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A kind of rapid detection method of cyanide ion

A detection method, the technology of cyanide ion, which is applied in the field of nanotechnology and analysis and detection, can solve the problems of short half-life of cyanide ion and analysis error, etc., and achieve the effect of fast detection speed, easy operation and good social value

Active Publication Date: 2019-01-18
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The half-life of cyanide ion is short, complex pretreatment or long detection time will lead to analysis errors, so real-time and rapid detection of cyanide ion is particularly important

Method used

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  • A kind of rapid detection method of cyanide ion
  • A kind of rapid detection method of cyanide ion
  • A kind of rapid detection method of cyanide ion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Different DNA sequences are used in the construction of nanocomposite probes and their responses to cyanide ions

[0042] Prepare the solution required for the preparation of nanocomposite probes: dissolve the dry powder containing different DNA sequences of the same length in water to obtain a 10 μM stock solution, prepare a 50 μM EBMVC-B solution, and prepare 2 mM copper sulfate (CuSO 4 ) solution, prepare 100mM sodium ascorbate (C 6 h 7 NaO 6 ) solution, prepare 10 mM MOPS buffer solution (pH=7.8), and place it in a refrigerator at 4°C until use.

[0043] (1) Add 480 μL of MOPS buffer solution (10 mM, pH=7.8) into an EP tube with a volume of 1.5 mL; then add 5 μL of DNA solution (10 μM) and 5 μL of EBMVC-B dye solution (50 μM), shake well and let stand 2 minutes; then add 5 μL of copper sulfate solution (2mM) and 5 μL of sodium ascorbate solution (100mM), shake well, and let stand for 2 minutes; directly measure and record the fluorescence of each group ...

Embodiment 2

[0046] Example 2 Feasibility Analysis of Nanocomposite Probes for Cyanide Ion Detection

[0047] By single variable method, the influence of each reaction component on the fluorescence signal was systematically investigated.

[0048] (1) Add 480 μL of MOPS buffer solution (10 mM, pH=7.5) into an EP tube with a volume of 1.5 mL;

[0049] (2) Add 5 μL of the optimally obtained double-stranded d(AT) 20 solution (10 μM);

[0050] (3) Add 5 μL of EBMVC-B dye solution (50 μM), shake well, and let stand for 2 minutes;

[0051] (4) Add 5 μL of copper sulfate solution (2 mM);

[0052] (5) Add 5 μL of sodium ascorbate solution (100 mM), shake well, and let stand for 2 minutes. Sodium ascorbate is abbreviated as SA in the drawings of this invention.

[0053] (6) Finally, add 5 μL of cyanide ion solution (2 mM) and shake well.

[0054] Test and record the fluorescence spectra of the above steps respectively.

[0055] Analysis of results: from image 3 It can be seen from the fluore...

Embodiment 3

[0056] Example 3 Kinetic Analysis of Nanocomposite Probes for Cyanide Ion Detection

[0057] Set the parameters of the fluorescence spectrophotometer, excitation wavelength=450nm, emission wavelength=550nm, and perform time scanning.

[0058] (1) Add 480 μL of MOPS buffer solution (10 mM, pH=7.8) into an EP tube with a volume of 1.5 mL, and scan for 100s-200s;

[0059] (2) Then add 5μL d(AT) 20 Solution (10μM), shake well, scan for 100s-200s;

[0060] (3) Add 5 μL of EBMVC-B dye solution (50 μM), shake well, and scan for 100s-200s;

[0061] (4) Then add 5 μL of copper sulfate solution (2mM), shake well, and scan for 100s-200s;

[0062] (5) Add 5 μL of sodium ascorbate solution (100mM), shake well, and scan for 100s-200s;

[0063] (6) Finally, add 5 μL cyanide ion solution (2 mM), shake well, and scan for 100s-200s.

[0064] Analysis of results: from Figure 4 It can be seen from the time scanning results of the MOPS buffer itself that there is no fluorescence signal; add...

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Abstract

The invention discloses a quick detection method for cyanide ions. The quick detection method is characterized in that by studying the optical mutual action of DNA (deoxyribose nucleic acid)-copper nanoparticles (DNA-CuNPs) and fluorescent dye molecules, the fluorescence of a DNA-embedded type dye EBMVC-B can be efficiently extinguished by CuNPs, the CuNPs can be quickly etched by the cyanide ions (CN<->), the fluorescence is restored, and the process can be completed in several seconds; the quick fluorescence analysis and detection of the cyanide ions is realized. After proofing by results, the quick detection method has the advantages that the specificity is high, the sensitivity is good, the operation is simple and convenient, the economy and practicality are realized, and the like; the detection speed is quick, and the error caused by short CN- half-life period, and complicated pretreatment process or long detection time can be overcome; the detection of CN<-> in an actual water sample and the florescence imaging analysis of CN<-> in edible plant tissues are realized; the original innovativeness, and good social value and application prospect of the method are realized.

Description

technical field [0001] The invention belongs to the field of nanotechnology and analysis and detection, and relates to the construction of a nanocomposite probe based on DNA-copper nanoparticles and a new method for detecting and analyzing cyanide ions by using the same. Background technique [0002] Cyanide ion is a highly toxic anion and is highly lethal. Because it widely exists in human production and life in different forms, such as thousands of plants and even crops, industrial wastewater, etc. Not only that, many cyanide-containing complexes are found in complex environments, such as linamarin in cassava, which is hydrolyzed by gastric acid to generate free hydrocyanic acid, resulting in toxicity. In the event of cyanide ion poisoning, mild symptoms of nausea, vomiting, diarrhea, dizziness, severe breathing difficulties, rapid heartbeat, dilated pupils, and even coma, and finally convulsions, shock, and death due to respiratory failure. It can also cause chronic dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6458
Inventor 杨荣华卿志和侯丽娜朱栎璇杨盛
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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