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Preparation method of human measles immune sensor based on graphdiyne and silver nanoparticle cage

An immunosensor and silver nanocage technology, which is applied in the preparation of human measles immunosensor based on graphyne and silver nanocage, can solve the impact of quantitative determination, interfere with the determination of bioavailability, affect the clearance and pharmacology of protein and polypeptide drugs Effect and other issues, to achieve ultra-sensitive and stable detection, promote electron transfer, increase the effect of electron transfer energy

Active Publication Date: 2016-08-10
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ELISA has many disadvantages, such as interference from metabolites, which are cross-reactive in the immunoassay of the prototype drug, which can significantly interfere with the determination of bioavailability; or interference from antibody formation, which can affect protein The clearance and pharmacological effects of peptide drugs, and their impact on quantitative determination

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A preparation method of human measles immunosensor based on graphdiyne and silver nanocage

[0023] (1) A glassy carbon electrode with a diameter of 3 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water; adding 5 µL, 0.7 mg / mL graphyne@thionineGy@TH composite material dropwise to the surface of the electrode, rinsed with ultrapure water, and dried at room temperature to form a film;

[0024] (2) Drop 5 µL of silver nanocages on the surface of the electrode, rinse with ultrapure water, and dry at room temperature;

[0025] (3) Add 5 µL of human measles IgM antibody and 5 µL of bovine serum albumin BSA solution with a mass fraction of 0.5% onto the surface of the electrode, rinse with ultrapure water, and dry in a 4°C refrigerator;

[0026] (4) Add 5 µL, 0.003-2.8 ng / mL of a series of human measles IgM antigen standard solutions of different concentrations to the surface of the electrode, rinse with ultrapure water, and dry in a ref...

Embodiment 2

[0028] A preparation method of human measles immunosensor based on graphdiyne and silver nanocage

[0029] (1) A glassy carbon electrode with a diameter of 3 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water; adding 6.5 µL, 2 mg / mL graphyne@thionineGy@TH composite material dropwise to the surface of the electrode, rinsed with ultrapure water, and dried at room temperature to form a film;

[0030] (2) Drop 6.5 µL of silver nanocages on the surface of the electrode, rinse with ultrapure water, and dry at room temperature;

[0031] (3) Add 6.5 µL of human measles IgM antibody and 6.5 µL of bovine serum albumin BSA solution with a mass fraction of 3% to the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0032] (4) Add 6.5 µL, 0.003 ~ 2.8 ng / mL of a series of human measles IgM antigen standard solutions of different concentrations to the surface of the electrode, rinse with ultrapure water, and dry...

Embodiment 3

[0034] A preparation method of human measles immunosensor based on graphdiyne and silver nanocage

[0035] (1) A glassy carbon electrode with a diameter of 3 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water; adding 10 µL, 5 mg / mL graphyne@thionineGy@TH composite material dropwise to the surface of the electrode, rinsed with ultrapure water, and dried at room temperature to form a film;

[0036] (2) Drop 10 µL of silver nanocages on the surface of the electrode, rinse with ultrapure water, and dry at room temperature;

[0037] (3) Add 10 µL of human measles IgM antibody and 10 µL of bovine serum albumin BSA solution with a mass fraction of 5% to the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0038] (4) Add 10 µL, 0.003-2.8 ng / mL of a series of human measles IgM antigen standard solutions of different concentrations to the surface of the electrode, rinse with ultrapure water, and dry in a r...

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Abstract

The invention relates to a preparation method of a human measles immune sensor based on graphdiyne and a silver nanoparticle cage, and belongs to the technical field of novel biological sensing and detection. Graphdiyne is of a carbon-carbon triple-bond linear structure with an sp hybridized state and has the advantages of zero cis-trans-isomer, high conjugation and the like; due to excellent electrical, optical and photoelectric properties, graphdiyne is a key material of a novel electronic and photoelectric device of the next generation. Meanwhile, by the use of good pi-pi conjugation effect, a high-flux load of signal molecules is realized, and a signal error caused by instable combination is reduced. Therefore, the human measles immune sensor based on graphdiyne and the silver nanoparticle cage can sensitively detect measles viruses.

Description

technical field [0001] The invention relates to a preparation method of a human measles immunosensor based on graphyne and silver nanocages. The graphyne@thionineGy@TH composite material is used as the detection base material to enhance the conductivity and make the signal molecule thionine successful. An electrochemical immunosensor for detecting human measles is prepared by being fixed on a sensing surface through the cross-linking action of silver nanocages, which belongs to the technical field of new functional materials and biosensing detection. Background technique [0002] Measles virus is the pathogen of measles. It is a common acute infectious disease in children. It is highly contagious and characterized by skin papules, fever and respiratory symptoms. Measles remains a significant cause of child mortality, especially in developing countries. Therefore, the pre-morbid sensitive detection of measles is particularly important. [0003] At present, there are not man...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N33/569
CPCG01N27/308G01N27/3278G01N33/56983G01N2333/12
Inventor 任祥魏琴吴丹马洪敏庞雪辉李燕张勇
Owner UNIV OF JINAN
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