Porous calcium phosphate/gelatin composite scaffold for bone tissue engineering and preparation method of porous calcium phosphate/gelatin composite scaffold

Abstract

The invention relates to a porous calcium phosphate / gelatin composite scaffold for bone tissue engineering and a preparation method of the porous calcium phosphate / gelatin composite scaffold, and belongs to the field of application of a biomedical material technology. The composite scaffold is prepared by the following steps: steeping organic form obtained by laser processing and molding as a carrier into slurry containing calcium phosphate powder, and then carrying out the processes of heating, drying, thermal treatment and the like to prepare the porous calcium phosphate scaffold combining irregular through holes and regular straight through holes; and compounding the prepared porous calcium phosphate scaffold and gelatin to obtain the porous calcium phosphate / gelatin composite scaffold for bone tissue engineering, wherein the composite scaffold has a hole structure combining the irregular through holes and the regular straight through holes; the porosity is 70-95%; the apertures of the irregular through holes are 50-600 microns; and the apertures of the regular straight through holes are 0.4-1.8mm. The method is simple in process, convenient to operate and stable in yield; and the prepared scaffold is qualified in cytotoxicity and good in osteogenesis effect and has a good application prospect.

Description

technical field [0001] The invention relates to a porous calcium phosphate / gelatin composite scaffold for bone tissue engineering and a preparation method thereof, belonging to the technical application field of biomedical materials. Background technique [0002] With the deteriorating global ecological environment, there are more and more cases of bone defects caused by infection, tumor, trauma, etc., people's demand for synthetic biomaterials is increasing, and more and more researchers are investing a lot of energy and Material research and development of bone repair or bone replacement materials, therefore, calcium phosphorus ceramics with good bioactivity, biocompatibility, osteoconductivity, etc., have been widely studied as bone repair or replacement materials, but how to synthesize them with natural bone strength Calcium-phosphorus ceramic materials, which are similar, have good cell interface compatibility, and have a consistent degradation rate with the bone format...

AI Technical Summary

Problems solved by technology

[0007] At present, the methods used to prepare porous biological scaffolds mainly include adding pore-forming agent method, organic foam impregnation method, foaming reaction method, sol-gel method, etc. Among them, it is difficult to obtain high porosity by adding pore-forming agent method, and it is easy to cause The pore size distribution of the scaffold material is not uniform; the foaming method is difficult to control the distribution of pores, and the mechanical strength of the porous body is often not high; while the foam impregnation method has a simple process, and the prepared porous material has a high porosity (70-90%). It is controllable and has a three-dimensional open-pore network structure, which can adapt to a certain range of stress changes. At the same time, the porous tissue can allow blood to circulate and ensure the normal growth of bone tissue, which can better meet the requirements of bone tissue engineering scaffold materials for porous structures. However, the bioceramic scaffolds prepared by the foam impregnation method used today are all three-dimensional through-hole structures. How to optimize the pore structure of the three-dimensional through-porous ceramics is a hot and difficult point of current research.

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