Application of pleutotus ostreatus polysaccharide
A technology of Pleurotus ostreatus polysaccharides and inhibitors, which is applied in the field of medicine and can solve problems such as prevention and treatment that have no relevant reports
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Embodiment 1
[0020] Example 1: Study on the inhibitory effect of Pleurotus ostreatus polysaccharide on acetylcholinesterase in vitro
[0021] 1. Experimental method
[0022] A microplate reader was used to establish an in vitro acetylcholinesterase inhibitory activity screening system, and the in vitro enzyme activity assay method was used to measure the acetylcholinesterase inhibitory activity. 50 Active ingredients are evaluated.
[0023] Microplate reader reaction system:
[0024]
[0025]
[0026] Determination of inhibition rate:
[0027] In the reaction system, the final concentration of the drug in the 96-well plate was set at 100 mg / L, that is, the mother solution was diluted 10 times and added to the reaction system with a volume of 10 μL. The experiment was divided into 6 groups, and each group had 3 duplicate holes, which were respectively negative control groups ( enzyme + buffer + substrate), blank control group (substrate + buffer), positive control group (galantamin...
Embodiment 2
[0034] Example 2: Pharmacodynamic study of Pleurotus ostreatus polysaccharides in improving behavioral disorders of Alzheimer's disease
[0035] 1. Rat modeling and grouping
[0036] Wistar rats, male, weighing 180-220 g, were purchased from the Animal Experiment Center of Bethune School of Medicine, Jilin University, license number: SCXK (Ji) 2013-0001. After one week of adaptive feeding, they were randomly divided into 3 groups, namely: blank group, model group and oyster mushroom polysaccharide group. Except for the blank group, the other two groups were intraperitoneally injected with D-galactose 60 mg / kg and gavaged with AlCl 3 200mg / kg for modeling, once a day for 60 days. From the 30th day, the blank group and the model group were given double distilled water at 10 mL / kg, and the Pleurotus polysaccharide group was given the test drug at 2 g crude drug / kg, once a day for 30 days.
[0037] 2. Platform jumping behavior experiment
[0038]After the last administration,...
Embodiment 3
[0079] Example 3: Effects of Pleurotus ostreatus polysaccharides on the expression of related proteins in the brain of AD rats
[0080] Adopt Western blotting method to be respectively blank group, model group, Pleurotus ostreatus group (grouping with reference to embodiment 2) three kinds of protein levels in brain tissue of rats p-tau, p-GSK3, Aβ are expressed, with GAPDH was used as an internal reference protein, and image J image processing software was used for grayscale analysis.
[0081] Compared with the blank group, the Aβ level and tau and GSK3 phosphorylation levels in the model group increased; compared with the model group, Pleurotus ostreatus can reduce the Aβ level and tau and GSK3 phosphorylation levels in the rat brain, that is, by affecting Aβ metabolism and other related pathways , to improve the cognitive impairment of AD rats, the results are shown in image 3 .
[0082] The changes in the levels of p-tau, p-GSK3, and Aβ in the brains of AD rats were mea...
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