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Trichoderma harzianum strain Th-N5 resisting carbendazim and application of trichoderma harzianum strain Th-N5

A technology of carbendazim and bacterial strains, applied in the direction of application, fungicides, fungi, etc., can solve the problems of reducing the amount of chemical pesticide use and residues, and achieve the effects of reducing the amount of use, strong drug resistance, and solving the problem of pesticide residues

Active Publication Date: 2017-01-04
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by this invention is to overcome the defects and deficiencies of the existing mulberry ginkgo disease prevention and treatment technology, to provide a carbendazim-resistant Trichoderma harzianum strain Th-N5 and its application, and the Trichoderma harzianum strain Th-N5 is effective for the prevention and treatment of Mulberry ginkgo disease has a significant effect, and the Trichoderma harzianum strain Th-N5 has strong resistance to carbendazim, mixed with low doses of carbendazim, can completely control mulberry ginkgo disease, greatly reducing the use of chemical pesticides The amount of use can solve the problem of pesticide residues in mulberries without polluting the environment and the ecological environment of farmland.

Method used

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  • Trichoderma harzianum strain Th-N5 resisting carbendazim and application of trichoderma harzianum strain Th-N5
  • Trichoderma harzianum strain Th-N5 resisting carbendazim and application of trichoderma harzianum strain Th-N5
  • Trichoderma harzianum strain Th-N5 resisting carbendazim and application of trichoderma harzianum strain Th-N5

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Experimental program
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Effect test

Embodiment 1

[0033] Isolation and identification of embodiment 1 Trichoderma harzianum Th-N5 bacterial strain

[0034] 1. Isolation of strains

[0035] (1) Materials: The target strain was isolated from mulberry ginkgo that was naturally infected in mulberry orchards in Guangzhou.

[0036] (2) Experimental conditions

[0037] Potato dextrose agar medium (PDA): Pour 200g potato + 20g glucose + 17-20g agar powder into a beaker and add water to make the total volume 1000ml. Sterilized by autoclaving (121°C, 30min).

[0038] Aseptic operating conditions: All utensils and utensils must be sterilized by autoclaving (121°C, 30min), and inoculation and other operations are carried out in an ultra-clean workbench.

[0039] Culture conditions: Culture in an incubator at 26±1°C under light (14L:10D). After colonies are formed, transfer to a PDA slope, and then transfer to a 4°C refrigerator for storage.

[0040] (3) Isolation of strains

[0041] Use 5% sodium hypochlorite solution to sterilize t...

Embodiment 2

[0081] The pathogenicity determination of the crude toxin of embodiment 2 Trichoderma harzianum Th-N5 to Sclerotinia sclerotiorum

[0082] 1. Bioassay is one of the effective means to detect the lethality and lethality rate of biocontrol fungi to target pathogenic bacteria, and can provide an important reference for comprehensive evaluation of the biocontrol potential of the fungus. In the present invention, the pathogenicity of the crude toxin of Trichoderma harzianum Th-N5 to Sclerotinia sclerotiorum is determined, so as to screen the optimal concentration used in the control.

[0083] Tested strains: Trichoderma harzianum Th-N5 strain, Sclerotinia sclerotiorum.

[0084] (1) Preparation of crude toxin of test strains and Sclerotinia mycelium block

[0085] The spores of the purified Trichoderma harzianum Th-N5 strain were prepared with 0.05% Tween-80 sterile water to a concentration of 1×10 8Conidia / mL spore suspension, then take 2ml and add it to 200ml Czapek culture medi...

Embodiment 3

[0094] Example 3 Trichoderma harzianum Th-N5 strain mixed with low concentration carbendazim to control mulberry ginkgo

[0095] 1. Method

[0096] Tested strains and carbendazim: Trichoderma harzianum Th-N5 strain, carbendazim was purchased from the Institute of Plant Protection, Guangdong Academy of Agricultural Sciences.

[0097] (1) Preparation of the spore suspension of the tested strain and the mixture of carbendazim

[0098] The purified Trichoderma harzianum Th-N5 strain was cultured on a PDA plate in an incubator (14L:10D) at 25±1°C for 5 days, and the spores were collected by adding 0.3% Tween-80 sterile water. Stir the spore suspension on a magnetic stirrer for 20 minutes. After the spores are evenly dispersed, use medical gauze to filter out impurities to obtain a spore suspension. Use a hemocytometer to determine the concentration of the spores and make 1×10 7 The spore liquid of spore / ml is ready for use. Add carbendazim to make 1×10 7 The spore / ml spore liqu...

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Abstract

The invention discloses a trichoderma harzianum strain Th-N5 resisting carbendazim and application of the trichoderma harzianum strain Th-N5. The trichoderma harzianum strain Th-N5 is preserved at China Center for Type Culture Collection on May 6, 2016, and the preservation number is CCTCC NO. M2016251. By virtue of the study of infection biology and indoor bioassay, the strain has good effect for preventing Sclerote disease of mulberry fruit; and the strain has relatively high resistance to the chemical pesticide Carbendazim. The strain is cooperated with a low dosage of carbendazim, the Sclerote disease of mulberry fruit can be thoroughly controlled, the use amount of the chemical pesticide is greatly reduced, and the pesticide residue problem in the mulberry fruits can be solved. The strain is a local strain in China and is not introduced from foreign countries, thereby being adaptive to the local natural environment. In addition, the trichoderma harzinum is a biocontrol fungus, and is used as a living body biological pesticide, the tridoderma harzinum has a brand new acting mechanism different from the existing chemical insecticide, is pollution-free to the environment, has no residue and adapts to the production requirement of the organic food.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to a carbendazim-resistant Trichoderma harzianum strain Th-N5 and its application in mixing with low-dose carbendazim to control mulberry ginkgo disease. Background technique [0002] Mulberry ginkgo disease is a common name for sclerotinia, also known as mulberry ginkgo disease, which belongs to fungal diseases and is the main disease on mulberries. Sclerotias clerotiorum ), C. mulberry ( Ciboria shiraiana ), Cupella carunculus ( Ciboria carunculoides ) and G. nucleatum ( Scleromitulashiraiana ). Mulberry ginkgo disease is highly contagious and spreads quickly. If the disease is mild, it will affect the yield and quality of mulberry, and if it is serious, it will cause the mulberry to fail to mature and harvest normally, resulting in a large-scale reduction in production and no harvest of mulberry. It will cause devastating disasters to the fruit and mulberry ind...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01N47/18A01P3/00C12R1/885
CPCA01N47/18A01N63/30C12N1/145C12R2001/885A01N2300/00
Inventor 黄振
Owner SOUTH CHINA AGRI UNIV
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