High static pressure and enzyme hydrolysis combined method for preparing hypoallergenic ginkgo protein powder
A technology with high static pressure combined with enzymes, applied in food science, fermentation, etc., to achieve the effects of high safety, good solubility, and simple process operation
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Embodiment 1
[0033] High static pressure treatment of ginkgo protein
[0034] Take a certain amount of ginkgo protein, prepare a ginkgo protein solution with a concentration of 40-60 mg / mL with distilled water, seal it in a double-layer polyethylene plastic bag, vacuum seal it, put it in a high-pressure device, and store it at 25°C. Under high static pressure treatment, the treatment pressure range is 100-700MPa, and the treatment time is 20-30min.
[0035] The present invention studies the influence of different treatment pressures on the sensitization of ginkgo protein. 50 g of ginkgo protein is taken respectively, and distilled water is used to prepare a ginkgo protein solution with a concentration of 50 mg / mL, which are respectively sealed in double-layer polyethylene plastic bags, and pumped out. After being vacuum-sealed, place it in a high-pressure equipment, and use 100, 200, 300, 400, 500, 600, and 700 MPa to perform high-static pressure treatment at 25°C for 20 minutes, and then us...
Embodiment 2
[0038] Protease Hydrolysis of Ginkgo Protein
[0039] Take a certain amount of high-pressure-treated ginkgo protein sample, heat-treat it at 90°C for 5 minutes, and then use 1mol / L HCl or 1mol / L NaOH to adjust the pH value of the solution to the optimum hydrolysis pH for protease. According to the quality of ginkgo protein sample, press 1~ Add protease to 5% mass ratio for hydrolysis. The hydrolysis temperature is the optimum hydrolysis temperature of protease. The hydrolysis time is 1-2 hours. After hydrolysis, the enzyme activity is inactivated at 95°C for 10 minutes. Ginkgo protein powder.
[0040] The protease is one of alkaline protease, papain, pepsin or neutral protease.
[0041] The optimum hydrolysis pH of the protease is pH 8.0 for alkaline protease, pH 6.5 for papain, pH 1.5 for pepsin and pH 7.0 for neutral protease.
[0042] The optimum hydrolysis temperature for protease is 45°C for alkaline protease, 55°C for papain, 40°C for pepsin and 45°C for neutral protea...
Embodiment 3
[0046] Preparation of Hypoallergenic Ginkgo Protein Powder
[0047] (1) Separation and purification of ginkgo protein
[0048] Take 1kg of fresh ginkgo fruit, shell them, freeze-dry them, grind and sieve them, and degrease them with petroleum ether until the degreasing is complete. After the petroleum ether is completely volatilized, add pH7.4 at a ratio of 1:15g / mL to a concentration of 0.2mol / L The Tris-HCl solution was used for protein extraction, magnetically stirred at 4°C for 24h, then refrigerated and centrifuged at 5000r / min for 15min at 4°C, and the supernatant was passed through 40 and 80g / 100mL saturated (NH4) 2 SO 4 Carry out fractional precipitation, refrigerate and centrifuge at 5000r / min for 30min at 4°C, dissolve the precipitate with a small amount of Tris-HCl solution with a concentration of 0.01mol / L at pH 8.0, place it in a dialysis bag with a molecular weight cut-off of 3.5kDa and use the same Tris- HCl solution was dialyzed, and after the dialysate was f...
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