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Brevibacillus brevis strain and application thereof

A technology of Bacillus brevis and strains, applied in the field of microorganisms, can solve problems such as unsafe, chemical prevention and control of polluted environment, Bacillus brevis strains, etc., and achieve huge application potential, high control effect and good control effect

Active Publication Date: 2017-03-08
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of Bacillus brevis strain and its application in order to solve the pollution environment and unsafe problems existing in chemical control

Method used

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  • Brevibacillus brevis strain and application thereof
  • Brevibacillus brevis strain and application thereof
  • Brevibacillus brevis strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Isolation, purification and identification of Bacillus brevis:

[0024] Take soil samples evenly from the tomato planting fields in Shunyi, mix 10g of soil with 100mL of sterilized water to make a suspension, and then carry out gradient dilution in turn, take 10000 times diluted soil mixture 100μL and spread it on the LB medium plate, The formula of LB medium is: yeast powder 5g / L, peptone 10g / L, NaCl 10g / L, agar powder 1.3-1.5g / 100mL, dilute to 1L and seal. After sterilizing at 121° C. for 20 minutes at high temperature and high pressure, spread it into a flat plate in a petri dish. The medium was cultured at 37°C. After the tissue pieces grew colonies, a single colony was picked and purified on LB medium for classification and identification. The strains were morphologically determined according to the Bergey's Manual of Bacterial Identification.

[0025] Pick a single colony with a sterilized toothpick, put it into an EP tube filled with 300 μL of bacterial lysate ...

Embodiment 2

[0030] 1, the preparation method of Bb-1 bacterial strain fermented liquid, comprises the following steps:

[0031] (1) Streak-inoculate Bacillus brevus Bb-1 on LB medium, culture it on a plate in a constant temperature incubator for 60 hours, set the culture temperature at 37°C, and obtain a single colony;

[0032] (2) Inoculate the single colony of Bacillus brevus Bb-1 in the seed culture medium (LB medium), shake and cultivate in a constant temperature shaking table, the rotating speed is 160rpm, the temperature is 37°C, and shake and cultivate for 36h; liquid seed culture medium The formula is LB medium, that is, yeast powder 5g / L, peptone 10g / L, NaCl 10g / L, and the volume is adjusted to 1L;

[0033] (3) Inoculate the seed liquid into the sterilized fermentation medium for fermentation culture, the inoculum size is 1%, the temperature is set at 37°C, and the obtained concentration is 10 6 cfu / mL fermentation broth (stock solution); liquid fermentation medium formula is LB...

Embodiment 3

[0045] 1, the preparation method of Bb-1 bacterial strain fermented liquid, comprises the following steps:

[0046] (1) Streak-inoculate Bacillus brevus Bb-1 on LB medium, and culture it on a plate in a constant temperature incubator for 60 hours at a temperature of 37°C to obtain a single colony;

[0047] (2) Inoculate a single colony of Bacillus brevus Bb-1 in the seed medium, and cultivate it in a constant temperature shaker with a rotation speed of 160rpm and a temperature of 37°C for 36 hours; the formula of the liquid seed medium is LB medium , that is, yeast powder 5g / L, peptone 10g / L, NaCl 10g / L, dilute to 1L;

[0048] (3) Inoculate the seed liquid into the sterilized fermentation medium for fermentation culture, the inoculum size is 1%, the temperature is set at 37°C, and the obtained concentration is 10 6 cfu / mL fermentation broth; liquid fermentation medium formula is LB medium, that is, yeast powder 5g / L, peptone 10g / L, NaCl 10g / L, and the volume is adjusted to 1L...

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Abstract

The invention discloses a brevibacillus brevis strain and application thereof. The strain is brevibacillus Bb-1, and is preserved in CGMCC (China General Microbiological Culture Collection Center); the preservation number is CGMCC NO.12365. The invention also provides application of an antibiological inoculants prepared from the brevibacillus brevis Bb-1 to preparation and treatment of root-knot nematode. The brevibacillus brevis is separated from soil, has a good prevention effect on the root-knot nematode, can easily realize the fixed planting in the soil, and is favorable for the early stage prevention and control of the root-knot nematode harm. The strain has huge application potential on the problem solving of the prevention and the treatment of the root-knot nematode, and is favorable for realizing the high-yield safe and harmless production of vegetables; the killing rate of the fermentation liquid of the brevibacillus brevis Bb-1 strain on the root-knot nematode reaches 100 percent; the prevention and treatment effect in the potted plant experiments reaches 81.6 percent; the prevention and treatment effect is high and stable; the agricultural production requirements are met.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Bacillus brevis and an application thereof. Background technique [0002] Root-knot nematode (Meloidogyne) is a highly specialized omnivorous plant pathogenic nematode. Nematodes known to harm vegetables mainly include root-knot nematode peanut, root-knot nematode northern, root-knot nematode southern, root-knot nematode java, and root-knot nematode beet. Nematodes have a wide range of hosts, often harming more than 30 kinds of vegetables such as melons, solanaceous fruits, beans, radishes, carrots, lettuce, and cabbage, and can also spread some fungal and bacterial diseases. Root-knot nematode is an important crop pathogen, causing losses of about billions of dollars every year. Root-knot nematodes mainly infect the roots of crops. After infection, the roots swell to form huge root knots, which can be connected to form beads. Because the roots ar...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P5/00C12R1/01
CPCA01N63/10C12N1/20C12N1/205C12R2001/01
Inventor 谢丙炎茆振川杨宇红凌键张晓平
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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