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Recovery method of protein in high concentration protein wastewater

A protein wastewater and recovery method technology, applied in the field of protein separation, can solve the problems of low treatment cost, high protein removal rate, low protein removal rate, etc., and achieve the effect of strong precise adjustment ability, high product added value, and improved removal rate

Inactive Publication Date: 2016-10-12
OCEAN UNIV OF CHINA
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Problems solved by technology

[0007] In order to overcome the disadvantages of low protein removal rate, high subsequent treatment cost after wastewater treatment, and poor controllability of wastewater treatment process due to the inability to continuously remove proteins at different isoelectric points in the treatment process of high-concentration protein wastewater in the existing industrialization technology, the present invention Provide a method for removing protein in high-concentration protein wastewater that can be implemented on the industrial site. Using this method to treat high-concentration protein wastewater not only has a high protein removal rate, but also realizes the fractional precipitation and recovery of protein through low-cost and precise pH adjustment in the wastewater treatment process. , the recovered protein can be obtained as a by-product, and the cost of subsequent treatment after wastewater treatment is low

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  • Recovery method of protein in high concentration protein wastewater

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Effect test

Embodiment 1

[0040] Select a kind of waste water produced after pollock surimi processing. The initial pH value of the surimi wastewater is about 7.1. According to the analysis of protein components, the protein concentration of the waste water after centrifugation pretreatment is 8.9±0.8mg / mL, and the isoelectric point Relatively densely concentrated in the vicinity of 5.5 and 4.3, according to the analysis results, it is divided into two levels according to the isoelectric point of the protein from large to small.

[0041] First-level isoelectric precipitation, the nitrogen pressure of the carrying tank is maintained at 0.5MPa, the temperature of the carrying tank is 50°C, and the acid-adjusting tank is kept at room temperature. When the cycle carrying time is 30 minutes, the pH of the acid-adjusting tank reaches around 5.5. After the gas in the acid adjustment tank was emptied, let it stand for precipitation for 20 minutes, and then centrifuge the high-concentration protein wastewater to...

Embodiment 2

[0044] Select a kind of wastewater produced after the processing of blue cod surimi. The initial pH value of the surimi wastewater is about 7.1. According to the analysis of protein components, the protein concentration of the wastewater after centrifugation pretreatment is 8.5±1.1mg / mL, and the isoelectric point They are respectively concentrated in the vicinity of 6.0 (there are three bands of 6.6, 6.3, and 5.8) and 4.3 (two bands of 4.4 and 4.2). According to the analysis results, the isoelectric point of the protein is adjusted from large to small by using a precise fractional precipitation scheme. It is determined to be divided into 5 levels, namely: 6.6, 6.3, 5.8, 4.4 and 4.2.

[0045] For first-stage isoelectric precipitation, the nitrogen pressure of the carrying tank is kept at 1.0MPa, the temperature of the carrying tank is 25°C, and the acid-adjusting tank is kept at normal temperature. When the cycle carrying time is 15 minutes, the pH of the acid-adjusting tank rea...

Embodiment 3

[0051] Select a kind of wastewater produced after processing blue cod surimi. The initial pH value of the surimi wastewater is about 7.1. According to the analysis of protein components, the protein concentration of the wastewater after centrifugation pretreatment is 8.5±1mg / mL, and the isoelectric points are respectively Concentrate around 6.0 (there are three bands of 6.6, 6.3, and 5.8) and 4.3 (two bands of 4.4 and 4.2). According to the analysis results, the isoelectric point of the protein is determined from the largest to the smallest by using a quick fractional precipitation scheme Divided into 3 levels, namely: 6.5, 5.8 and 4.3.

[0052] For first-stage isoelectric precipitation, the nitrogen pressure of the carrying tank is maintained at 1.0 MPa, the temperature of the carrying tank is 30°C, the acid-adjusting tank is kept at room temperature, and the pH of the acid-adjusting tank reaches 6.5 when the cycle carrying time is 30 minutes. After the acid-adjusting tank wa...

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Abstract

The invention provides a recovery method of protein in high concentration protein wastewater. Nitrogen circulation carries acetic acid into high concentration protein wastewater to adjust pH change step by step to different protein isoelectric points (pI), thus achieving graded isoelectric precipitation and recovery of protein from wastewater. The method provided by the invention adopts carry of acetic acid by pressurized nitrogen circulation as an acidic adjustment means, makes the pH value adjustment mild and controllable, can precipitate out proteins with different isoelectric points under different operation conditions respectively, thereby achieving the purpose of efficient and graded removal of protein from high concentration protein wastewater by protein isoelectric point precipitation technique.

Description

technical field [0001] The invention relates to a method for separating protein from waste water, especially recovering protein from high-concentration protein waste water. Background technique [0002] During the processing of some protein-rich animal and plant raw materials such as meat, fish and soybeans, protein-rich wastewater is often produced. Especially in the processing of minced meat and surimi, there is a large amount of protein-rich wastewater with high organic content and high COD (chemical oxygen demand) value, the most important of which is a large amount of water-soluble protein. Using traditional A / O process (anaerobic and aerobic process) treatment is difficult to meet the national standard, and the cost of wastewater treatment is quite high; the use of aerobic biological treatment consumes a lot of energy, while the conventional anaerobic biological treatment requires a long period of hydraulic power to reach the methanation stage The residence time, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F9/04C07K1/30
CPCC02F9/00C02F1/38C02F1/52C02F1/66C02F2209/03C02F2209/06C07K1/30
Inventor 齐祥明王路毛相朝林洪
Owner OCEAN UNIV OF CHINA
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