canine limb fracture model; (11) Canine aplastic disorder (12) Canine Renal Failure Model; (13) Canine Condyloma Acuminata Model; (14) Rabbit Arteriosclerotic Obliterans Model; (15) Anti-aging Model of Rats. The therapeutic effects of 15 representative diseases in each system ar...

Embodiment 3

[0107] Clinical application trials of SDEF

[0108] 1. Diseases treated by SDEF:

[0109] Embryotherapy is suitable for diseases of various systems, including sub-health, promoting normal growth and development of children, immune system deficiency diseases, infectious diseases, tumors (various benign and malignant tumors), nervous system diseases, respiratory system diseases, digestive system diseases, Cardiovascular system diseases, endocrine system diseases, skeletal and muscular system diseases, blood system diseases, urinary system diseases, reproductive system diseases, sexual function diseases, peripheral vascular diseases, skin diseases, difficult diseases of eyes, ears, nose, pharynx and larynx , anti-aging and beauty, etc.

[0110] 2. Number of disease cases treated by SDEF: More than 1,000 cases of sick sika deer have been treated with various diseases and anti-aging treatment methods.

[0111] 3. Treatment options for SDEF:

[0112] ①Selection of SDEF: According...

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Abstract

The invention discloses a preparation method of sika deer embryo factors. The method comprises the following processes of performing cell membrane breaking treatment on sika deer embryonic stem cells, and removing impurities to obtain a mixture of sika deer universal embryo factors and sika deer directional embryo factors. The invention furthermore discloses the sika deer embryo factors obtained by adopting the preparation method, and an application of the sika deer embryo factors. According to the sika deer embryo factors, the preparation method and the application, the mixture of the sika deer universal embryo factors and the sika deer directional embryo factors is obtained by separation from the sika deer embryonic stem cells, so that various cells in a human body can be kept viable and the lives of working cells can be prolonged; injured cells are repaired to recover cell functions; in-vivo resting cells are activated and effective working cells are supplemented; the attenuation of the stem cells is protected and the stem cell generation is facilitated; and the resting stem cells are activated and differentiated into new cells for replacing dead cells to achieve the effect of recovering the visceral organs, the treatment effect of existing stem cell transplantation is exceeded, and no complications, such as immunological rejection, anaphylaxis, infection and the like, possibly occurring after the stem cell transplantation are generated.

Description

technical field [0001] The invention relates to the technical field of pharmaceuticals, in particular to a sika deer embryogen, and specifically relates to the preparation method and application of the sika deer embryoin. Background technique [0002] Although there are thousands of human diseases, there is only one real disease, which is cell disease. If there is a problem with the cell metabolism of any organ, and the cell death reaches a certain level, then this organ will get sick, which is the main cause of cell failure. There are two aspects: one is the lack of cell nutrition and oxygen supply, and the other is that the metabolic waste cannot be discharged in time, which leads to cell poisoning. Now the understanding of the disease is only the manifestation of the disease, not the real cause, and the treatment is mostly symptomatic. (such as the treatment of pain, fever, etc.) or allopathic treatment (removal of tumors, radiotherapy, chemotherapy, etc.), but cannot sta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/545A61P9/00A61P9/10A61P3/10A61P35/00A61P1/04A61P1/16A61P39/06
CPCA61K35/545
Inventor 沈会强谢培增刘江郑晓云谢成沈明阳
Owner 沈会强
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