Plasmodiophora brassica woron inoculating nutrition medium and preparation method thereof

Abstract

The invention discloses a plasmodiophora brassica woron inoculating nutrition medium and a preparation method thereof. The plasmodiophora brassica woron inoculating nutrition medium consists of the following components according to a volume ratio: peat soil, vermiculite and perlite at 1 to (0.8-1.2) to (0.8-1.2). The invention discloses a preparation method of the plasmodiophora brassica woron inoculating nutrition medium, wherein the method comprises the following steps: uniformly mixing the peat soil, the vermiculite and the perlite according to the volume ratio, sterilizing and processing the nutrition medium and sub-packaging the nutrition medium in round plastic nutrition pots. Simultaneously, the invention discloses a nutrition medium applied in plasmodiophora brassica woron expanding propagation, wherein the component of the nutrition medium is 100% quartz sand. The nutrition medium disclosed by the invention, as an ideal plasmodiophora brassica woron inoculating nutrition medium, can improve a host seedling incidence rate to 100% and a disease rate to 69.6 ; and the nutrition medium, which is high in incidence rate and stable in disease incidence, is applicable to the identification of plasmodiophora brassica woron physiological race and the identification and screening of clubroot-resistant breeding materials.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a nutrient matrix for inoculating Plasmodium bacterium and a preparation method thereof. Background technique [0002] Clubroot is a disease of cruciferous vegetables caused by Plasmodiophora brassicae Woron. At present, it is generally believed that it includes two generations: dormant spores, protoplasts, and three stages: the survival stage in the soil, the root hair infection stage, and the cortex infection stage (Kageyama & Asano, 2009). The conditions that can promote the germination of these dormant spores are not very clear, but they are easy to successfully infect the host under the conditions of ambient temperature 6°C to 27°C and acidic pH value (less than 6.0) (Karling, 1968). When a susceptible host is present and the soil is nearly saturated with water, dormant spores germinate to release primary zoospores, which have a pair of prefronted flagell...

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Problems solved by technology

[0004] The object of the present invention is to provide a kind of nutrient matrix for inoculation of clubroot fungus and its preparation method, aiming to solve the problem that the operation procedure of the inoculation and identification method of clubroot fungus is relatively complex and strict, and it is easy to form cross-contamination and inoculation between different bacterial sources. The problem of instability of post-plant disease

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