Microbial agent for garbage composting as well as preparation method and application thereof
A microbial agent and garbage heap technology, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unstable natural composting and fermentation process, easy to be affected by the external environment, and decline in waste incineration efficiency. Achieve the effect of promoting the increase of the low calorific value of waste, improving microbial activity and low cost
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Embodiment 1
[0041] The microbial agent of the present invention includes Bacillus amyloloquefaciens, Candida utilis, Lactobacillus acidophilus and Rhodopseudomonas palustris. Described Bacillus amyloliquefaciens, Candida utilis, Lactobacillus acidophilus and Rhodopseudomonas palustris are selected from commercially available bacterial preparations, wherein the Bacillus amyloliquefaciens can also be preserved in the China Microorganism Culture Collection Bacillus amyloliquefaciens HFJ-7 under deposit number CGMCC No. 10011 of the Center for General Microorganisms of the Regulatory Commission.
[0042] The depository unit of the Bacillus amyloliquefaciens HFJ-7: General Microbiology Center (CGMCC) of China Microbiological Culture Collection Management Committee; Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; date of deposit : November 19, 2014; the deposit number of Bacillus amyloliquefaciens is CGMCC No. 10011....
Embodiment 2
[0052] The preparation method of the microbial inoculum that is used for garbage heap fermentation described in the present embodiment specifically comprises the following steps:
[0053] 1. Activate Bacillus amyloliquefaciens, Candida utilis, Lactobacillus acidophilus and Rhodopseudomonas palustris respectively in the corresponding medium, and then carry out seed culture in the respective sterilized medium.
[0054] The culture medium of Bacillus amyloliquefaciens is composed of: 10.0 g of peptone, 3.0 g of beef extract, 20.0 g of glucose, 5.0 g of NaCl, 1000 ml of water, and pH 6.8. The inoculum of Bacillus amyloliquefaciens was 8%, and cultured at 30°C for 24 hours. The bacterial content of Bacillus amyloliquefaciens in the Bacillus amyloliquefaciens bacterial liquid obtained after culturing is 40 billion / ml.
[0055] The Candida utilis culture medium is: 10.0 g of yeast extract, 20.0 g of peptone, 20.0 g of glucose, and 1000 ml of distilled water. The inoculum of Candida...
Embodiment 3
[0061] Collect domestic garbage from a garbage collection station in a residential area in Qingdao City, manually break the bags and mix them evenly for use. The physical composition (mass fraction of wet basis) of domestic waste is 74.1% food, 11.5% paper, 4.3% plastic, and 10.1% other. Its initial water content (wet basis) is 66.2%. Treat in a stainless steel container with a height of 120cm and an inner diameter of 40cm, which is covered with a 10cm-thick hollow cotton pad for heat preservation, and a 10cm-thick stone is placed at the bottom as a filter layer to collect leachate, and 50kg (wet basis) of the above-mentioned household garbage were put into 6 experiments In the device, wherein 3 experimental devices were added the microbial inoculum prepared in Example 1 according to the ratio of waste mass to 0.1%, and the other 3 experimental devices were sprayed with water with a mass ratio of 0.1% as a blank control, each In the device, a heavy object of 10kg is loaded on...
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