Cholesterol content detection method

A detection method and technology for cholesterol, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of long detection time, difficult to guarantee the accuracy of detection results, poor separation effect, etc., to simplify the sample processing process, eliminate the interference of impurities, The effect of improving accuracy

Active Publication Date: 2016-12-07
BY HEALTH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The other two methods require special equipment, and the detection time is longer. At the same time, due to the large amount of impurities in the sample, the separation effect is poor, and the accuracy of the detection results is difficult to guarantee.

Method used

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  • Cholesterol content detection method
  • Cholesterol content detection method
  • Cholesterol content detection method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The detection of embodiment 1 cholesterol content

[0053] (1) Detection method

[0054] Instrument: Agilent GC / MS.

[0055] Reagent reagent:

[0056] Isooctane (AR);

[0057] Source of Cholesterol Reference Substance: DR Lot Number: 00219 Purity: 95.0%.

[0058] Analysis steps:

[0059] (1) Instrument parameters

[0060] Injection port: 250°C; Injection volume: 1 μL; Split ratio: 20:1.

[0061] Chromatographic column: flow rate 1.5mL / min; HP-5ms 30m*0.25mm*0.25μm.

[0062] Column oven: column temperature 275°C, keep for 13min.

[0063] Carrier gas: Helium (99.999%).

[0064] Interface temperature: 280°C.

[0065] Ionization mode: EI Ionization voltage: 70eV.

[0066] Selected ions: 386 (quantitative), 275, 105.

[0067] Electron Multiplier Voltage: Autotune value increased by 200V.

[0068] Detector: MSD.

[0069] (2) Preparation of control stock solution:

[0070] Accurately weigh 20 mg of the cholesterol reference substance, place it in a 10 mL brown volu...

Embodiment 2

[0137] The detection of embodiment 2 cholesterol content

[0138] Analysis steps:

[0139] (1) Instrument parameters

[0140] Injection port: 240°C; Injection volume: 1 μL; Split ratio: 20:1.

[0141]Chromatographic column: flow rate 1.8mL / min; HP-5ms 30m*0.25mm*0.25μm.

[0142] Column oven: column temperature 260°C, keep for 13min.

[0143] Carrier gas: Helium (99.999%).

[0144] Interface temperature: 290°C.

[0145] Ionization mode: EI Ionization voltage: 70eV.

[0146] Selected ions: 386 (quantitative), 275, 105.

[0147] Electron Multiplier Voltage: Autotune value increased by 150V.

[0148] Detector: MSD.

[0149] (2) Preparation of control stock solution:

[0150] Accurately weigh 20mg of the cholesterol reference substance, put it in a 10mL brown volumetric flask, add ether to dissolve and adjust the volume to the mark, shake well, and you get it.

[0151] (3) Standard working curve drawing:

[0152] Precisely pipette 200 μL, 500 μL, 1000 μL, 1500 μL, and 20...

Embodiment 3

[0165] The detection of embodiment 3 cholesterol content

[0166] Analysis steps:

[0167] (1) Instrument parameters

[0168] Injection port: 260°C; Injection volume: 1 μL; Split ratio: 20:1.

[0169] Chromatographic column: flow rate 2.0mL / min; HP-5ms 30m*0.25mm*0.25μm.

[0170] Column oven: column temperature 280°C, keep for 13min.

[0171] Carrier gas: Helium (99.999%).

[0172] Interface temperature: 300°C.

[0173] Ionization mode: EI Ionization voltage: 70eV.

[0174] Selected ions: 386 (quantitative), 275, 105.

[0175] Electron Multiplier Voltage: Autotune value increased by 180V.

[0176] Detector: MSD.

[0177] (2) Preparation of control stock solution:

[0178] Accurately weigh 20mg of the cholesterol reference substance, put it in a 10mL brown volumetric flask, add n-hexane to dissolve it, adjust the volume to the mark, shake well, and you get it.

[0179] (3) Standard working curve drawing:

[0180] Precisely pipette 200 μL, 500 μL, 1000 μL, 1500 μL, an...

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Abstract

The invention relates to the field of quality control technology, especially to a cholesterol content detection method. The detection method comprises the following steps: a sample to be measured is dissolved in an organic solvent to obtain a test solution, and content of cholesterol in the test solution is detected by a gas chromatography-mass spectrum technology, wherein the organic solvent is one selected from isooctane, ether and hexane or a mixture of more than two selected from isooctane, ether and hexane. By the detection method, detection time can be greatly shortened, and detection accuracy can be raised. through the cholesterol content detection method, linearity, precision, repeatability, stability, blank, detection limit, quantification limit and recovery rate tests all meet requirements of GB / T27404-2008 ''laboratory quality control specification''. It proves that the method is scientific and effective and can be adopted for achieving the purpose of quality control of cholesterol content in animal and plant samples.

Description

technical field [0001] The invention relates to the technical field of quality control, in particular to a method for detecting cholesterol content. Background technique [0002] Cholesterol, also known as cholesterol, is a derivative of cyclopentane polyhydrophenanthrene. Cholesterol is widely present in animals, especially in brain and nerve tissue, and is also high in kidney, spleen, skin, liver and bile. Its solubility is similar to that of fat, insoluble in water, and easily soluble in solvents such as ether and chloroform. Cholesterol is an indispensable and important substance in animal tissue cells. It not only participates in the formation of cell membranes, but also is a raw material for the synthesis of bile acids, vitamin D and steroid hormones. Cholesterol can also be converted into bile acids, steroid hormones, and 7-dehydrocholesterol after metabolism, and 7-dehydrocholesterol can be converted into vitamin D3 after ultraviolet irradiation. Cholesterol mainl...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张喜金黎小兰苏昭仑吴振知
Owner BY HEALTH CO LTD
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