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New sesquiterpene compound and application thereof in anaemia treatment

A compound and composition technology, applied in the field of new sesquiterpene compounds, can solve problems such as chemical components that have not been separated

Inactive Publication Date: 2016-10-12
李月升
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are many studies on the chemical constituents of Astragalus mongolica, but no chemical constituents with significant therapeutic effect on anemia have been isolated from Astragalus mongolica

Method used

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  • New sesquiterpene compound and application thereof in anaemia treatment
  • New sesquiterpene compound and application thereof in anaemia treatment
  • New sesquiterpene compound and application thereof in anaemia treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Compound (I) Separation Preparation and Structure Confirmation

[0027] Preparation method: (a) Grind the dry root (2kg) of Astragalus mongolica, extract with 80% ethanol under heat reflux (15L×3 times), combine the extracts, concentrate until no alcohol smell (3L), and successively add petroleum ether (3L ×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol (3L×3 times) were extracted to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b) In the step (a), the ethyl acetate extract was removed with D101 macroporous resin, and 8 column volumes were first eluted with 15% ethanol, and then 14 column volumes were eluted with 75% ethanol, and the 75% eluent was collected. Concentrate under reduced pressure to obtain a 75% ethanol elution concentrate; (c) in step (b), the 75% ethanol elution concentrate is separated with normal phase silica gel, and the volume ratio is 90:1 (10 column volumes), 50: The dichl...

Embodiment 2

[0031] Example 2: Therapeutic effect on hemolytic anemia

[0032] In this example, 2% phenylhydrazine was used to prepare an anemic mouse model, and the anti-anemia effect of the drug on increasing the number of red blood cells and increasing the content of hemoglobin was observed.

[0033] 1. Materials and methods

[0034] 1.1 Animals

[0035] Kunming mice, weighing 18-22 g, half male and half male, were provided by Experimental Animal Center of Henan Province.

[0036] 1.2 Reagents and samples

[0037] Compound (I) is self-made, and the preparation method is shown in Example 1. Phenylhydrazine hydrochloride was purchased from Sigma.

[0038] 1.3 Instruments

[0039] The blood cell analyzer NIKON KOHDEN Celltac is produced in Japan; the pH meter HANNApH211 is produced in Italy.

[0040] 1.4 Mouse grouping and model preparation

[0041] They were randomly divided into 3 groups with 12 rats in each group, which were normal control group, model control group and compound ...

Embodiment 3

[0058] Example 3: Therapeutic effect on aplastic anemia

[0059] In this example, a mouse model of aplastic anemia was prepared by combining subcutaneous injection of acetylphenylhydrazine with intraperitoneal injection of cyclophosphamide, and observed that the drug increased the count of platelets (PLT), increased serum pluripotent colony-stimulating factor (Multi-CSF), Erythropoietin (EPO) content and other aspects of the role of anti-aplastic anemia.

[0060] 1. Materials and methods

[0061] 1.1 Animals

[0062] ICR mice, half male and half female, weighing 18g-22g, were provided by the Animal Center of Jilin University.

[0063] 1.2 Reagents and samples

[0064] Compound (I) is self-made, and the preparation method is shown in Example 1. Compound donkey-hide gelatin pulp: product of Shandong Dong'e donkey-hide gelatin Co., Ltd.; multipotent colony-stimulating factor (Multi-CSF) detection kit and erythropoietin (EPO) detection kit are all products of American R&D comp...

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Abstract

The invention discloses a new sesquiterpene compound and an application thereof in anaemia treatment, and provides a structure of the compound, a medicinal composition containing the compound, a preparation method of the compound, and applications of the compound and the medicinal composition. The compound is reported for the first time, is a sesquiterpene compound with a novel structure, and can be obtained through separation and purification after being extracted from Membranous Milkvetch Root Mongolian Milkvetch Root. The compound has a treatment effect on hemolytic anaemia or aplastic anemia, can be processed to develop hemolytic anaemia or aplastic anemia treatment drugs, and provides a new treatment scheme for clinic hemolytic anaemia and aplastic anemia treatment.

Description

technical field [0001] The invention belongs to the technical field of medicines and relates to a new natural product, in particular to a new sesquiterpene compound isolated from Astragalus mongolica and its application in anemia. Background technique [0002] Astragalus mongolica belongs to Rosaceae, leguminous plants, and is a unique plant in China. Distributed in Heilongjiang, Jilin, Inner Mongolia, Hebei, Shanxi. [0003] The crude drug Astragalus mongolica is the root of the dicotyledonous herb leguminous plant Astragalus mongolica. Root cross-section, the cork layer is multi-row cork cells. The cortex is 2-3 rows of tangentially elongated cells. Phloem fibers are bundled or scattered individually, fiber wall is thick, non-lignified or slightly lignified, arranged alternately with sieve tube groups and phloem parenchyma cells; phloem rays are 2-5 rows of cells wide, often curved on the outside, with cracks ; Near the cortex, cork tissue rings (longitudinally tubular...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C35/37C07C29/74C07C29/76A61K31/045A61P7/06
CPCC07C35/37C07C29/74C07C29/76
Inventor 李月升
Owner 李月升
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