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Preparation method of cell active element cultured from fetus skin and application thereof

A technology for culturing cells and cell activity, which is applied in the field of preparation of fetal skin cultured cytokines, can solve the problems of low specificity, poor biological activity, and inconspicuous effect of sheep fetal extracts, and achieves a simple and easy-to-operate preparation method, Easy to operate, the effect of increasing the moisture content of the skin

Inactive Publication Date: 2016-11-23
陈军 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is: the current sheep fetus extract has shortcomings such as low specificity, high immunogenicity, poor biological activity, and ineffective effects on the skin.

Method used

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  • Preparation method of cell active element cultured from fetus skin and application thereof

Examples

Experimental program
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Embodiment 1

[0033] A preparation method of fetal skin culture cytokinin, the specific process is as follows:

[0034] Step 1. Obtain the fetal sheep skin tissue, rinse the fetal sheep skin tissue with phosphate buffered saline containing penicillin for 3 times, soak it for disinfection, cut the skin tissue into small pieces and spread them on a plate, add protease to the plate Full-thickness cells were obtained by overnight culture, and the protease was neutral protease or trypsin;

[0035] In this step, the skin tissue is cut into 0.5-1mm 3 Small pieces; overnight culture conditions are 4 ℃ overnight culture;

[0036] Step 2: Carry out primary culture of the full-layer cells obtained by overnight culture in a culture dish. During the primary culture process, wash and replace the cell culture medium every three days; the conditions for primary culture are: add 0.5 ml of cells to the culture dish The cultivation is based on constant temperature cultivation at 37°C, and the volume fractio...

Embodiment 2

[0041] The purpose of this example is to provide an application method of the cytosine in the cosmetic of Example 1, and to disclose two cosmetic compositions with the cytosine of the present invention. The following compositions are all in parts by weight.

[0042] One of the cosmetic products consists of the following:

[0043] Sheep fetal skin active ingredient 4-6 parts, mannitol 3-7 parts, trehalose 3-7 parts, hyaluronic acid 0.02-1 part, water 80-90 parts.

[0044] Another cosmetic composition is as follows:

[0045] Sheep fetal skin active ingredient 4-6 parts, mannitol 3-7 parts, trehalose 3-7 parts, collagen 2-3 parts, water 80-90 parts.

Embodiment 3

[0047]This example is a comparative example of Example 1. Two sets of comparative examples are disclosed in this example, wherein the comparative example 1 is the cytokinin collected when the primary culture is in the adaptation period, and the comparative example 2 is the primary culture medium. Cytokines collected when only one or two viable cell types are present.

[0048] That is, the control example 1 of this embodiment is to use the cell active liquid prepared from the cell supernatant from the 1st to 10th day of primary culture to make cytokinin, and the control example 2 uses the cell of the 20th to 30th day of primary culture. The cell activation solution prepared from the supernatant is made into cytokinin.

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Abstract

The invention discloses a preparation method of a cell active element cultured from fetus skin and an application thereof, and solves the shortcomings of existing goat fetus extract such as low specificity, high immunogenicity, low bioactivity and non-obvious effect against skin. The preparation method comprises the following steps: (I) obtaining the fetus skin, and performing cleaning, disinfection and pre-culture on the fetus skin to obtain full-layer cell; (II) performing primary culture on the whole-layer cell by using a cell medium; and when the whole-layer cell adapts to the cultivation condition and more than two types of cells exist in the cell medium, collecting the cell supernate; and (III) performing membrane separation or / and chromatographic separation on the cell supernate, and collecting 2-100KD of bioactive substances to obtain the cell active element. The preparation method disclosed by the invention has the advantages of applicability to industrial production, low cost, remarkable effect and the like.

Description

technical field [0001] The present invention relates to a preparation method of cytosine, in particular to a preparation method of fetal skin culture cytosine, and provides the use of the cytosine obtained by the method. Background technique [0002] Sheep placenta is a general concept and generally includes two different products: the Extract of Sheep'sPlacental and the Extract of Goat's Embryo. [0003] Sheep placenta (ESP) is a biologically active substance extracted from sheep placenta, which is widely used in various cosmetics, medicines and health foods. [0004] Sheep embryonic element (EGE) is present in fetuses and sheep, and is widely used in various cosmetics, medicines and health foods after extraction. [0005] In 1912, Swiss scientist Professor Karl discovered for the first time that there is a substance in sheep embryo cells that can rejuvenate cells. This active substance can promote the increase in the number of cell divisions, and can force decayed or aged...

Claims

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Application Information

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IPC IPC(8): C12N5/073C12P21/02A61K8/64A61K8/65A61Q19/08
CPCC12N5/0603A61K8/64A61K8/65A61Q19/08C12P21/02
Inventor 陈军雁杰蔡学敏
Owner 陈军
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