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Strain screening method with efficient desulfurization and denitrification activities simultaneously

A strain screening and denitrification technology, applied in the biological field, can solve the problems of increasing equipment and raw material input, increasing operating costs, etc., achieve the effects of lasting sulfide degradation activity and ammonia nitrogen degradation activity, reducing operating costs, and avoiding load

Active Publication Date: 2017-01-11
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, it increases the operating cost, on the other hand, it increases the input of equipment and raw materials, and synchronous desulfurization and denitrification has become a new research direction. There is no research on the use of biological treatment for synchronous denitrification and desulfurization, and control the desulfurization products in the elemental sulfur stage. related reports

Method used

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  • Strain screening method with efficient desulfurization and denitrification activities simultaneously
  • Strain screening method with efficient desulfurization and denitrification activities simultaneously
  • Strain screening method with efficient desulfurization and denitrification activities simultaneously

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Experimental program
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Effect test

Embodiment 1

[0030] The first screening procedure is as follows:

[0031] Take 20g of biochemical sludge from the ash yard of the power plant and put it into a petri dish filled with the primary screening medium, put the petri dish into a constant temperature shaker at 30°C for cultivation, keep the speed of the constant temperature shaker at 185 rpm, and the culture time is 5 days;

[0032] The primary screening medium consisted of 15g Na 2 S 2 o 3 , 9g KNO 3 , 6g (NH 4 )2HPO 4 , 1.5g MgCl 2 , 6g NaHCO 3 , 1.5g of cysteine, 30ml of vitamin nutrient solution and 30ml of trace element nutrient solution were mixed, adjusted to pH 7.0, and obtained after sterilizing at 121°C for 20 minutes;

[0033] The program of second step purification separation is:

[0034] Inoculate the strains in the petri dish after the first shaker culture to the solid medium, and place the solid medium inoculated with the strains in a biochemical incubator at 30°C for 3 days;

[0035] The solid medium cons...

Embodiment 2

[0045] The first screening procedure is as follows:

[0046] Take 20g of biochemical activated sludge from Shaying Sewage Treatment Plant and put it into a petri dish filled with primary screening medium, put the petri dish into a constant temperature shaker at 30°C for cultivation, keep the speed of the constant temperature shaker at 185 rpm, and cultivate The time is 5 days;

[0047] The primary screening medium consisted of 15g Na 2 S 2 o 3 , 9g KNO 3 , 6g (NH 4 ) 2 HPO 4 , 1.5g MgCl 2 , 6g NaHCO 3 , 1.5g of cysteine, 30ml of vitamin nutrient solution and 30ml of trace element nutrient solution were mixed, adjusted to pH 7.0, and obtained after sterilizing at 121°C for 20 minutes;

[0048] The program of second step purification separation is:

[0049] Inoculate the strains in the petri dish after the first shaker culture to the solid medium, and place the solid medium inoculated with the strains in a biochemical incubator at 30°C for 3 days;

[0050] The solid m...

Embodiment 3

[0060] The first screening procedure is as follows:

[0061] Take 30g of activated sludge from the aeration tank of Zhuangxi Sewage Treatment Plant and put it into a petri dish filled with primary screening medium, put the petri dish into a constant temperature shaker at 30°C for cultivation, and keep the speed of the constant temperature shaker at 185 rpm min, the culture time is 5 days;

[0062] The primary screening medium consisted of 15g Na 2 S 2 o 3 , 9g KNO 3 , 6g (NH 4 ) 2 HPO 4 , 1.5g MgCl 2 , 6g NaHCO 3 , 1.5g of cysteine, 30ml of vitamin nutrient solution and 30ml of trace element nutrient solution were mixed, adjusted to pH 7.0, and obtained after sterilizing at 121°C for 20 minutes;

[0063] The program of second step purification separation is:

[0064] Inoculate the strains in the petri dish after the first shaker culture to the solid medium, and place the solid medium inoculated with the strains in a biochemical incubator at 30°C for 3 days;

[0065]...

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Abstract

The invention provides a strain screening method with efficient desulfurization and denitrification activities simultaneously and belongs to the technical field of biology. The method comprises the following steps: primary screening in the first step, purification and separation in the second step, biochemical culture in the third step and multiplication culture in the fourth step. According to the screening method provided by the invention, by setting screening and culturing conditions, strains with relatively high desulfurization and denitrification activities can be obtained. For strains obtained by adopting the screening method, the sulfide degradation efficiency is higher than 99%, and the ammonia nitrogen degradation efficiency is higher than 70%. The strain can save water treatment facility investment, ensure the sewage treatment effect, reduce investment for chemical treatment and energy consumption, and lower the sewage treatment cost, which is at least lowered by 0.20 yuan / cubic meter, has no secondary pollution, and can finally eliminate the influence to surrounding water environment and atmospheric environment; furthermore, the strain can be applied to treatment of petrochemical wastewater and domestic sewage, and has good environment and social benefits.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain screening method with simultaneous high-efficiency desulfurization and denitrification activities. Background technique [0002] At present, the main objects of oilfield produced water treatment and reuse are petroleum, ammonia nitrogen, and sulfide: sulfide is one of the main factors that cause serious corrosion in oilfield water injection systems and industrial circulating cooling water; ammonia nitrogen is one of the substances that cause eutrophication of water bodies one. [0003] Refinery stripping wastewater contains a lot of H 2 S and NH 3 , directly discharged into the purified water workshop without treatment, will inevitably cause an impact on the conventional biochemical system, resulting in unqualified discharge of the total effluent; the volatile gas in the water tank of the acidic water stripping device has a serious stench, and aggravates the corrosion of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02
CPCC12N1/02
Inventor 王晓慧郭爱洪陈健飞吕德东刘璐席琦
Owner CHINA PETROLEUM & CHEM CORP
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