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Bacterial strain for preventing and treating soil-borne fungal diseases of peanuts in continuous cropping and applications of bacterial strain

A soil-borne fungus and disease technology, applied in application, microorganism-based methods, chemicals for biological control, etc., can solve the problems of unstable field control effect, high application cost, large dosage, etc., and achieve significant yield increase effect. , low cost, and the effect of reducing the number of pathogenic bacteria

Active Publication Date: 2016-11-16
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in view of the fact that bio-control bacteria are living organisms, it is difficult to survive directly in organic fertilizers without intensive induction, resulting in unstable control effects in the field
In addition, the existing organic fertilizers for biological control generally use a large amount (>200 kg / mu), and the application cost is relatively high

Method used

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  • Bacterial strain for preventing and treating soil-borne fungal diseases of peanuts in continuous cropping and applications of bacterial strain
  • Bacterial strain for preventing and treating soil-borne fungal diseases of peanuts in continuous cropping and applications of bacterial strain
  • Bacterial strain for preventing and treating soil-borne fungal diseases of peanuts in continuous cropping and applications of bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The peanut rhizosphere soil of a piece of continuous cropping peanut field in the red soil area was collected for related experiments, and the specific steps were as follows:

[0034] (1) Soil sample treatment: prepare sterilized enrichment medium (ingredients: 2.5g of fine powder chitin, MgSO 4 ·7H 2 O 0.5g, K 2 HPO 4 0.7g, KH 2 PO 4 0.3g, FeSO 4 ·7H 2 (0.01g, distilled water 1000ml), sub-pack 20ml in a sterile Erlenmeyer flask, and get 1.0g of fresh soil sample into the Erlenmeyer flask, mix well, and cultivate at room temperature for 48h at 150r / min to obtain an enriched culture solution.

[0035] (2) Separation by coating: absorb 1mL filtrate and add 9.0mL sterile water, serially dilute 3 times to obtain a dilution of 10 -4 Gradiently dilute the sample solution, spread 0.02mL of the soil dilution on the plate separation medium, incubate at 28°C for 4-7d, observe and pick the bacterial strains that produce transparent circles.

[0036] (3) Cultivation and pur...

Embodiment 2

[0042] Embodiment 2: Bacteriostasis experiment to various soil-borne fungal pathogenic bacteria

[0043] Utilize preservation number to be the bacterial strain of CGMCC NO.11539 to carry out antibacterial experiment: first described bacterial strain is activated, inoculate pathogenic fungus earlier in the center of PDA flat plate, punch the edge of pathogenic bacteria colony with the hole puncher of diameter 5mm to make bacterium cake, and inoculate To the center of the PDA plate, and then inoculate the bacterial strain to be tested about 2.5cm away from the pathogenic bacteria around the petri dish. Each strain of bacteria has three replicates, cultured at 28°C for one week, observe whether there is an inhibition zone, and use the cross method to measure the diameter of the pathogenic fungal colony , to calculate the inhibition rate. The selected pathogens are: Rhizoctonia solani, Fusarium solani, Fusarium oxysporum f.sp.diath, Verticilliumdahliae Kleb, such as figure 2 — ...

Embodiment 3

[0046] Embodiment 3: Greenhouse living body control experiment

[0047] Activate the peanut root rot pathogen (Fusarium solani) in PDA medium and culture it at 28°C for 7 days, wash the spores of the pathogenic bacteria on the medium with sterile water, filter the bacterial solution with sterilized filter paper to remove the hyphae, and the pathogenic bacteria will Dilute the filtrate with appropriate distilled water to a spore concentration of 1×10 7 Individual / mL suspension, set aside.

[0048] The biocontrol strains for testing were added into culture medium without fungal hyphae inducer (3% beef extract, 8% peptone) for liquid fermentation (26°C, 72h, 200rpm), and then inoculated with decomposed pig manure in a proportion of 5%. Compost the substrate, place it in an incubator (28°C) and cultivate it for 5 days, and use it as bacterial agent A for future use.

[0049] Simultaneously according to above-mentioned same method, just add inactivated fungal mycelia inducer (3%)...

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Abstract

The invention relates to a bacterial strain for preventing and treating soil-borne fungal diseases of peanuts in continuous cropping and a fungicide of the bacterial strain, wherein the bacterial strain is Pseudomonas protegens, and is assigned with the accession number of CGMCC No.11539. The 16s rRNA base sequence of the bacterial strain is shown as SEQ ID No:1. The bacterial strain has high activity in generating chitinase (degrading cell walls of pathogenic bacteria hyphae), and has the obvious degrading capacity for various soil-borne fungous pathogenic bacteria mycelia, such as fusarium, rhizoctonia and verticillium chlamydosporium. For the fungicide provided by the invention, the seed dressing use amount is only 20-30 kg / mu normally, the fungicide can be used for effectively preventing and treating the soil-borne fungous diseases, such as root rot, damping off and southern blight frequently occurring in peanuts continuously planted in dry fields, the control effect achieves 55% or above, in addition, the bacterial strain has the advantages of small use amount, wide antimicrobial spectrum, obvious yield-increasing effect and the like, so that the influence of the soil-borne diseases on the yield loss of the peanuts in continuous cropping is effectively alleviated.

Description

technical field [0001] The invention relates to a bacterial strain for continuous cropping peanut soil-borne fungal diseases and a preparation method thereof, belongs to the technical field of agricultural intensive production, and is specially used for overcoming the problem of high occurrence of continuous cropping peanut soil-borne fungal diseases. Background technique [0002] Peanut is an important oil crop in my country, and the annual sown area accounts for about 35% of the total sown area of ​​oil crops. The red soil hilly area in the south is one of the main peanut producing areas in my country. Compared with the main producing areas in North China, the peanut production in the red soil area in the south is relatively low (the average yield is only about 60% of that in North China). It is related to climate conditions, soil fertility, etc., and also has a certain relationship with the serious obstacles to peanut continuous cropping in the southern red soil area. Due...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/38
CPCA01N63/00C12N1/20C12N1/205C12R2001/38
Inventor 李孝刚王兴祥李忠佩丁昌峰周志高
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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