Method for preparing functional gingko fermented beverage
A fermented beverage and functional technology, which is applied in the field of preparation of functional ginkgo fermented beverages, can solve the problems of narrow development and utilization of ginkgo, and achieve the effects of low production cost, stable strain quality, and short strain formation cycle
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Embodiment 1
[0023] 1. Preparation of ginkgo enzymatic solution: Weigh 30g of ginkgo powder, add water and stir at a ratio of 1:10 to solid-liquid ratio; Ginkgo powder is gelatinized and liquefied at the same time, stirred properly, taken out after 20 minutes in water bath; cooled to about 55°C, adding glucoamylase at an amount of 1350U / g ginkgo powder, and adding neutral protease at an amount of 800U / g ginkgo powder, Shake the water bath at 55°C for 3 hours, take it out, centrifuge and filter to obtain the ginkgo enzymatic hydrolyzate, boil and sterilize for 30 minutes and set aside.
[0024] 2. Activation of bacterial classification: insert the preserved bacterial classification Gluconacetobacter L4 into the acetic acid bacteria culture solution (glucose 100g / L, yeast extract 10g / L) with inoculum size 5%, at 30 ℃, 150rpm rotating speed Activation in a shaker incubator for 2 days.
[0025] 3. Preparation of seed liquid: Inoculate the activated Gluconacetobacter L4 in Ginkgo biloba hydrol...
Embodiment 2
[0028] 1. Preparation of ginkgo enzymatic solution: Weigh 30g of ginkgo powder, add water and stir at a ratio of 1:10 to solid-liquid ratio; Ginkgo powder is gelatinized and liquefied at the same time, stirred properly, taken out after 20 minutes in water bath; cooled to about 55°C, adding glucoamylase at an amount of 3900U / g ginkgo powder, and at the same time adding 1600U / g ginkgo powder, at 55°C Take it out after oscillating in a water bath for 3 hours, centrifuge and filter to obtain the ginkgo enzymatic hydrolyzate, boil and sterilize for 30 minutes and set aside.
[0029] 2. Activation of bacterial classification: insert the preserved bacterial classification Gluconacetobacter L4 into the acetic acid bacteria culture solution (glucose 100g / L, yeast extract 10g / L) with inoculum size 5%, at 30 ℃, 150rpm rotating speed Activation in a shaker incubator for 2 days.
[0030] 3. Preparation of seed solution: Inoculate the activated Gluconacetobacter L4 in Ginkgo biloba hydroly...
Embodiment 3
[0033] 1. Preparation of ginkgo enzymatic solution: Weigh 30g of ginkgo powder, add water and stir at a solid-to-liquid ratio of 1:10; Ginkgo powder is gelatinized and liquefied at the same time, stirred properly, taken out after 20 minutes in water bath; cooled to about 55°C, adding glucoamylase at an amount of 3900U / g ginkgo powder, and at the same time adding 1600U / g ginkgo powder, at 55°C Take it out after oscillating in a water bath for 3 hours, centrifuge and filter to obtain the ginkgo enzymatic hydrolyzate, boil and sterilize for 30 minutes and set aside.
[0034]2. Activation of strains: Insert the preserved strain Gluconacetobacter L4 into the acetic acid bacteria culture solution with an inoculum size of 5%, and insert Dekkerya heterozygous yeast into the yeast culture solution with an inoculum size of 2%, at 30°C, 150rpm Activation 2d in a shaker incubator at high speed.
[0035] 3. Preparation of seed solution: Inoculate the activated Gluconacetobacter L4 and Dek...
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