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Preparation method for extracellular matrix (ECM) composite biological patch

A biological patch and extramatrix technology, applied in medical science, prosthesis, etc., can solve the problems of limited increase in tensile strength of products, serious loss of biological materials, and economic impact, and achieve material saving and great economic benefits. , good uniformity effect

Inactive Publication Date: 2016-10-26
SHANGHAI HONGCHUANG MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Some ECM biomaterial composite overlapping methods have been reported (such as CN 103877619 A), but the excessive material overlap (greater than 1cm) is likely to cause obvious local unevenness of the product, and the process of composite molding can only increase the tensile strength of the product. The loss of biological materials is also serious, and large-scale production in the future will have a certain impact on economic benefits

Method used

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  • Preparation method for extracellular matrix (ECM) composite biological patch
  • Preparation method for extracellular matrix (ECM) composite biological patch
  • Preparation method for extracellular matrix (ECM) composite biological patch

Examples

Experimental program
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Effect test

Embodiment 1

[0031] (1) Preparation of SIS or UBM: SIS or UBM material is obtained after mechanical treatment of pig small intestine or bladder, and SIS or UBM is obtained after disinfection and cleaning with oxyacetic acid + ethanol.

[0032] A. Cut the SIS longitudinally along the intestine, and cut the SIS into SIS units with a longitudinal length of 5-30cm along the transverse direction;

[0033] B. UBM does not need to be cut, and goes directly to the next step (the rest of the methods are the same as SIS).

[0034] (2) Production of vacuum lamination device: connect a vacuum pump with a rubber tube to one end of a pair of nozzle suction filter bottles, and place the suction filter bottles in a foam box with dry ice for use.

[0035] (3) Take an A4 paper size (30*21cm) phenolic plastic material flat plate evenly distributed with many through holes (patterns are square, diamond, circle, etc.)] and lay it on the table, and lay a layer of medical sand on the flat plate Cloth or not, lay...

Embodiment 2

[0043] Prepare the semi-finished product in (9) according to the method of Example 1, freeze-dry it with a freeze-drying machine (-50° C., under the condition of vacuum 50 μbar, freeze-drying for 16 hours), after freeze-drying, the intermediate product after freeze-drying is lyophilized by means of a seam tenacity machine. Suture with absorbable thread.

Embodiment 3

[0045] Take an A4 paper size phenolic plastic board, use SIS to prepare a composite biological patch according to the method of Example 1, wherein the A layer and the B layer are a total of 6 layers (the length of the overlapping part is 0.8cm), and after lamination, it is placed in a freeze dryer (-50 ℃, under the condition of vacuum 50μbar, freeze-dried for 16 hours) to obtain the finished SIS biological patch. (The tensile strength measured with a tensile tester was 52N).

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Abstract

The invention relates to a preparation method for an ECM composite biological patch. Specifically, the preparation method comprises the following steps: partially overlapping a plurality of ECM units along a transverse or longitudinal direction to form a layer and partially overlapping a plurality of ECM units along a direction perpendicular to the layer in sequence so as to form a second layer; repeating the previous step as needed until a desired layer number is obtained; and then subjecting the laid material to vacuumized lamination so as to obtain the composite biological patch. The composite biological patch prepared by using the method has improved tensile strength and can reduce consumption of ECM.

Description

technical field [0001] The invention relates to a preparation method of an extracellular matrix composite biological patch. Background technique [0002] The extracellular matrix (ECM) is a complex dynamic system, which is not only a biological scaffold necessary for maintaining tissues and organs, preventing tissues and organs from dissolving into a paste, but also releasing important signaling molecules and regulating the body. Do self-healing. ECM biomaterials currently on the market mainly include small intestinal submucosa (SIS), bladder mucosal basement membrane (UBM), amniotic membrane, dermis, pericardium, peritoneum, etc. It has the advantages of good biocompatibility with human body and no foreign body reaction. [0003] Since diseases such as hernia reconstruction and dura mater repair have certain requirements on the tensile strength and size of repair materials, the tensile strength and size of monolithic ECM (such as SIS and UBM) biomaterials are far from mee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/40
CPCA61L27/3633A61L27/3687A61L27/3691
Inventor 周宁辉尹华清
Owner SHANGHAI HONGCHUANG MEDICAL TECH CO LTD
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