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Bacterial cellulose as well as pollution-free preparation method and applications of bacterial cellulose

A technology of bacterial cellulose and cellulase, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve problems such as high cost and inability to adapt to industrial production, and achieve low pretreatment temperature and excellent biological dissolution. Sex, the effect of shortening the reaction time

Inactive Publication Date: 2016-12-07
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the fermentation medium components of laboratory fermentation to produce bacterial cellulose are mainly expensive reagents, which cannot meet the needs of industrial production

Method used

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  • Bacterial cellulose as well as pollution-free preparation method and applications of bacterial cellulose

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Embodiment 1

[0044] A green preparation method of bacterial cellulose of the present embodiment, the specific preparation steps are as follows:

[0045] (1) Pretreatment of amino acid ionic liquid: uniformly mix crushed 40-mesh bagasse and glycylic amino acid ionic liquid at a ratio of 2% at 80°C, cool after pretreatment for 0.5h, and centrifuge at a speed of 4000rpm 10min obtains precipitate;

[0046] (2) Enzymolysis of the precipitate: Wash the precipitate obtained in step (1) with distilled water to neutrality, then add 6U / mL cellulase to the precipitate, and the mass ratio of the cellulase to the precipitate is 1 / 5. Enzymolysis at 35°C and pH 4.0 for 12 hours, followed by centrifugation at 3000 rpm for 5 minutes to separate undegraded bagasse and enzymolysis;

[0047] (3) Detoxification treatment of enzymolysis solution: first adjust pH to 1.5 with concentrated sulfuric acid, carry out acidification treatment, adjust pH value to 6.0 with calcium oxide after filtration, add 1% gac in ...

Embodiment 2

[0052] A green preparation method of bacterial cellulose of the present embodiment, the specific preparation steps are as follows:

[0053] (1) Pretreatment of amino acid ionic liquid: uniformly mix crushed 40-mesh bagasse and glycylic amino acid ionic liquid at a ratio of 3% at 80°C, cool after pretreatment for 1.0h, and centrifuge at a speed of 4000rpm 10min obtains precipitate;

[0054] (2) Enzymolysis of the precipitate: Wash the precipitate obtained in step (1) with distilled water to neutrality, then add 7U / mL cellulase to the precipitate, and the mass ratio of cellulase to precipitate is 1 / 5. Enzymolysis at 40°C and pH 4.0 for 12 hours, followed by centrifugation at 4000 rpm for 10 minutes to separate undegraded bagasse and enzymolysis solution;

[0055] (3) Detoxification treatment of enzymolysis solution: first adjust the pH to 1.5 with concentrated sulfuric acid, carry out acidification treatment, then adjust the pH value to 6.5 with calcium oxide after filtration,...

Embodiment 3

[0060] A green preparation method of bacterial cellulose of the present embodiment, the specific preparation steps are as follows:

[0061] (1) Pretreatment of amino acid ionic liquid: uniformly mix crushed 50-mesh bagasse and glycylic amino acid ionic liquid at a ratio of 4% at 90°C, cool after pretreatment for 1.5h, and centrifuge at a speed of 5000rpm 20min obtains precipitate;

[0062] (2) Enzymolysis of the precipitate: the precipitate obtained in step (1) was washed with distilled water to neutrality, and then 8U / mL of cellulase was added to the precipitate, and the mass ratio of cellulase to the precipitate was 2 / 5. Enzyme hydrolyze for 18 hours at a temperature of 40°C and pH 4.5, and then centrifuge at 5000 rpm for 10 minutes to separate undegraded bagasse and enzymolyzate;

[0063] (3) Detoxification treatment of enzymolysis solution: first adjust pH to 2.0 with concentrated sulfuric acid, carry out acidification treatment, adjust pH value to 6.5 with calcium oxide...

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Abstract

The invention discloses bacterial cellulose as well as a pollution-free preparation method and applications of the bacterial cellulose, belonging to the technical field of the bacterial cellulose. The method comprises the following steps: carrying out pretreatment by adopting amino acid ionic liquid; carrying out enzymolysis on the precipitate; carrying out detoxification treatment on the enzymatic hydrolysate; preparing a fermentation culture medium; and synthesizing the bacterial cellulose. According to the method, bagasse hydrolysate is taken as a carbon source of the culture medium, so that the cost for synthesizing the bacterial cellulose is reduced. The pretreatment on bagasse adopting the amino acid ionic liquid is a pollution-free and efficient pretreatment means, the amino acid ionic liquid is good in chemical stability and can be recycled, in addition, the amino acid ionic liquid is non-toxic and has excellent biological solubility, the pretreatment temperature is low, the efficiency is high, and the reaction time is shortened. The adopted device for microbial synthesis of the bacterial cellulose is simple and easy to operate, the operation is simple, the raw materials are easily available, and thus the large-scale preparation is facilitated. The prepared bacterial cellulose is high in yield, uniform in grain diameter and excellent in property, and can be taken as a biological material to be applied to the field of medical materials.

Description

technical field [0001] The invention belongs to the technical field of bacterial cellulose, and in particular relates to a bacterial cellulose and its green preparation method and application. Background technique [0002] Bacterial cellulose (Bacterial Cellulose, BC) has been recognized as the cellulose with the best performance and good practical value in the world. As a new type of nanomaterial, it has attracted widespread attention. Bacterial cellulose is a polymer network structure in which glucose molecules are linked by β-1,4-glycosidic bonds. Compared with plant cellulose, bacterial cellulose does not contain lignin, hemicellulose, extracts and other accompanying substances, so it has high purity, high crystallinity and high degree of polymerization; and bacterial cellulose has higher water content, Young's properties such as modulus, fiber ultrafine structure, biodegradability and biocompatibility. These excellent properties of bacterial cellulose make it widely u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/02
CPCC12P19/04C12P2201/00C12P2203/00
Inventor 程峥杨仁党周晓明
Owner SOUTH CHINA UNIV OF TECH
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