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A kind of cultivation method for improving the stability of Cordyceps militaris strain

A cultivation method and stability technology, applied in the cultivation field of improving the stability of Cordyceps militaris strains, can solve problems such as easy degradation and instability of strains, and achieve the effects of fast growth, shortened production cycle, and improved utilization rate

Active Publication Date: 2019-07-23
ALPHA FUJIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of culture method that improves the stability of Cordyceps militaris bacterial classification, overcomes the technical difficulty that bacterial classification is unstable in the existing Cordyceps militaris cultivation, very easy to degenerate, provides abundant and the nutritional labeling of appropriate ratio to improve Cordyceps militaris The culture method of strain stability can not only ensure the stability of the output and quality of Cordyceps militaris, but also shorten the growth cycle and improve the production stability of Cordyceps militaris

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Select the mother species with robust mycelium growth and inoculate it into the prepared test tube slant medium;

[0019] (2) After inoculation, place in an incubator at 22°C for dark culture for 2 days, then at 20°C for 8 hours per day for 3 days, and finally at 18°C ​​for 16 hours per day for 3 days, that is Cultivated species;

[0020] (3) Select cultivars with robust mycelium growth and inoculate them into liquid medium to prepare liquid strains;

[0021] (4) After the inoculation, culture at 20°C for 1 day, then shake it on a shaker, culture at 150r / min and 22°C for 3 days, and finally culture at 20°C and 110r / min for two days. days, the liquid bacteria were obtained;

[0022] (5) Inoculate the liquid strains into the rice culture medium, put them into the cultivation room for routine cultivation, pick them after 35 days of cultivation, and select the young and tender fruiting bodies one week before picking for strain separation. Cultivate in the culture med...

Embodiment 2

[0028] A kind of cultivation method that improves the stability of Cordyceps militaris bacterial classification, described method comprises as follows:

[0029] (1) Select the mother species with robust mycelium growth and inoculate it into the prepared test tube slant medium;

[0030] (2) After inoculation, culture in the dark at 22°C for 1 day in an incubator, then culture at 18°C ​​for 7 hours per day for 2 days, and finally culture at 16°C for 15 hours per day for 2 days, that is Cultivated species;

[0031] (3) Select cultivars with robust mycelium growth and inoculate them into liquid medium to prepare liquid strains;

[0032] (4) After the inoculation, culture at 18°C ​​for 1 day, then vibrate on a shaker, culture at 120r / min and 20°C for 2 days, and finally culture at 18°C ​​and 100r / min for two days. days, the liquid bacteria were obtained;

[0033] (5) Inoculate the liquid strains into the rice culture medium, put them into the cultivation room for routine cultiva...

Embodiment 3

[0039] A kind of cultivation method that improves the stability of Cordyceps militaris bacterial classification, described method comprises as follows:

[0040] (1) Select the mother species with robust mycelium growth and inoculate it into the prepared test tube slant medium;

[0041] (2) After inoculation, culture in the dark at 24°C for 3 days in an incubator, then culture at 22°C for 9 hours per day for 4 days, and finally at 20°C for 18 hours per day for 4 days, that is Cultivated species;

[0042] (3) Select cultivars with robust mycelium growth and inoculate them into liquid medium to prepare liquid strains;

[0043] (4) After the inoculation, culture at 22°C for 2 days, then vibrate on a shaker, culture at 180r / min and 25°C for 4 days, and finally culture at 22°C and 120r / min for two days. days, the liquid bacteria were obtained;

[0044] (5) Inoculate the liquid strains into the rice culture medium, put them into the cultivation room for routine cultivation, and pi...

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Abstract

The invention provides a culture method for improving the stability of a cordyceps militaris strain. The culture method comprises the following steps of: preparing solid and liquid strain culture mediums by utilizing wheat to replace the conventional potato, respectively adding fish meal and dried and peeled shrimp meal into all adopted culture mediums to provide a carbon source, a nitrogen source, vitamins and various mineral elements required in the growth process of cordyceps militaris so as to improve the stability of the strain; propagating and inoculating a mother strain to a prepared inclined culture medium for culture; then selecting a cultivation strain with strong growth of hyphae, inoculating the cultivation strain into the liquid culture medium to culture and prepare a liquid strain; and finally inoculating the liquid strain into a rice culture medium and putting the rice culture medium into a cultivation room for conventional culture. By adoption of the technical scheme, the culture method of cordyceps militaris provided by the invention has the advantages that the stability of the strain can be improved, the continuation of good characters is maintained, and the service life of the strain is about 5 times of that of the common culture method.

Description

technical field [0001] The invention relates to the field of production of Cordyceps militaris, in particular to a cultivation method for improving the stability of the strain of Cordyceps militaris. Background technique [0002] Cordyceps militaris belongs to the phylum Ascomycota, order Hypocreta, Cordyceps family, scientific name Cordyceps militaris , also known as Cordyceps militaris, Cordyceps militaris, etc., belongs to the same category as Cordyceps sinensis, is distributed worldwide, and has a small number of natural resources. Cordyceps militaris contains a variety of biologically active substances that are the same as those of Cordyceps sinensis, including cordycepin, adenosine and other nucleoside substances, as well as polysaccharides from Cordyceps, SOD (superoxide dismutase) and amino acids, among which the most important active ingredients are Cordycepin, studies have shown that cordycepin has various pharmacological activities such as regulating immunity, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00A01G18/20C05G3/00
CPCA01G18/00C05B17/00C05D5/00C05F11/00
Inventor 高益槐王忠李菲
Owner ALPHA FUJIAN BIOTECH
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