Cholesterol content detection method
A detection method and technology for cholesterol, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of long detection time, difficult to guarantee the accuracy of detection results, poor separation effect, etc., to simplify the sample processing process, eliminate the interference of impurities, The effect of improving accuracy
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Embodiment 1
[0052] The detection of embodiment 1 cholesterol content
[0053] (1) Detection method
[0054] Instrument: Agilent GC / MS.
[0056] Isooctane (AR);
[0057] Source of Cholesterol Reference Substance: DR Lot Number: 00219 Purity: 95.0%.
[0058] Analysis steps:
[0059] (1) Instrument parameters
[0060] Injection port: 250°C; Injection volume: 1 μL; Split ratio: 20:1.
[0061] Chromatographic column: flow rate 1.5mL / min; HP-5ms 30m*0.25mm*0.25μm.
[0062] Column oven: column temperature 275°C, keep for 13min.
[0063] Carrier gas: Helium (99.999%).
[0064] Interface temperature: 280°C.
[0065] Ionization mode: EI Ionization voltage: 70eV.
[0066] Selected ions: 386 (quantitative), 275, 105.
[0067] Electron Multiplier Voltage: Autotune value increased by 200V.
[0068] Detector: MSD.
[0069] (2) Preparation of control stock solution:
[0070] Accurately weigh 20 mg of the cholesterol reference substance, place it in a 10 mL brown volu...
Embodiment 2
[0137] The detection of embodiment 2 cholesterol content
[0138] Analysis steps:
[0139] (1) Instrument parameters
[0140] Injection port: 240°C; Injection volume: 1 μL; Split ratio: 20:1.
[0141]Chromatographic column: flow rate 1.8mL / min; HP-5ms 30m*0.25mm*0.25μm.
[0142] Column oven: column temperature 260°C, keep for 13min.
[0143] Carrier gas: Helium (99.999%).
[0144] Interface temperature: 290°C.
[0145] Ionization mode: EI Ionization voltage: 70eV.
[0146] Selected ions: 386 (quantitative), 275, 105.
[0147] Electron Multiplier Voltage: Autotune value increased by 150V.
[0148] Detector: MSD.
[0149] (2) Preparation of control stock solution:
[0150] Accurately weigh 20mg of the cholesterol reference substance, put it in a 10mL brown volumetric flask, add ether to dissolve and adjust the volume to the mark, shake well, and you get it.
[0151] (3) Standard working curve drawing:
[0152] Precisely pipette 200 μL, 500 μL, 1000 μL, 1500 μL, and 20...
Embodiment 3
[0165] The detection of embodiment 3 cholesterol content
[0166] Analysis steps:
[0167] (1) Instrument parameters
[0168] Injection port: 260°C; Injection volume: 1 μL; Split ratio: 20:1.
[0169] Chromatographic column: flow rate 2.0mL / min; HP-5ms 30m*0.25mm*0.25μm.
[0170] Column oven: column temperature 280°C, keep for 13min.
[0171] Carrier gas: Helium (99.999%).
[0172] Interface temperature: 300°C.
[0173] Ionization mode: EI Ionization voltage: 70eV.
[0174] Selected ions: 386 (quantitative), 275, 105.
[0175] Electron Multiplier Voltage: Autotune value increased by 180V.
[0176] Detector: MSD.
[0177] (2) Preparation of control stock solution:
[0178] Accurately weigh 20mg of the cholesterol reference substance, put it in a 10mL brown volumetric flask, add n-hexane to dissolve it, adjust the volume to the mark, shake well, and you get it.
[0179] (3) Standard working curve drawing:
[0180] Precisely pipette 200 μL, 500 μL, 1000 μL, 1500 μL, an...
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