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Acid-resistant bacterial cellulose high-yielding strain and method for preparing edible bacterial cellulose with same

A technology of bacterial cellulose and bacterial strains, which is applied in the field of bacterial cellulose high-yielding bacteria and edible bacterial cellulose prepared by the bacterial strain, can solve the problems of few research reports on the fermentation process of bacterial cellulose high-yielding bacteria, and achieve low cost and high yield. High efficiency and good quality effect

Active Publication Date: 2016-06-29
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, many modern researchers have done a lot of research on bacterial cellulose strain screening, raw material selection, fermentation process, etc., but there are few research reports on the breeding of bacterial cellulose high-yielding bacteria, fermentation substrate, and fermentation process.

Method used

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  • Acid-resistant bacterial cellulose high-yielding strain and method for preparing edible bacterial cellulose with same
  • Acid-resistant bacterial cellulose high-yielding strain and method for preparing edible bacterial cellulose with same

Examples

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Embodiment 1

[0021] Example 1: Screening, isolation and identification of Gluconacetobacter xylinum JX1a

[0022] Collect Hainan coconut milk, let it ferment naturally (that is, self-ferment under natural conditions) for 15 days, draw 1ml into a 10ml test tube, and then add 9ml sterile water to make 10 -1 Concentration of bacterial suspension, diluted sequentially, respectively from 10 -2 、10 -3 Draw 200 μl of the concentration gradient dilution solution and spread it evenly on the plate. Make three controls for each concentration gradient, and place it in a 30°C incubator to observe the cultivation situation. After 4 days of cultivation, pick the colony that grows well, distributes evenly, and produces a transparent circle. All single colonies were inoculated on solid slant medium, and incubated at 30°C for 24-48 hours. After a thick lawn grows on the slant, pick a ring of bacteria from the slant and inoculate it in 10ml of fermentation broth and incubate at a constant temperature of 30...

Embodiment 2

[0030] Embodiment 2: the preparation method of edible bacterial cellulose

[0031] Inoculate the slant seeds into a 10mL test tube (seed medium glucose 2%, yeast extract 0.5%, K 2 HPO 4 0.2%, MgSO 40.15%, ethanol 2%), cultured in a constant temperature incubator at 30°C for 24 hours, and after the fibrous membrane grew on the surface, it was transferred to 100mL seed medium according to the inoculum size of 10%, and incubated at 30°C The primary seed liquid was obtained by statically culturing in a constant temperature incubator for 3 days.

[0032] Fermented seed culture: the primary seed liquid is inoculated in the fermented seed culture medium by 10% inoculum size (glucose 80g / L, coconut milk 20g / L, glacial acetic acid 13g / L, soybean peptone 0.01g / L, magnesium sulfate heptahydrate 0.2g / L, pH2.5), cultured statically in a 30°C incubator for 3 days.

[0033] Shallow pan static fermentation: the fermented seed liquid is received in the fermentation medium according to the ...

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Abstract

The invention provides a strain for producing bacterial cellulose. The strain is classified and named as JX1a(Komagataeibacter xylineus JX1a), the preservation unit is China Center for Type Culture Collection, the preservation number is CCTCC NO:M 2015195, the preservation date is April 6, 2015, and the preservation address is Wuhan University, Wuhan, China. The strain has high pH adaptive capacity, and the stain can grow well under the condition that the the pH ranges from 2.5 to 3.0; the strain can produce high-quality edible dietary fibers in a dairy culture medium containing coconut milk, milk, soy milk and the like, and the strain can also be used for producing bacterial cellulose masks and medical materials.

Description

technical field [0001] The invention relates to the field of food biotechnology, in particular to a bacterial cellulose high-yielding bacterium and the use of the bacterial strain to prepare edible bacterial cellulose. Background technique [0002] Bacterial cellulose (BC) is a three-dimensional network structure with high crystallinity formed by the metabolic products of various bacteria without lignin, hemicellulose and other extracts. This structure makes BC have some unique characteristics. Such as high purity, high crystallinity, greater mechanical strength, high water retention value, antibacterial, non-toxic, biocompatibility and biodegradability, etc., so that it can be used in many fields It has a wide range of applications, such as biomedicine and tissue engineering, food, electricity, papermaking, functional materials, etc. [0003] Bacterial cellulose is a water-insoluble dietary fiber, which can change the rheological state of food and produce low-calorie, low-...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04A23L33/24C12R1/01
CPCA23V2002/00C12N1/20C12P19/04C12N1/205C12R2001/01A23V2250/5108
Inventor 于秋生冯伟白爱娟李珍妮徐晖
Owner JIANGNAN UNIV
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