Kit and detection method thereof for SNP detection of aspirin individualized medication related genes

Abstract

The invention discloses a kit and a detection method thereof for SNP detection of aspirin individualized medication related genes. The kit consists of two forward primers and a reverse primer for detecting rs10306114 site of a PTGS1 gene, two forward primers and a reverse primer for detecting rs1057910 site of a CYP2C9 gene, two forward primers and a reverse primer for detecting rs2836051 site of a CYP2D6 gene, and two forward primers and a reverse primer for detecting rs12248560 site of a CYP2C19 gene. With the application of the kit provided by the invention, high-throughput detection can be conducted on the four sites and a genotyping results can be intuitively distinguished through software, so that the purposes of conducting quantitative control on aspirin measurement and reducing side effects to the greatest extent are achieved.

Description

technical field [0001] The invention relates to a kit for multiplex gene detection and a detection method thereof, in particular to a kit for detection of SNP of a gene related to individualized medicine of aspirin and a detection method thereof. Background technique [0002] Aspirin is the basic drug for the treatment of acute coronary syndrome and antithrombotic after percutaneous coronary intervention, and is widely used in the primary and secondary prevention of cardiovascular and cerebrovascular diseases. It is clinically found that some patients cannot effectively inhibit platelet activity despite long-term low-dose aspirin, that is, aspirin resistance. [0003] PTGS1 encodes COX-1, and PTGS1 gene polymorphisms can cause base substitutions and changes in promoter junctions, significantly affect the function of introns or exons, change the conformation of COX-1 protein, and make aspirin effective against COX-1. The sensitivity of aspirin is not uniform, thereby affecti...

AI Technical Summary

Problems solved by technology

Sequencing technology is recognized as the gold standard for detecting gene mutations, but it is difficult to form due to high equipment costs, long detection cycle, low detection throughput, low detection sensitivity, high technical requirements for experimenters, and cumbersome results determination steps. Commercial diagnostic products; the detection sensitivity of restriction fragment length polymorphism analysis is also not high, and the operation steps are cumbersome. The detection results still need to be verified again by the next generation sequencing method, especially when the sample size is large, it is easy to cause crossover of PCR products Contamination and prone to insufficient or excessive enzyme digestion and false negative or false positive results, so it cannot be applied clinically

Chip detection is not suitable for the development of clinical detection kits due to its poor accuracy and repeatability of detection results and long experimental cycle.

The detection method of fluorescent quantitative PCR has the advantages of low cost, high sensitivity, strong specificity, and good repeatability of results. It is a very good detection method for detecting SNPs, but the ordering cost of conventional Taqman probe method probes is too high , it is difficult to ensure that all primers and probes can achieve very good amplification results under the same amplification conditions when multiple sites are detected at the same time

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