Preparation method of biological mimetic tissue adhesive

Abstract

The invention relates to a preparation method of a biological mimetic and structural bionic tissue adhesive in order to overcome disadvantages of present technological technologies for preparing mussel mucoprotein bionic biological tissue adhesives and improve the viscosity, the biocompatibility and the structural bionic property of a material. The preparation method comprises the following steps: grafting a natural polymer material with dopamine or other catechol group-containing derivatives through an amidation reaction by adopting DMTMM as a carboxyl group activator; and further modifying the grafted natural polymer material with lysine or polylysine by adopting the same technology to obtain the novel biological mimetic and structural bionic tissue adhesive containing dopamine analog and polylysine analog. The tissue adhesive plays a full role in tissue adhesion in wet and physiologic environment, has good tissue adherence and high biological safety, and can be slowly degraded in the tissue healing process until complete degradation. The preparation method of the biological adhesive has the advantages of simplicity, mild reaction conditions, increase of the reaction efficiency, improvement of the operability of the enterprise production process, and facilitation of amplified production of enterprises.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a biomimetic adhesive and a preparation method thereof. It has potential application value in the bonding of traumatic skin tears and surgery-free suturing. Background technique [0002] Over the years, due to the advantages of strong sutures and difficult wound dehiscence, suturing wounds with sutures, rivets and other materials or instruments is the most commonly used and most effective wound bonding method in clinical practice. However, with the rapid development of modern medicine, the clinical requirements for surgical methods and auxiliary materials are getting higher and higher. In order to reduce the complexity of surgery and trauma to patients, medical adhesives have been developed rapidly and widely used. . [0003] Medical adhesives refer to preparations, materials or substances that can be adhered to surfaces or cause adhesion between surfaces in medica...

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Problems solved by technology

[0007] At present, the research based on marine biological mussels mainly includes two approaches. One is to simply use dopamine to form polydopamine by controlling the conditions for surface coating of metal materials and inorganic materials. The inertness of the modified material surface can be overcome at the same time. The biocompatibility and cell adhesion performance of the material are improved, and the biological activity of the material is improved. The second is to graft dopamine and its derivatives to other polymer materials through chemical grafting to endow similar bioadhesiveness. Domestic The patent "Bioadhesive and its preparation method" (Application No. 201410852796.3) provides a method for preparing bioadhesive through 3-step modification. In this paper, the traditional carboxyl activator carbodiimide (EDC) is used and N-hydroxysuccinimide (NHS), although the adhesive properties of bioadhesives can be improved, aldehyde groups, organic solvents, etc. will be introduced in the reaction process, and the activation mode of EDC combined with NHS has a great influence on the reaction system. The pH value is highly dependent, resulting in many problems such as cumbersome preparation process and poor biocompatibility

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