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Application of fingerprint atlas composed of microRNAs in gastric cancer

A technology for gastric cancer and gastric cancer cells, applied in the field of bioengineering and detection, and biomedicine, can solve problems such as survival rate hovering at 20%, metastasis, and patient recurrence

Active Publication Date: 2016-10-05
SHANGHAI XIANGQIONG TECHNOLOGY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the past half a century, people have invested a lot of energy in the basic and clinical research of gastric cancer, have a certain understanding of the potential carcinogenic factors of gastric cancer (such as Helicobacter pylori), effective screening of high-risk groups of gastric cancer and the improvement of early diagnosis rate, surgical operation With the application of multiple treatments, the prognosis of gastric cancer patients has been significantly improved, but the overall 5-year survival rate is still hovering at 20%.
In addition, most gastric cancer patients are still difficult to detect early. According to our previous research, 60% of gastric cancer patients have lymph node metastasis when they are first diagnosed, and more than 5% of patients have organ metastasis. Even if radical resection is performed, most patients will still develop recurrence and metastasis

Method used

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  • Application of fingerprint atlas composed of microRNAs in gastric cancer
  • Application of fingerprint atlas composed of microRNAs in gastric cancer
  • Application of fingerprint atlas composed of microRNAs in gastric cancer

Examples

Experimental program
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Effect test

preparation example Construction

[0126] The preparation of the miRNA chip can adopt conventional manufacturing methods of biochips known in the art. For example, if the solid phase carrier is a modified glass slide or silicon wafer, and the 5' end of the probe contains amino-modified poly-dT strings, the oligonucleotide probe can be formulated into a solution, and then spotted with a spotting instrument. The miRNA chip of the present invention can be obtained by arranging in a predetermined sequence or array on a modified glass slide or a silicon chip, and then fixing it by standing overnight. If the nucleic acid does not contain amino modification, its preparation method can also refer to: "Gene Diagnosis Technology-Non-Radioactive Operation Manual" edited by Wang Shenwu; J.L.eris i, V.R.Iyer, P.O.BROWN.Exploring the metabolic and genetic control of gene express ion on genomic scale. Science, 1997; 278:680 and Ma Liren, edited by Jiang Zhonghua. Biochip. Beijing: Chemical Industry Press, 2000, 1-130.

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Embodiment 1

[0147] The collection of embodiment 1 sample and information analysis

[0148] The samples in this study were paraffin wax samples collected and archived by Changzheng Hospital from 2011 to 2013. All samples were collected with personal consent, and according to the protocol approved by the hospital's ethics committee, pathological diagnosis results were confirmed by more than two pathologists. All cases were adenocarcinoma, and tumor tissue or mucosal tissue accounted for more than 85% of the samples. The clinical data of the patients included age of onset, gender, tumor location, lymph node metastasis, depth of invasion, tissue differentiation, and tumor TNM staging (AJCC Seventh Edition), etc.

[0149] A total of 93 samples participated in this experiment, including 40 gastric cancer samples, 32 normal tissue samples, with a median age of 59.5 years, and 21 gastric cancer cell lines. Samples were removed from patients and stored at -80°C. The samples are divided into two...

Embodiment 2

[0150] Example 2 total RNA extraction

[0151] Cut 7-8 5-micron or 4 10-micron tissue fragments from paraffin FFPE tissue, and remove excess paraffin around the tissue. The remaining tissues were stored at room temperature.

[0152] After the cut fragments were homogenized, the paraffin samples were first deparaffinized according to the instructions of Deparaffinization solution (Qiagen, 19093); and then the total RNA was extracted according to the instructions of miRNeasy FFPE kit (Qiagen, 217504).

[0153] The quality was detected by electrophoresis, the OD260nm and OD280nm were measured by ultraviolet spectrophotometry, and the RNA concentration was calculated. Store at -80°C.

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Abstract

The invention relates to an application of a fingerprint atlas composed of microRNAs in diagnosis and treatment of human gastric cancer. The fingerprint atlas, which is composed of a plurality of microRNAs, can quite effectively distinguish gastric cancer tissues from tissues (normal tissues) adjacent to the gastric cancer tissues; and the fingerprint atlas, which is high in sensitivity and strong in specificity, is applicable to the effective diagnosis of the gastric cancer.

Description

technical field [0001] The invention relates to the technical fields of biomedicine, bioengineering and detection. Specifically, the present invention discloses the use of a group of small RNA (miRNA; microRNA) fingerprints in the diagnosis of human gastric cancer. Background technique [0002] At present, China has become a high-incidence area of ​​gastric cancer in the world, with 400,000 new cases and 300,000 deaths every year, more than twice the world average. And there are obvious regional differences in the incidence of gastric cancer. The incidence of gastric cancer in the northwest and eastern coastal areas of my country is significantly higher than that in the south. The age of onset is more than 50 years old, and the ratio of male to female incidence is 2:1. In the past half a century, people have invested a lot of energy in the basic and clinical research of gastric cancer, have a certain understanding of the potential carcinogenic factors of gastric cancer (su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/63C40B40/06C12Q1/68
Inventor 谭若颖朱苗骏于观贞王杰军
Owner SHANGHAI XIANGQIONG TECHNOLOGY LTD
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