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Culture medium good in hematopoietic stem cell adherence

A technology for hematopoietic stem cells and culture medium, which is applied in the field of culture medium with good adherence of hematopoietic stem cells, can solve the problems of residue, poor cell adhesion, pollution, etc., and achieves the effects of increasing the number, reducing the number of dead cells and increasing the growth rate.

Inactive Publication Date: 2016-11-23
ANHUI HUIEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The stem cell culture medium in the prior art often uses fetal bovine serum. Due to the use of animal-derived fetal bovine serum, the stem cell culture medium is mixed with non-human substances, resulting in residue or pollution, and the existing stem cell culture medium has cell stickers. The wall is poor, the cell proliferation rate is not ideal, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A culture medium with good adherence of hematopoietic stem cells proposed by the present invention includes RPMI-1640 medium; also includes: hyaluronic acid 5 μg / L, trehalose 3.8g / L, ethanolamine 20mg / L, potassium bicarbonate 1g / L L, methionine chelated selenium 6μg / L, β-mercaptoethanol 5μg / L, transferrin 2g / L, stem cell factor 260mg / L, placental growth factor 50mg / L, fibroblast growth factor 50mg / L, glutathione 80mg / L, vitamin 110mg / L, plant extract 180mg / L.

Embodiment 2

[0016] A medium with good adherence of hematopoietic stem cells proposed by the present invention includes RPMI-1640 medium; it also includes: hyaluronic acid 8 μg / L, trehalose 3.2g / L, ethanolamine 30mg / L, potassium bicarbonate 0.7g / L, methionine chelated selenium 9μg / L, β-mercaptoethanol 2μg / L, transferrin 3g / L, stem cell factor 200mg / L, placental growth factor 80mg / L, fibroblast growth factor 30mg / L, glutathione Peptide 100mg / L, vitamin 80mg / L, plant extract 220mg / L.

[0017] Vitamins include by weight: 6 parts of vitamin E, 14 parts of vitamin C, 3 parts of coenzyme Q10, 2 parts of choline chloride, 3 parts of thiamine, 4 parts of riboflavin, 1 part of niacin, 2 parts of pantothenic acid, pyridoxine Pyridoxine 1 part, cyanocobalamin 1.5 part, folic acid 0.5 part, biotin 0.8 part.

Embodiment 3

[0019] A medium with good adherence of hematopoietic stem cells proposed by the present invention includes RPMI-1640 medium; also includes: hyaluronic acid 6 μg / L, trehalose 3.6g / L, ethanolamine 22mg / L, potassium bicarbonate 0.9g / L, methionine chelated selenium 7μg / L, β-mercaptoethanol 4μg / L, transferrin 2.5g / L, stem cell factor 240mg / L, placental growth factor 60mg / L, fibroblast growth factor 45mg / L, glutathione Glycerin 85mg / L, vitamin 100mg / L, plant extract 190mg / L.

[0020] Vitamins include by weight: 8 parts of vitamin E, 12 parts of vitamin C, 5 parts of coenzyme Q10, 1 part of choline chloride, 6 parts of thiamine, 2 parts of riboflavin, 2 parts of niacin, 1 part of pantothenic acid, pyridoxine Pyridoxine 2 parts, cyanocobalamin 0.8 part, folic acid 1 part, biotin 0.5 part.

[0021] The plant extract comprises by weight: 2 parts of chlorogenic acid, 6 parts of resveratrol, 14 parts of jujube polysaccharide, 15 parts of ginsenoside and 24 parts of Polygonatum polysacch...

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Abstract

The invention discloses a culture medium good in hematopoietic stem cell adherence. The culture medium comprises an RPMI-1640 culture medium, 5-8mug / L of hyaluronic acid, 3.2-3.8g / L of trehalose, 20-30mg / L of ethanolamine, 0.7-1g / L of potassium bicarbonate, 6-9mug / L of methionine chelate selenium, 2-5mug / L of beta-mercaptoethanol, 2-3g / L of transferrin, 200-260mg / L of stem cell factors, 50-80mg / L of placental growth factors, 30-50mg / L of fibroblast growth factors, 80-100mg / L of glutathione, 80-110mg / L of vitamins and 180-220mg / L of plant extracts. The culture medium is good in cell adherence, rapid in proliferation rate, pathogen-resistant microbial action of the cultural medium is improved, components of animal origin are avoided, residues or pollution cannot be caused, and cost of the culture medium is lowered.

Description

technical field [0001] The invention relates to the technical field of stem cell culture medium, in particular to a culture medium with good adherence of hematopoietic stem cells. Background technique [0002] The stem cell culture medium in the prior art often uses fetal bovine serum. Due to the use of animal-derived fetal bovine serum, the stem cell culture medium is mixed with non-human substances, resulting in residue or pollution, and the existing stem cell culture medium has cell stickers. The wall is poor, and the cell proliferation rate is not ideal. Contents of the invention [0003] Based on the technical problems existing in the background technology, the present invention proposes a medium with good adherence of hematopoietic stem cells, which has good cell adherence, fast proliferation, improves the anti-pathogenic microorganism effect of the medium, and has no animal-derived components at the same time. It will not cause residue or pollution, reducing the co...

Claims

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Application Information

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IPC IPC(8): C12N5/0789
CPCC12N5/0647C12N2500/12C12N2500/24C12N2500/32C12N2500/38C12N2500/44C12N2500/46C12N2500/76C12N2501/10C12N2501/125C12N2501/90C12N2501/905C12N2501/998
Inventor 张正亮
Owner ANHUI HUIEN BIOTECH
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