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A composite extracellular matrix component biomaterial

一种生物材料、外基质的技术，应用在复合细胞外基质成分生物材料领域，能够解决机械强度不足、难以制成产品、修复区不稳定等问题，达到促进黏附和迁移、减少凋亡、低免疫原性的效果

Active Publication Date: 2019-03-29
EXCELLENCE MEDICAL TECH SUZHOU CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

②The structure of IT source products is dense, containing a large amount of elastic fibers that degrade slowly and cannot be regenerated after the human body is 25 years old, resulting in long-term instability of the repair area and easy loss of elasticity
However, in the actual production process, the peeling of UBM requires a lot of labor, and the mechanical strength is insufficient, and the surface is smooth and it is difficult to make products with a certain thickness.

Method used

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  • A composite extracellular matrix component biomaterial
  • A composite extracellular matrix component biomaterial
  • A composite extracellular matrix component biomaterial

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Porcine acellular bladder mucosal basement membrane (UBM) and acellular small intestinal submucosa (SIS) were prepared by Abraham's method. A layer of UBM is paved (smooth side down), and the single film-like SIS is spliced ​​into independent sheets according to the interlayer misalignment of 50%, and it is tiled on the surface of the UBM, and the interlayer misalignment is 90°, and 4 layers are laid. A layer of UBM (smooth side up) is laid on it. Remove air bubbles, use medical chitosan adhesive to bond between layers, and then press them into one body with a pressure of -250mm Hg after 24 hours. The whole layer of the material is perforated, the hole spacing is 5mm, and the diameter is 1mm.

Embodiment 2

[0033] Porcine acellular bladder mucosal basement membrane (UBM) and acellular small intestinal submucosa (SIS) were prepared by Abraham's method. The two layers of UBM are paved (smooth side down), and the single film-like SIS is spliced ​​into independent sheets according to the 50% misalignment between the layers, and spread on the surface of the UBM, and 6 layers are laid with a 90° misalignment between the layers. Lay two layers of UBM (smooth side up) on it. Remove the air bubbles, use medical collagen adhesive to bond the layers, and then press them into one body with a pressure of -300mm Hg after 36 hours. The whole layer of the material is perforated, the hole spacing is 8mm, and the diameter is 2mm.

Embodiment 3

[0035] According to GB / T528-2009, take 3 samples and make them into a 4cm×1cm dumbbell shape. After hydration, use a material mechanics testing machine to fix the two ends of the sample and stretch it at a speed of 10mm / min. The tensile strength of the material is measured to be 34±3N / cm.

[0036] Take 3 samples and make them 2cm×5cm, fix the two ends on the upper and lower holders of the tensile machine, and peel them off continuously at a stable speed of 10mm / min until the overlapping parts of the samples are delaminated. Measure the load force when delaminated. The peeling strength between SIS-SIS and UBM-SIS was 6±2N / cm, and the peeling strength was 1.5±0.5N / cm.

[0037] Evaluate the cytotoxicity of the material according to the method specified in GB / T 16886.5. NIH3T3 and L929 were used as model cells. The cells were cultured with the cell culture medium as the leaching medium, and the leaching liquid of gradient concentration was used to replace the culture medium, a...

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PUM

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Abstract

A composite extracellular matrix biomaterial, with a middle layer comprising a decellularized small intestinal submucosa (SIS) (1), and a top layer and a bottom layer comprising a decellularized urinary bladder matrix (UBM) (2). The top and bottom layers completely cover the middle layer, thereby forming a sandwich structure. The sandwich structure allows the UBM (2) to isolate an antigenic property of the SIS (1) and to allowing the UBM to directly contact a host tissue, thereby realizing a high tissue compatibility. The SIS (1) can compensate for a shortcoming of low mechanical strength of the UBM (2). The composite extracellular matrix biomaterial can be used to fill, strengthen, repair, or reconstruct a damage or defect of a fascia, meninges, pleura, pelvic floor, dermis, solid organ, and various types of soft tissues.

Description

technical field [0001] The invention belongs to the field of tissue repair materials, in particular to a composite extracellular matrix component biological material. Background technique [0002] Acellular Tissue Matrix (ACTM) is an important progress in the research of soft tissue repair materials in the past two decades. That is, use physical or chemical methods to remove all cells, antigens, lipids, soluble proteins and other substances in the tissue, and retain the insoluble extracellular matrix (ECM) with complete appearance, histological characteristics and ultrastructure as a biological scaffold. ECM is a highly conserved part in the process of biological evolution. There is little difference in ECM in the same tissue of different species. ACTM after removal of cellular components and antigens that cause immune rejection by decellularization technology can be safely xenotransplanted. Different from the mechanism of synthetic materials that trigger chronic inflammat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/40
CPCA61L27/3604A61L27/3629A61L27/50A61L2430/34A61L27/3633A61L27/3683A61L2430/40A61L27/3691A61L27/3891
Inventor 张剑
Owner EXCELLENCE MEDICAL TECH SUZHOU CO LTD
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