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Detecting method for measuring content of flavonoid compounds and terpene lactone compounds in ginkgo leaf extract or preparations of ginkgo leaf extract at same time

A technology of ginkgo biloba and extract, applied in the field of high-performance liquid chromatography detection, can solve the problems of insufficient process simplicity and complicated operation steps, and achieve the effect of low cost, good durability and easy realization

Active Publication Date: 2016-08-24
HEBEI SHINEWAY PHARMA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method uses different detection methods to detect terpene lactones and flavonol glycosides respectively. The disadvantage is that different instruments and different sample processing conditions are required to inject samples separately, resulting in complicated operation steps and insufficient process simplicity.

Method used

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  • Detecting method for measuring content of flavonoid compounds and terpene lactone compounds in ginkgo leaf extract or preparations of ginkgo leaf extract at same time
  • Detecting method for measuring content of flavonoid compounds and terpene lactone compounds in ginkgo leaf extract or preparations of ginkgo leaf extract at same time
  • Detecting method for measuring content of flavonoid compounds and terpene lactone compounds in ginkgo leaf extract or preparations of ginkgo leaf extract at same time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038](1) Chromatographic conditions: the stationary phase is a chromatographic column filled with octadecyl bonded silica gel; the UV detector is connected in series with the ELSD detector; the UV detection wavelength is 360nm; the flow rate is 1mL / min; the mobile phase A is 0.05% tetrahydrofuran -0.1% formic acid-5% methanol-water mixed solvent, the volume ratio is 1:2:10:950; mobile phase B is a mixed solvent of 0.1% tetrahydrofuran-methanol-acetonitrile solution, the volume ratio is 1:1000, wherein Methanol-acetonitrile solution is a solution with a volume ratio of methanol and acetonitrile of 1:1; gradient elution is performed, and the gradient conditions are:

[0039] time (min)

A(％)

B(％)

0～10

90→84

10→16

10～30

84→80

16→20

30～33

80→79

20→21

33～47

79→79

21→21

47～48

79→76

21→24

48～63

76→67

24→33

63～64

67→60

33→40

64～74

60→45

40→55

74～75

45→90

55→10 ...

Embodiment 2

[0054] Embodiment 2: Ginkgo biloba sample content detection method

[0055] (1) Sample preparation: Take 8 batches of Ginkgo biloba sample powder, weigh 1g precisely, put it in a 10ml volumetric flask, add 70% methanol solution to set the scale mark, and extract by ultrasonic for 30min. The extract was centrifuged at a speed of 14000r / min for 10min, and the supernatant was taken, and then centrifuged at a speed of 14000r / min for 10min, and the supernatant was taken. Take 1ml of the supernatant and blow it with nitrogen to redissolve to 250ul to increase the sample concentration by 4 times, and store it in a refrigerator at 4°C as a stock solution of ginkgo biloba leaves for later use.

[0056] (2) Content detection

[0057] According to the chromatographic conditions described in Example 1, the sample was injected, the peak area was measured, and the corresponding standard line was substituted to calculate the contents of the 11 compounds in the ginkgo biloba sample. The tes...

Embodiment 3

[0060] Embodiment 3: Shuxuening injection content detection method

[0061] (1) Sample preparation: Shuxuening injection was heated at 14000r.min -1 Centrifuge at a high speed for 10min, take the supernatant, and re-centrifuge the supernatant at 14000r.min -1 centrifuge for 10 min at a high speed, take the supernatant, and store it in a refrigerator at 4°C as the stock solution of the Shuxuening injection sample for later use.

[0062] (2) Content detection

[0063] According to the chromatographic conditions described in Example 1, the sample was injected, the peak area was measured, and the corresponding standard line was substituted to calculate the contents of the 11 compounds in the ginkgo biloba sample. The test results of the samples are shown in Table 4.

[0064] Table 4 Shuxuening injection content assay results

[0065]

[0066]

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Abstract

The invention relates to the field of medicine detection, in particular to a high-performance liquid chromatography detecting method for measuring the content of flavonoid compounds and terpene lactone compounds in ginkgo leaves (or ginkgo leaf extract) or preparations of the ginkgo leaves at the same time. The HPLC-DAD-ELSD method is set up, the method can measure the eight flavonoid compounds and three terpene lactone compounds in the ginkgo leaves (or the leaf extract) or preparations of the ginkgo leaves at the same time, the measurement result is analyzed, and a new analysis method and a basis are provided for quality control over the ginkgo leaf extract and the preparations of the ginkgo leaf extract.

Description

technical field [0001] The invention relates to the field of drug detection, more specifically, the invention relates to a high-performance liquid chromatography detection method for simultaneously determining the content of flavonoids and terpene lactones in ginkgo leaves (or its extracts) and preparations thereof. Background technique [0002] Ginkgo is one of the oldest plants on earth. It is a plant of the Ginkgo family Ginkgo family. This tree species has strong vitality. It is not uncommon for a tree to be thousands of years old. It can be called the "old birthday star" in the plant world. It is recorded in "Compendium of Materia Medica" that Ginkgo biloba can invigorate the heart and nourish Qi, nourish the kidney and moisten the lungs, strengthen the brain and refresh the mind, and prolong life. Modern pharmacological studies have shown that Ginkgo biloba has the functions of scavenging oxygen free radicals, lowering blood fat, enhancing central nervous system functi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 常艳旭高秀梅田吉李晋姜海张岩岩李志刚关秀伟
Owner HEBEI SHINEWAY PHARMA
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