Method for continuously preparing silkworm chrysalis protein antioxidant peptides by using enzyme membrane reactor

Abstract

The invention relates to a method for continuously preparing silkworm chrysalis protein antioxidant peptides by using an enzyme membrane reactor. The method comprises the following steps of: firstly, preparing silkworm chrysalis protein into a suspension by using distilled water; secondly, homogenizing the suspension by using a homogenizer, and then adding the suspension together with incision enzyme into the enzyme membrane reactor for continuously preparing antioxidant peptides; thirdly, penetrating the enzymolysis product solution through an ultrafiltration membrane to ensure that the silkworm chrysalis protein which is not fully reacted and the incision enzyme are captured and then reflow into a reaction tank for continuous reaction; and finally, collecting the enzymolysis product penetrating fluid penetrating through the ultrafiltration membrane, and concentrating and then carrying out spray drying to obtain a powdered silkworm chrysalis protein antioxidant peptide product. The silkworm chrysalis protein antioxidant peptide prepared by the method is good in activity, the IC50 for clearing DPPH (1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl) free radical is 0.9-1.6mg / mL, the IC50 of the capacity of clearing hydroxy radical is 0.2-0.6mg / mL, and the IC50 of the capacity of chelating Fe<2+> is 1.4-2.7mg / mL.

Description

technical field [0001] The invention relates to the field of food biochemistry and can be used as a functional additive in food, cosmetics and medicines, in particular to a method for continuously preparing silkworm chrysalis protein antioxidant peptides using an enzyme membrane reactor. Background technique [0002] Free radicals are related to many chronic human diseases such as cancer, coronary heart disease, and Alzheimer's disease. Therefore, eliminating free radicals in the body can improve the body's resistance and preventive capabilities against diseases, and has attracted widespread attention from all walks of life. Synthetic antioxidants such as butyl hydroxyanisole (BHA) and 2,6-di-tert-butyl-p-methylphenol (BHT) are often used as food additives for preservation, but synthetic antioxidants have harmful effects on human health. Therefore, the development of highly efficient and safe antioxidants has become a research hotspot. As a kind of biologically active pepti...

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Problems solved by technology

[0004] Existing studies all use intermittent enzymatic hydrolysis to prepare silkworm chrysalis protein antioxidant peptides. This method has disadvantages such as low utilization rate of protease, long production cycle, unstable product quality and high labor intensity, and is not suitable for industrial production.

Enzyme-membrane reactors can solve these problems very well. There are not many reports about the use of enzyme-membrane reactors to prepare antioxidant peptides. (Application No.: 201010188410.5)" and "A Method for Preparing Oat Antioxidant Peptides Using an Enzyme Membrane Reactor (Application No.: 201110112515.7) have been publicly reported. There is no report on the continuous preparation of silkworm chrysalis protein antioxidant peptides using an enzyme membrane reactor.

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