Method for simultaneous separation of T and B lymphocytes in peripheral blood
A technology of simultaneous separation of B lymphocytes, applied in the field of cell culture, can solve the problems of low cell acquisition rate, impact on research, and low efficiency of separation technology cell separation, and achieve the effect of fast cell proliferation and high activity
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[0041] 1. Handle nylon wool and install nylon wool column tube
[0042] 1 pack of 2 grams of nylon wool is taken out and shredded as much as possible. Then use deionized water to boil the nylon wool for 20 minutes, then let it cool for 20 minutes, repeat 6 times in total, and then soak it in 0.2mol / l HCL for 24 hours. The purpose is to make the nylon wool as fluffy and stretchable as possible after repeated boiling and soaking in acid. To achieve the best extension effect, it can better absorb B lymphocytes. Then put the boiled nylon wool into the oven to dry for about 4 hours. After the nylon hair is completely dry, tear it carefully into a single fiber state, the finer the better. Then these nylon hairs are carefully filled into a 5ml disposable syringe barrel (the syringe barrel used here does not include the push column inside), about 0.5 grams of nylon hair can hold a column, and the volume is about 4 Milliliters, pay attention to the moderate tightness, and autoclave ...
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