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Preparation method of probiotic and aloe mask

A technology of probiotics and compound probiotics, applied in the field of preparation of probiotics aloe facial mask, can solve the problems of single effect, hidden safety hazards, high price, etc.

Inactive Publication Date: 2016-08-17
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Nowadays, facial mask has become a must-choice among many skin care products, and occupies a large proportion in the market, but the increase in types, high price and single effect cannot meet the market demand, and some facial masks use various chemical substances to varying degrees. Additives, there are potential safety hazards

Method used

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  • Preparation method of probiotic and aloe mask
  • Preparation method of probiotic and aloe mask

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the fermentation condition of aloe probiotic fermented liquid is determined:

[0029] 1. Preparation of aloe vera juice: select fresh, plump, and fleshy Aloe vera leaves, and remove leaves with diseased spots and soft rot. The average weight of the leaves is about 300-400 g. Wipe the leaf surface with alcohol to remove the epidermis, use a juicer to squeeze the mesophyll into aloe vera juice, subpackage, centrifuge at 6000 rpm / min for 10 min, and take the supernatant. The original aloe juice was sterilized by pasteurization, bathed in water at 55°C for 30 minutes, cooled and stored in a refrigerator at 4°C for future use.

[0030] 2. Fermentation: 6 kinds of probiotics after secondary activation were selected and mixed according to the ratio of 1:1 to prepare a composite probiotic suspension, which was added to the original juice of aloe vera according to the inoculation amount of 5%, supplemented with glucose, and carried out co-fermentation.

[0031] 3....

Embodiment 2

[0032] Embodiment 2: Determination and evaluation of antioxidant index

[0033] 1. Reagents: 0.2 mmol / L DPPH ethanol; 150 mmol / L pH 8.0 Tris-Hcl; 1.2 mmol / L pyrogallol; 0.4% FeSO4: FeSO47H2O; 1% ascorbic acid; 0.2 mol / L sodium hydroxide; 10 % trichloroacetic acid; 0.1% o-phenanthroline; pH 6.6 phosphate buffer; 0.1% ferric chloride.

[0034] 2. After determining the optimum conditions for the aloe fermented liquid, carry out fermentation under the optimum conditions, and store it in a refrigerator at 4°C after fermentation.

[0035] 3. Determination of Antioxidant Index

[0036] 1. Determination of DPPH free radical scavenging ability

[0037] Take 2ml of aloe fermented liquid, add 2ml of 0.2mol / LDPPH ethanol solution, react at room temperature in the dark for 30min, then extract with an equal volume of chloroform, take the supernatant to measure OD at 517nm, the control is deionized water plus DPPH solution.

[0038] 2. Determination of hydroxyl radical scavenging ability ...

Embodiment 3

[0047] Embodiment 3: antibacterial test

[0048] Experimental materials: probiotics, typhoid fever, Salmonella enteritidis, Shiga flexneri, E. coli O157, ATCC, Shiga 301, Staphylococcus aureus cowan1, Aloe vera, LB medium, Oxford cup.

[0049] Experimental steps:

[0050] A Activate probiotics and pathogenic bacteria and culture them overnight.

[0051] B Adjust the concentration of pathogenic bacteria to 10 8 About CFU / mL, take 100 μL of the bacterial solution and spread it on the LB plate.

[0052] C After the bacteria liquid is dried, carefully put a sterile Oxford cup on the plate; add the aloe vera probiotic fermentation liquid to the Oxford cup.

[0053] D Incubate at 37°C for 6-8 h, observe every 2-3 h.

[0054] E Measure the diameter of the inhibition zone in the plate and take a picture.

[0055] The results of Example 1 showed that after fermentation at 37°C, pH=7 for 48 h, and centrifugation at 10,000 rpm / min for 10, the fermentation broth was clear and transl...

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Abstract

The invention provides a preparation method of a probiotic and aloe mask, which is characterized by preparing a fermentation stock solution from aloe vera raw juice, glucose and water according to certain proportions and adding probiotics for performing co-fermentation. By setting different temperature gradients and pH gradients, after viable count, optimal fermentation conditions are determined; an aloe and probiotic fermentation solution is adopted to carry out an antibacterial test so as to determine an antibacterial effect, such as propionibacterium acnes; and meanwhile, by determining an antioxidant index, the antioxidant ability of the aloe and probiotic fermentation solution is determined, such as determination of DPPH radical scavenging ability, determination of iron ion chelating ability and determination of total reducing force. According to the product, the aloe and probiotic fermentation solution is mixed with cosmetic auxiliary materials in proportion so as to be processed into a pasty mask, and after sensory index testing, skin irritation testing and trial effect testing, the safety and functionality of the product are determined, so that the aims of skin protection and health care are achieved.

Description

technical field [0001] The invention relates to a preparation method and effect evaluation of a probiotic aloe facial mask, belonging to the technical field of cosmetic preparation. Background technique [0002] Aloe vera is a natural skin care product, rich in natural vitamins E, C, A and B vitamins, mineral elements, amino acids, aloe polysaccharides, phenolic compounds, etc. plays an important role in. Among them, aloe polysaccharide can regulate the body's cellular immunity and humoral immunity, activate Langerhans cells in the basal layer of the skin, enhance its local immune function and repair function in the skin, promote its removal of skin pigment, and reduce antioxidant damage (including ultraviolet rays) ), anti-aging, increasing skin elasticity and other effects, especially for the occurrence of acne has a good therapeutic effect. [0003] Probiotics refer to bacteria that are beneficial to human and animal organisms. After more than a century of experiments,...

Claims

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Application Information

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IPC IPC(8): A61K8/97A61K8/06A61Q19/00A61Q17/00
CPCA61K8/97A61K8/06A61K2800/85A61Q17/005A61Q19/00
Inventor 陈廷涛辛洪波王鑫周海婷
Owner NANCHANG UNIV
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