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Method for extracting total flavonoids of chrysanthemum

An extraction method and technology of total flavonoids are applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, antidote, etc., and can solve the problems of low extraction rate of active ingredients, low antioxidant activity, and unsuitability for popularization and application. Improve dissolution rate and dissolution times, increase solvent penetration, and speed up the effect

Inactive Publication Date: 2016-02-17
NANYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have the defects of long time-consuming, low extraction rate of active ingredients, complicated operation, and low purity of total flavonoids in the target extract, and low antioxidant activity, so they are not suitable for popularization and application.

Method used

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  • Method for extracting total flavonoids of chrysanthemum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting total flavonoids from chrysanthemums, comprising the following steps:

[0023] (1) using Chrysanthemum chrysanthemum as raw material, drying and crushing to 50 meshes to obtain chrysanthemum chrysanthemum powder;

[0024] (2) Take 40g of white chrysanthemum powder in step (1), add 0.2% cellulase and 0.1% pectinase to the weight of the white chrysanthemum powder, and add 500mL of ethanol solution with a volume concentration of 50%, and adjust the pH value to 4.0~ 5.5, soak for 20 minutes, ultrasonically extract twice, each time for 15 minutes, filter, concentrate until there is no alcohol smell, and obtain the extract and medicinal residue;

[0025] (3) Add 400 mL of distilled water to the dregs of step (2), heat and reflux twice for 4 hours each time, combine the extracts, filter, and concentrate to 200 mL to obtain the extract and dregs;

[0026] (4) Combine the extracts from steps (2) and (3), then add 300mL of petroleum ether, ultrasonically ...

Embodiment 2

[0030] A method for extracting total flavonoids from chrysanthemums, comprising the following steps:

[0031] (1) using Chrysanthemum chrysanthemum as raw material, drying and crushing to 40 meshes to obtain chrysanthemum chrysanthemum powder;

[0032] (2) Take 45g of white chrysanthemum powder from step (1), add 0.3% cellulase and 0.15% pectinase to the weight of the white chrysanthemum powder, and 600mL ethanol solution with a volume concentration of 60%, and adjust the pH value to 4.0~ 5.5, soak for 25 minutes, then ultrasonically extract twice, 30 minutes each time, filter, concentrate until there is no alcohol smell, and obtain the extract and medicinal residue;

[0033] (3) Add 420 mL of distilled water to the medicinal residues in step (2), heat and reflux twice for 3 hours each time, filter, concentrate to 280 mL, and obtain the extract and medicinal residues;

[0034] (4) Combine the extracts from steps (2) and (3), then add 350mL of petroleum ether, perform ultrason...

Embodiment 3

[0038] A method for extracting total flavonoids from chrysanthemums, comprising the following steps:

[0039] (1) using Chrysanthemum chrysanthemum as raw material, drying and crushing to 40 meshes to obtain chrysanthemum chrysanthemum powder;

[0040] (2) Take 47g of white chrysanthemum powder from step (1), add 0.3% cellulase and 0.1% pectinase to the weight of the white chrysanthemum powder, and add 550 mL of ethanol solution with a volume concentration of 65%, and adjust the pH value to 4.0~ 5.5, soak for 30 minutes, then ultrasonically extract twice, 25 minutes each time, filter, concentrate until there is no alcohol smell, and obtain the extract and medicinal residue;

[0041] (3) Add 450 mL of distilled water to the dregs of step (2), heat and reflux 3 times for 3 hours each time, filter, concentrate to 300 mL, and obtain the extract and dregs;

[0042] (4) Combine the extracts from steps (2) and (3), then add 400mL of petroleum ether, ultrasonically extract 3 times, 5...

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Abstract

The invention relates to a method for extracting effective components of chrysanthemum, in particular to a method for extracting total flavonoids of chrysanthemum. Chrysanthemum morifolium ramat as the raw material is dried and smashed, extraction is primarily conducted through enzymes and ultrasonic waves and secondarily conducted through distilled water, two extraction solutions are concentrated, ultrasonic extraction is conducted in petroleum ether, column chromatography isolation is conducted, eluant is collected, concentrating and drying are conducted, and extract of total flavonoids of chrysanthemum is obtained. The method is recyclable in solvent, easy to operate and low in cost; the obtaining rate of extract of total flavonoids of chrysanthemum is larger than or equal to 43%, the purity of total flavonoids is larger than or equal to 82%, the obtaining rate of extract of total flavonoids is high, the purity of total flavonoids is high, the removal capacity of extract of total flavonoids for DPPH is high, and the antioxidant activity of total flavonoids is high.

Description

technical field [0001] The invention relates to a method for extracting active ingredients from chrysanthemums, in particular to a method for extracting total flavonoids from chrysanthemums. Background technique [0002] Chrysanthemum is the flower head of perennial herb of Compositae. It is mostly used for tea, and can also be used as medicine or food additive. Chrysanthemum mainly contains flavonoids, chlorogenic acid, volatile oil and other compounds. The most abundant and most important biological active ingredient is flavonoids Class of compounds, which is a general term for derivatives based on 2-phenylchromone. The flavonoids isolated from chrysanthemum include luteolin, acetin, quercetin, hesperetin, myricetin and so on. Toxicity tests show that chrysanthemum and its extracts are safe and non-toxic, and flavonoids have a wide range of biological activities and pharmacological effects, such as anti-oxidation, anti-aging, anti-cancer, lowering blood fat, lowering chol...

Claims

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Application Information

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IPC IPC(8): A61K36/287A61P39/06
Inventor 李雪晖田龙鲁云风韩雪梅张英君惠丰立夏敏
Owner NANYANG NORMAL UNIV
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