Method for culturing cordyceps militaris with slant solid strains effectively instead of liquid strains

Abstract

The invention discloses a method for culturing cordyceps militaris with slant solid strains effectively instead of liquid strains. The method comprises the following steps: culturing carrot slant cordyceps militaris strains, wherein a culture medium is prepared from the following components in parts by weight: 200 parts of juice prepared from carrots, 20 parts of glucose, 0.5 part of magnesium sulfate, 1 part of monopotassium phosphate and 20 parts of agar; performing subpackaging, sterilization, cooling, inoculation, culturing and selection on the culture medium; aseptically transferring the slant strains into a one-off injection syringe, sucking glucose injection, extruding a solution obtained by mixing the slant stains and the glucose injection from a syringe needle, pouring the extruded solution back into the one-off injection syringe, repeating the operation until the carrot slant cordyceps militaris strains are completely and uniformly mixed with the glucose injection, and inoculating prepared solidified cordyceps militaris liquid strains into a food culture medium or silkworm pupae; culturing the food culture medium or the silkworm pupae in a constant temperature incubator. The method is easy to operate, short in culture period, high in fruiting body yield and free of pollution and waste, and factory production can be implemented.

Description

Technical field: [0001] The invention relates to a cordyceps militaris culturing method that can effectively replace liquid strains with solid strains of cordyceps militaris, and belongs to the field of biotechnology. Background technique: [0002] Cordyceps militaris, also known as Cordyceps militaris, belongs to the genus Cordyceps of the ergotaceae, and is one of the rare Chinese herbal medicines in my country. Modern pharmaceutical research has shown that Cordyceps militaris contains nucleosides, polysaccharides, cordycepin, sterols and other ingredients basically the same as Cordyceps sinensis. It has therapeutic effects on immune system, nervous system and cardiovascular system diseases, and has anti-tumor and Anti-cancer effect, it is an ideal substitute for Cordyceps sinensis and has broad prospects for development. [0003] At present, most production companies use liquid strains to produce Cordyceps militaris in factories, but due to strain degradation, the strains used ...

AI Technical Summary

Problems solved by technology

However, there are many problems in this method. For example, in the late stage of the cultivation process, the liquid bacteria have more extraspore enzymes that affect the measurement results; this method has a lot to do with the scientific quality of the personnel and the precision of the instrument. Many companies will invest most of their funds to reduce profits

And Cordyceps militaris can produce a large amount of conidia in the liquid culture medium, it is also difficult to observe the conidia content in the culture medium

[0004] There are two kinds of Cordyceps militaris with different names in the market, one is called Cordyceps flower, this kind of Cordyceps militaris is made in the grain culture medium, although it is said that the high content of cordycepin has medicinal value, but it is not listed in China Therefore, the price in the Pharmacopoeia is lower; the other is called Cordyceps militaris or Cordyceps militaris. This kind of Cordyceps militaris grows in the body of silkworm chrysalis and tends to sell better in the wild, and it is included in the Chinese Pharmacopoeia, so the price is higher, but it is more difficult to cultivate. Large, high cost makes it impossible to carry out large-scale factory production

[0005] Now in the cultivation process of silkworm chrysalis Cordyceps militaris, the ossification rate of pupae directly affects the yield rate, which in turn affects the economic income of production. Now the cultivation of the mother species in the cultivation process of Cordyceps militaris is mainly made of potato, beef extract, and peptone as the main raw materials. The nutrient composition is single, the growth rate of the strain is slow, and genetic variation is prone to occur at the same time, the ossification rate of the pupa is low, and the yield is unstable.